{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:03:55Z","timestamp":1791266635571,"version":"4.1.0"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T00:00:00Z","timestamp":1622678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005883","name":"Tanzania Commission for Science and Technology","doi-asserted-by":"publisher","award":["CST\/NFAST-SIDA\/CRG\/2015"],"award-info":[{"award-number":["CST\/NFAST-SIDA\/CRG\/2015"]}],"id":[{"id":"10.13039\/501100005883","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Leptospirosis is among the major neglected zoonoses in developing countries. The prevalence of leptospirosis remains underestimated in many African countries because of limited diagnostic facilities. We studied Leptospira seropositivity prevalence in humans, sheep, goats and rodents in a semi-arid region of central Tanzania and compared findings with reports from humid tropical areas. The aims were to establish the disease burden in different settings; understand circulating Leptospira serovars and potential major reservoirs for establishing appropriate control measures. Methods: Humans, sheep, goats, rodents and shrews (insectivores) were sampled from Bahi district, a semi-arid area in central Tanzania. Samples were tested for leptospiral antibodies using microscopic agglutination test (MAT) consisting of Leptospira serovars mainly reported in Tanzania and reference strains. Findings were compared with previous data to determine the disease epidemiological patterns. Results and conclusion: Semi-arid area showed high Leptospira seropositivity prevalence in humans and domestic animals due to intensive human\u2013animal interactions at scarce water points and by flash flooding which occur in the area. Rodent population in the semi-arid areas was relatively low due to flooding. Leptospira seropositivity in rodents was also slightly lower, and the rodents appeared to be prolific breeders, probably as a means to compensate for the lost population during extreme drought as well as during short spells of floods. Intensive human\u2013animal interaction in the semi-arid areas especially, in water sources in valleys where human and animals often meet, likely increased the risk of leptospirosis transmission to rice farmers in the area. Goats and sheep which are kept around homesteads had higher leptospiral antibodies prevalence (62%), nearly double of the 38% reported in same species in humid tropical regions of Tanzania. Livestock, especially goats and sheep, could be the major source of leptospirosis transmission to humans. Vaccination of livestock with vaccines against local Leptospira strains should be encouraged, and rodent control emphasized, as part of a management strategy against leptospirosis. Public awareness of leptospirosis must also be raised and supported by availability of rapid test kits in clinics for preliminary testing of leptospirosis in people with fevers of unknown origin.<\/jats:p>","DOI":"10.3390\/pathogens10060696","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T21:03:32Z","timestamp":1622754212000},"page":"696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Leptospira Seropositivity in Humans, Livestock and Wild Animals in a Semi-Arid Area of Tanzania"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-5644","authenticated-orcid":false,"given":"Georgies F.","family":"Mgode","sequence":"first","affiliation":[{"name":"Pest Management Centre, Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development, Sokoine University of Agriculture, P.O. Box 3110 Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginethon G.","family":"Mhamphi","sequence":"additional","affiliation":[{"name":"Pest Management Centre, Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development, Sokoine University of Agriculture, P.O. Box 3110 Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apia W.","family":"Massawe","sequence":"additional","affiliation":[{"name":"Pest Management Centre, Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development, Sokoine University of Agriculture, P.O. Box 3110 Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7274-8243","authenticated-orcid":false,"given":"Robert S.","family":"Machang\u2019u","sequence":"additional","affiliation":[{"name":"Pest Management Centre, Africa Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development, Sokoine University of Agriculture, P.O. Box 3110 Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,3]]},"reference":[{"key":"ref_1","unstructured":"Mgode, G.F. (2017). Unpublished work."},{"key":"ref_2","unstructured":"World Health Organization (2003). Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control, World Health Organization."},{"key":"ref_3","first-page":"97","article-title":"Leptospirosis in animals and humans in selected areas of Tanzania","volume":"127","author":"Mgode","year":"1997","journal-title":"Belg. J. Zool."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"275","DOI":"10.4269\/ajtmh.2011.11-0176","article-title":"Leptospirosis among Hospitalized Febrile Patients in Northern Tanzania","volume":"85","author":"Biggs","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Biggs, H.M., Hertz, J.T., Munishi, O.M., Galloway, R.L., Marks, F., Saganda, W., Maro, V.P., and Crump, J.A. (2013). Estimating Leptospirosis Incidence Using Hospital-Based Surveillance and a Population-Based Health Care Utilization Survey in Tanzania. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002589"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Crump, J.A., Morrissey, A.B., Nicholson, W.L., Massung, R.F., Stoddard, R.A., Galloway, R.L., Ooi, E.E., Maro, V.P., Saganda, W., and Kinabo, G.D. (2013). Etiology of Severe Non-malaria Febrile Illness in Northern Tanzania: A Prospective Cohort Study. