{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:03:26Z","timestamp":1760234606459,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T00:00:00Z","timestamp":1622937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Aspergillosis is a life-threatening disease in patients with compromised immune systems. The process of fungal invasion is an important step during host cell infection. We investigated the transcription factor and promoter region of SFTPD, which is activated during the infection process in conidia-treated cells. To investigate the promoter activity of SFTPD in fungal-infected cells, we cloned various lengths of the promoter region (\u22121000 to +1) of SFTPD and examined its activity in A549 cells treated with Aspergillus fumigatus conidia. We determined the location within the promoter region of SFTPD that exhibits a response to conidia infection. AliBaba 2.1 software was used to predict the transcription factor involved as well as the binding sites in the SFTPD promoter region. The results of a decoy assay show that the HSF1 transcription factor is sufficient to decrease the SFTPD expression. Using chromatin immunoprecipitation, we confirmed that HSF1 directly binds to the selected sequence, which is located in the response region (\u2212142 to \u2212134 bp). These findings suggest that inhibiting the binding of HSF1 to the promoter region of SFTPD is an important step to prevent conidia infection.<\/jats:p>","DOI":"10.3390\/pathogens10060709","type":"journal-article","created":{"date-parts":[[2021,6,7]],"date-time":"2021-06-07T01:56:40Z","timestamp":1623031000000},"page":"709","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcription Factor HSF1 Suppresses the Expression of Surfactant Protein D in Cells Infected with Aspergillus fumigatus"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7908-423X","authenticated-orcid":false,"given":"Sung-Su","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biomedical Laboratory Science, Daejeon University, Daejeon 34520, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7762-9593","authenticated-orcid":false,"given":"Kwang-Soo","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School, Daejeon University, Daejeon 34520, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s00277-017-3196-2","article-title":"Primary prophylaxis of invasive fungal infections in patients with haematological malignancies: 2017 update of the recommendations of the Infectious Diseases Working Party (AGIHO) of the German Society for Haematology and Medical Oncology (DGHO)","volume":"97","author":"Mellinghoff","year":"2018","journal-title":"Ann. Hematol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/bjh.16452","article-title":"Fungal infections in children with haematologic malignancies and stem cell transplant recipients","volume":"189","author":"Otto","year":"2020","journal-title":"Br. J. Haematol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1086\/505870","article-title":"Comparison of epidemiological, clinical, and biological features of invasive aspergillosis in neutropenic and nonneutropenic patients: A 6-year survey","volume":"43","author":"Cornillet","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","first-page":"R191","article-title":"Isolation of Aspergillus spp. from the respiratory tract in critically ill patients: Risk factors, clinical presentation and outcome","volume":"9","author":"Leon","year":"2005","journal-title":"Crit. Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s15010-013-0539-3","article-title":"SIMIFF study: Italian fungal registry of mold infections in hematological and non-hematological patients","volume":"42","author":"Montagna","year":"2014","journal-title":"Infection"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/S2213-2600(18)30274-1","article-title":"Invasive aspergillosis in patients admitted to the intensive care unit with severe influenza: A retrospective cohort study","volume":"6","author":"Schauwvlieghe","year":"2018","journal-title":"Lancet Respir. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1056\/NEJMra0808853","article-title":"Aspergillosis","volume":"360","author":"Segal","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Sharon, H., Amar, D., Levdansky, E., Mircus, G., Shadkchan, Y., Shamir, R., and Osherov, N. (2011). PrtT-regulated proteins secreted by Aspergillus fumigatus activate MAPK signaling in exposed A549 lung cells leading to necrotic cell death. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017509"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/nri1528","article-title":"Immunoregulatory functions of surfactant proteins","volume":"5","author":"Wright","year":"2005","journal-title":"Nat. Rev. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1165\/ajrcmb.19.4.3254","article-title":"Surfactant protein-A-deficient mice are susceptible to Pseudomonas aeruginosa infection","volume":"19","author":"LeVine","year":"1998","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"36250","DOI":"10.1074\/jbc.M303086200","article-title":"Macrophage-independent fungicidal action of the pulmonary collectins","volume":"278","author":"McCormack","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1177\/44.10.8813084","article-title":"Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung","volume":"44","author":"Zhou","year":"1996","journal-title":"J. Histochem. Cytochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1146\/annurev.ge.22.120188.003215","article-title":"The heat-shock proteins","volume":"22","author":"Lindquist","year":"1988","journal-title":"Annu. Rev. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nrm2938","article-title":"Heat shock factors: Integrators of cell stress, development and lifespan","volume":"11","author":"Akerfelt","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.molcel.2010.10.006","article-title":"The heat shock response: Life on the verge of death","volume":"40","author":"Richter","year":"2010","journal-title":"Mol. Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1007\/s00018-018-2836-6","article-title":"Roles of heat shock factor 1 beyond the heat shock response","volume":"75","author":"Barna","year":"2018","journal-title":"Cell Mol. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.tcb.2017.08.002","article-title":"Rethinking HSF1 in Stress, Development, and Organismal Health","volume":"27","author":"Li","year":"2017","journal-title":"Trends Cell Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3788","DOI":"10.1101\/gad.12.24.3788","article-title":"Regulation of the heat shock transcriptional response: Cross talk between a family of heat shock factors, molecular chaperones, and negative regulators","volume":"12","author":"Morimoto","year":"1998","journal-title":"Genes Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e00140-18","DOI":"10.1128\/CMR.00140-18","article-title":"Aspergillus fumigatus and Aspergillosis in 2019","volume":"33","author":"Latge","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.jinf.2010.06.018","article-title":"Trends in the postmortem epidemiology of invasive fungal infections at a university hospital","volume":"61","author":"Lehrnbecher","year":"2010","journal-title":"J. