{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:11:50Z","timestamp":1765807910809,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T00:00:00Z","timestamp":1619568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea","award":["NRF- 2019R1C1C1002170"],"award-info":[{"award-number":["NRF- 2019R1C1C1002170"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>In traditional Chinese medicine, Ranunculus japonicus has been used to treat various diseases, including malaria, and the young stem of R. japonicus is consumed as a food in the Republic of Korea. However, experimental evidence of the antimalarial effect of R. japonicus has not been evaluated. Therefore, the antimalarial activity of the extract of the young stem of R. japonicus was evaluated in vitro using both chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains; in vivo activity was evaluated in Plasmodium berghei-infected mice via oral administration followed by a four-day suppressive test focused on biochemical and hematological parameters. Exposure to extracts of R. japonicus resulted in significant inhibition of both chloroquine-sensitive (3D7) and resistant (Dd2) strains of P. falciparum, with IC50 values of 6.29 \u00b1 2.78 and 5.36 \u00b1 4.93 \u03bcg\/mL, respectively. Administration of R. japonicus also resulted in potent antimalarial activity against P. berghei in infected mice with no associated toxicity; treatment also resulted in improved hepatic, renal, and hematologic parameters. These results demonstrate the antimalarial effects of R. japonicus both in vitro and in vivo with no apparent toxicity.<\/jats:p>","DOI":"10.3390\/pathogens10050532","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T22:29:07Z","timestamp":1619648947000},"page":"532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus"],"prefix":"10.3390","volume":"10","author":[{"given":"Hae-Soo","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6127-5356","authenticated-orcid":false,"given":"Sylvatrie-Danne","family":"Dinzouna-Boutamba","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Zoonotic and Vector Borne Disease Research, Center for Infectious Diseases, Korea National Institute of Health, Korea CDC, Chungbuk 28159, Korea"},{"name":"Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zin","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0876-3179","authenticated-orcid":false,"given":"Dongmi","family":"Kwak","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3088-1318","authenticated-orcid":false,"given":"Man-Hee","family":"Rhee","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Il","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeonchul","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youn-Kyoung","family":"Goo","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,28]]},"reference":[{"unstructured":"Xie, Z.W. 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