{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T11:14:20Z","timestamp":1775646860829,"version":"3.50.1"},"reference-count":13,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:00:00Z","timestamp":1617321600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005073","name":"Agency for Defense Development","doi-asserted-by":"publisher","award":["UE202026GD"],"award-info":[{"award-number":["UE202026GD"]}],"id":[{"id":"10.13039\/501100005073","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Armed Forces Health Surveillance Branch, Global Emerging Infections Surveillance and Response System","award":["P0025-2016-2018-ME"],"award-info":[{"award-number":["P0025-2016-2018-ME"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of Orientia tsutsugamushi 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the O. tsutsugamushi aligned most closely with the Boryong strain.<\/jats:p>","DOI":"10.3390\/pathogens10040424","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T10:34:09Z","timestamp":1617359649000},"page":"424","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of Orientia tsutsugamushi Using Multiplex PCR-Based Next-Generation Sequencing"],"prefix":"10.3390","volume":"10","author":[{"given":"Seungchan","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon C.","family":"Allison","sequence":"additional","affiliation":[{"name":"Force Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kkothanahreum","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Sun","family":"No","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung-Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2132-0674","authenticated-orcid":false,"given":"Kyungmin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jongwoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry A.","family":"Klein","sequence":"additional","affiliation":[{"name":"Force Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7167-2045","authenticated-orcid":false,"given":"Heung-Chul","family":"Kim","sequence":"additional","affiliation":[{"name":"Force Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3178-8569","authenticated-orcid":false,"given":"Won-Keun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Korea"},{"name":"Institute of Medical Science, College of Medicine, Hallym University, Chuncheon 24252, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Won","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1086\/516990","article-title":"A Case of scrub typhus probably acquired in Africa","volume":"25","author":"Ghorbani","year":"1997","journal-title":"Clin. 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