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s00277-002-0599-4","article-title":"Clinical applicability of the new EORTC\/MSG classification for invasive pulmonary aspergillosis in patients with hematological malignancies and autopsy-confirmed invasive aspergillosis","volume":"82","author":"Subira","year":"2003","journal-title":"Ann. Hematol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1183\/09059180.00001011","article-title":"Pulmonary aspergillosis: A clinical review","volume":"20","author":"Kousha","year":"2011","journal-title":"Eur. Respir. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/s13054-015-1140-1","article-title":"Putative invasive pulmonary aspergillosis in critically ill patients with chronic obstructive pulmonary disease: A matched cohort study","volume":"19","author":"Delsuc","year":"2015","journal-title":"Crit. Care"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1093\/mmy\/myw114","article-title":"Invasive aspergillosis in acute myeloid leukemia: Are we making progress in reducing mortality?","volume":"55","author":"Dragonetti","year":"2017","journal-title":"Med. Mycol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"165rv113","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: Human fungal infections","volume":"4","author":"Brown","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S14","DOI":"10.1016\/j.amjmed.2011.10.008","article-title":"Invasive mycoses: Diagnostic challenges","volume":"125","year":"2012","journal-title":"Am. J. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1378\/chest.118.5.1506","article-title":"Mushroom worker\u2019s lung caused by spores of Hypsizigus marmoreus (Bunashimeji): Elevated serum surfactant protein D levels","volume":"118","author":"Tanaka","year":"2000","journal-title":"Chest"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1164\/ajrccm.162.1.9903014","article-title":"Serum levels of surfactant proteins A and D are useful biomarkers for interstitial lung disease in patients with progressive systemic sclerosis","volume":"162","author":"Takahashi","year":"2000","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1172\/JCI16889","article-title":"Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability","volume":"111","author":"Wu","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8657","DOI":"10.1021\/bi060176z","article-title":"Human pulmonary surfactant protein D binds the extracellular domains of Toll-like receptors 2 and 4 through the carbohydrate recognition domain by a mechanism different from its binding to phosphatidylinositol and lipopolysaccharide","volume":"45","author":"Ohya","year":"2006","journal-title":"Biochemistry"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"S155","DOI":"10.1080\/13693780500088408","article-title":"Role of collectins in innate immunity against aspergillosis","volume":"43","author":"Madan","year":"2005","journal-title":"Med. Mycol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4901","DOI":"10.1074\/jbc.M117.815852","article-title":"Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response to Aspergillus fumigatus spores","volume":"293","author":"Wong","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.1128\/iai.65.8.3171-3179.1997","article-title":"Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages","volume":"65","author":"Madan","year":"1997","journal-title":"Infect. Immun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1165\/rcmb.2005-0461OC","article-title":"Surfactant proteins A and D enhance pulmonary clearance of Pseudomonas aeruginosa","volume":"34","author":"Giannoni","year":"2006","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1165\/ajrcmb.25.1.4391","article-title":"Aspergillus fumigatus-induced allergic airway inflammation alters surfactant homeostasis and lung function in BALB\/c mice","volume":"25","author":"Haczku","year":"2001","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"34578","DOI":"10.1074\/jbc.M404296200","article-title":"Nuclear factor of activated T cells regulates transcription of the surfactant protein D gene (Sftpd) via direct interaction with thyroid transcription factor-1 in lung epithelial cells","volume":"279","author":"Dave","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"19530","DOI":"10.1074\/jbc.M201126200","article-title":"Surfactant protein D gene regulation. Interactions among the conserved CCAAT\/enhancer-binding protein elements","volume":"277","author":"He","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"31051","DOI":"10.1074\/jbc.M003499200","article-title":"Proximal promoter of the surfactant protein D gene: Regulatory roles of AP-1, forkhead box, and GT box binding proteins","volume":"275","author":"He","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cellimm.2018.01.015","article-title":"Heat shock factor 1 suppresses the HIV-induced inflammatory response by inhibiting nuclear factor-kappaB","volume":"327","author":"Pan","year":"2018","journal-title":"Cell Immunol."},{"key":"ref_40","first-page":"1392","article-title":"Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress","volume":"13","author":"Sarge","year":"1993","journal-title":"Mol. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.tcb.2015.10.011","article-title":"HSF1: Guardian of Proteostasis in Cancer","volume":"26","author":"Dai","year":"2016","journal-title":"Trends Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1042\/BCJ20160005","article-title":"Mechanisms of Hsp90 regulation","volume":"473","author":"Prodromou","year":"2016","journal-title":"Biochem. J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/labinvest.3700749","article-title":"Coexpression of myofibroblast and macrophage markers: Novel evidence for an in vivo plasticity of chorioamniotic mesodermal cells of the human placenta","volume":"88","author":"Kim","year":"2008","journal-title":"Lab. Investig."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/709\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:11:26Z","timestamp":1760163086000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,6]]},"references-count":44,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["pathogens10060709"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10060709","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2021,6,6]]}}}