{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:02:52Z","timestamp":1760234572470,"version":"build-2065373602"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: S. enterica subsp. houtenae has been rarely documented, and very limited genomic information is available. This report describes a rare case of primary extraintestinal salmonellosis in a young roe deer, associated with Salmonella enterica subsp. houtenae.\u00a0Methods: A traditional cultural-based analysis was carried out from the contents of a neck abscess; biochemical identification and PCR assay were performed to isolate and identify the pathogen. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), core genome MLST (cgMLST), and the Salmonella pathogenicity islands (SPIs) survey, resistome and virulome genes were investigated to gain insight into the virulence and antimicrobial resistance of S. houtenae. Results: Biochemical identification and PCR confirmed the presence of Salmonella spp. in the swelling. The WGS analysis identified Salmonella enterica subspecies houtenae serovar 43:z4,z23:- and ST 958. The virulence study predicted a multidrug resistance pattern with resistance shown against aminoglycosides, tetracycline, beta-lactamase, fluoroquinolones, fosfomycin, nitroimidazole, aminocoumarin, and peptide. Fifty-three antibiotic-resistant genes were identified. No plasmids were detected. Conclusion: This study demonstrates the importance of continuous surveillance of pathogenic salmonellae. Biomolecular analyses combined with epidemiological data can provide important information about poorly described Salmonella strains and can help to improve animal welfare.<\/jats:p>","DOI":"10.3390\/pathogens10060654","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T22:02:23Z","timestamp":1621980143000},"page":"654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Salmonella enterica Subsp. houtenae Associated with an Abscess in Young Roe Deer (Capreolus capreolus)"],"prefix":"10.3390","volume":"10","author":[{"given":"Adriana","family":"Trotta","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\u201d, Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2729-9933","authenticated-orcid":false,"given":"Laura","family":"Del Sambro","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e Basilicata, Sezione di Putignano, Contrada San Pietro Piturno, 70017 Putignano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3386-4879","authenticated-orcid":false,"given":"Michela","family":"Galgano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\u201d, Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4487-5784","authenticated-orcid":false,"given":"Stefano","family":"Ciccarelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\u201d, Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Ottone","sequence":"additional","affiliation":[{"name":"Parco Nazionale Pollino, Complesso Monumentale Santa Maria della Consolazione, 85048 Rotonda, PZ, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2858-9809","authenticated-orcid":false,"given":"Domenico","family":"Simone","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e Basilicata, Sezione di Putignano, Contrada San Pietro Piturno, 70017 Putignano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2744-1140","authenticated-orcid":false,"given":"Antonio","family":"Parisi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e Basilicata, Sezione di Putignano, Contrada San Pietro Piturno, 70017 Putignano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-3831","authenticated-orcid":false,"given":"Domenico","family":"Buonavoglia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\u201d, Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marialaura","family":"Corrente","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\u201d, Str. Prov. per Casamassima Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.cimid.2012.11.004","article-title":"Prevalence and antimicrobial susceptibility of Salmonella in European wild boar (Sus scrofa); Latium Region\u2014Italy","volume":"36","author":"Zottola","year":"2013","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"EFSA (European Food Safety Authority), and ECDC (European Centre for Disease Prevention and Control) (2019). 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