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002324"},{"key":"ref_7","unstructured":"Allan, K.J. (2016). Leptospirosis in Northern Tanzania: Exploring the Role of Rodents and Ruminant Livestock in a Neglected Public Health Problem. [Ph.D. Thesis, University of Glasgow]."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mgode, G.F., Machang\u2019u, R.S., Mhamphi, G.G., Katakweba, A., Mulungu, L.S., Durnez, L., Leirs, H., Hartskeerl, R.A., and Steven, R. (2015). Belmain. Leptospira Serovars for Diagnosis of Leptospirosis in Humans and Animals in Africa: Common Leptospira Isolates and Reservoir Hosts. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004251"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1099\/ijs.0.63278-0","article-title":"New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania","volume":"56","author":"Mgode","year":"2006","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.4314\/thrb.v16i1.4","article-title":"Seroprevalence of Leptospira infection in bats roosting in human settlements in Morogoro municipality in Tanzania","volume":"16","author":"Mgode","year":"2014","journal-title":"Tanzan. J. Health Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112","DOI":"10.4314\/thrb.v16i2.7","article-title":"Leptospira infections in freshwater fish in Morogoro Tanzania: A hidden public health threat","volume":"16","author":"Mgode","year":"2014","journal-title":"Tanzan. J. Health Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Assenga, J.A., Matemba, L.E., Muller, S.K., Mhamphi, G.G., and Kazwala, R.R. (2015). Predominant Leptospiral Serogroups Circulating among Humans, Livestock and Wildlife in Katavi-Rukwa Ecosystem, Tanzania. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003607"},{"key":"ref_13","unstructured":"Yanda, P.Z., Majule, A.E., and Mwakaje, A.G. (2004, January 25\u201326). Wetlands utilization, poverty alleviation and environmental conservation in semi-arid areas of Tanzania: The case of Singida and Dodoma regions. Proceedings of the REPOA 9th Annual Workshop, White Sands Hotel, Dar es Salaam, Tanzania."},{"key":"ref_14","unstructured":"Machang\u2019u, R. (2006, January 3\u20136). Rodent and human disease in Tanzania. Proceedings of the RatZooMan Workshop, Malelane, South Africa. Available online: http:\/\/projects.nri.org\/ratzooman\/docs\/workshop_proceedings.pdf."},{"key":"ref_15","unstructured":"Council, B.D. (2010). District Socio-Economic Profile 2008, Bahi District Council."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.rvsc.2015.01.014","article-title":"Antibodies to Leptospira interrogans in goats and risk factors of the disease in Santa Catarina (West side), Brazil","volume":"99","author":"Topazio","year":"2015","journal-title":"Res. Veter. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1476-0711-5-28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species","volume":"5","author":"Ahmed","year":"2006","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1128\/am.25.6.976-980.1973","article-title":"Improved microtechnique for the Leptospiral agglutination test","volume":"25","author":"Cole","year":"1973","journal-title":"Appl. Microbiol."},{"key":"ref_19","first-page":"12E.5.1","article-title":"Leptospirosis serodiagnosis by the microscopic agglutination test","volume":"32","author":"Goris","year":"2013","journal-title":"Curr. Prot. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.1002\/sim.2832","article-title":"Chi-squared and Fisher\u2013Irwin tests of two-by-two tables with small sample recommendations","volume":"26","author":"Campbell","year":"2007","journal-title":"Stat. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/sim.4116","article-title":"The analysis of 2 \u00d7 2 contingency tables\u2014Yet again","volume":"30","author":"Richardson","year":"2011","journal-title":"Stat. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1111\/j.1476-5381.2010.00873.x","article-title":"Guidelines for reporting experiments involving animals: The ARRIVE guidelines","volume":"160","author":"McGrath","year":"2010","journal-title":"Brit. J. Pharmacol."},{"key":"ref_23","unstructured":"Mgode, G.F., Mhamphi, G.G., Katakweba, A.S., Mboera, L.E.G., and Machang\u2019u, R.S. (2016). Leptospirosis in Tanzania: A Neglected Cause of Febrile Illness that Needs Attention of the Health System, NIMR Headquarter. Policy Brief no. SUA-SPMC\/PB\/001\/2016; 2016; Policy brief dialogue: Setting Health Policy Agenda for 2016 and Beyond."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9212","DOI":"10.1038\/s41598-018-27555-2","article-title":"Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease","volume":"8","author":"Esteves","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1128\/JCM.02452-10","article-title":"Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences","volume":"49","author":"Bourhy","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mgode, G.F., Japhary, M.M., Mhamphi, G.G., Kiwelu, I., Athaide, I., and Machang\u2019u, R.S. (2019). Leptospirosis in sugarcane plantation and fishing communities in Kagera northwestern Tanzania. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007225"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/696\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:10:35Z","timestamp":1760163035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,3]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["pathogens10060696"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10060696","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,3]]}}}