{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T20:35:37Z","timestamp":1788813337183,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Currently, the diagnosis of Lyme disease is based mostly on two-tiered serologic testing. In the new generation of immunoenzymatic assays, antigens comprise whole-cell lysates of members of the Borrelia burgdorferi sensu lato (s.l.) species complex, with the addition of selected recombinant proteins. Due to the high diversity of members of the B. burgdorferi s.l. genospecies and the low degree of conservation among the amino acid sequences of their proteins, serodiagnostic methods currently in use are not sufficient for the correct diagnosis of borreliosis. Two divalent chimeric proteins (BmpA-BBK32 and BmpA-BBA64) were expressed in Escherichia coli. Following purification by one-step metal-affinity chromatography, preparations were obtained containing milligram levels of chimeric protein exhibiting electrophoretic purity in excess of 98%. Reactivity of the new chimeric proteins with specific human IgG antibodies was preliminarily determined by Western blot. For this purpose, 20 negative sera and 20 positive sera was used. The new chimeric proteins were highly reactive with IgG antibodies contained in the serum of patients suffering from borreliosis. Moreover, no immunoreactivity of chimeric proteins was observed with antibodies in the sera of healthy people. These promising results suggest that new chimeric proteins have the potential to discriminate between positive and negative sera.<\/jats:p>","DOI":"10.3390\/pathogens10060767","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T04:10:47Z","timestamp":1623989447000},"page":"767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Borrelia burgdorferi BmpA-BBK32 and BmpA-BBA64: New Recombinant Chimeric Proteins with Potential Diagnostic Value"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6420-4721","authenticated-orcid":false,"given":"Weronika","family":"Gr\u0105\u017alewska","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2176-3966","authenticated-orcid":false,"given":"Bart\u0142omiej","family":"Ferra","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Rudzi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Epidemiology, Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5421-6905","authenticated-orcid":false,"given":"Lucyna","family":"Holec-G\u0105sior","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1080\/10408363.2018.1450353","article-title":"Laboratory diagnosis of Lyme borreliosis: Current state of the art and future perspectives","volume":"55","author":"Lohr","year":"2018","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1469-0691.2011.03492.x","article-title":"The expanding Lyme Borrelia complex-clinical significance of genomic species?","volume":"17","author":"Stanek","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_3","first-page":"4757","article-title":"Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States","volume":"123","author":"Pritt","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13071-016-1353-4","article-title":"A divergent spirochete strain isolated from a resident of the southeastern United States was identified by multilocus sequence typing as Borrelia bissettii","volume":"9","author":"Golovchenko","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1046\/j.1365-2958.2000.01698.x","article-title":"A bacterial genome in flux: The twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi","volume":"35","author":"Casjens","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Casjens, S.R., Mongodin, E.F., Qiu, W.G., Luft, B.J., Schutzer, S.E., Gilcrease, E.B., Huang, W.M., Vujadinovic, M., Aron, J.K., and Vargas, L.C. (2012). Genome stability of lyme disease spirochetes: Comparative genomics of Borrelia burgdorferi plasmids. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033280"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6038","DOI":"10.1093\/nar\/gkh953","article-title":"Comparative analysis of the Borrelia garinii genome","volume":"32","author":"Lehmann","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-017-3553-5","article-title":"Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi","volume":"18","author":"Casjens","year":"2017","journal-title":"BMC Genom."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"13865","DOI":"10.1073\/pnas.97.25.13865","article-title":"Correlation between plasmid content and infectivity in Borrelia burgdorferi","volume":"97","author":"Purser","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2909","DOI":"10.1073\/pnas.92.7.2909","article-title":"Induction of an outer surface protein on Borrelia burgdorferi during tick feeding","volume":"92","author":"Schwan","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1128\/IAI.71.4.1689-1705.2003","article-title":"Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays","volume":"71","author":"Ojaimi","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-695X.2012.00980.x","article-title":"The role of Borrelia burgdorferi outer surface proteins","volume":"66","author":"Kenedy","year":"2012","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.ijmm.2006.01.006","article-title":"Molecular analysis of decorin-binding protein A (DbpA) reveals five major groups among European Borrelia burgdorferi sensu lato strains with impact for the development of serological assays and indicates lateral gene transfer of the dbpA gene","volume":"296","author":"Fingerle","year":"2006","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_14","first-page":"909","article-title":"Comprehensive seroprofiling of sixteen B. burgdorferi OspC: Implications for Lyme disease diagnostics design","volume":"35","author":"Ivanova","year":"2011","journal-title":"Clin. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/37551","article-title":"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi","volume":"390","author":"Fraser","year":"1997","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7515","DOI":"10.1073\/pnas.1000268107","article-title":"The bba64 gene of Borrelia burgdorferi, the Lyme disease agent, is critical for mammalian infection via tick bite transmission","volume":"107","author":"Gilmore","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.1128\/IAI.01950-05","article-title":"Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection","volume":"74","author":"Tilly","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1080\/14737159.2016.1246959","article-title":"Progress in the molecular diagnosis of Lyme disease","volume":"17","author":"Cerar","year":"2017","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1128\/CMR.18.3.484-509.2005","article-title":"Diagnosis of Lyme Borreliosis","volume":"18","author":"Wang","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1128\/jcm.31.12.3090-3095.1993","article-title":"Serodiagnosis in early Lyme disease","volume":"31","author":"Nowakowski","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1128\/jcm.33.2.419-427.1995","article-title":"Immunoblot interpretation criteria for serodiagnosis of early Lyme disease","volume":"33","author":"Engstrom","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","first-page":"1","article-title":"Cutaneous Lyme borreliosis: Guideline of the German Dermatology Society","volume":"15","author":"Hofmann","year":"2017","journal-title":"Ger. Med. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1128\/JCM.03394-15","article-title":"The Past, Present, and (Possible) Future of Serologic Testing for Lyme Disease","volume":"54","author":"Theel","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.1128\/JCM.43.8.3602-3609.2005","article-title":"Improvement of Lyme Borreliosis serodiagnosis by a newly developed recombinant immunoglobulin G (IgG) and IgM line immunoblot assay and addition of VlsE and DbpA homologues","volume":"43","author":"Goettner","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1128\/CDLI.12.11.1269-1274.2005","article-title":"Evaluation of a Western blot method for the detection of Yersinia antibodies: Evidence of serological cross-reactivity between Yersinia outer membrane proteins and Borrelia burgdorferi","volume":"12","author":"Rawlins","year":"2005","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/jcm.34.2.237-240.1996","article-title":"Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis","volume":"34","author":"Magnarelli","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/BF02098084","article-title":"Cross-reactive proteins of Borrelia burgdorferi","volume":"11","author":"Bruckbauer","year":"1992","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5275","DOI":"10.1128\/IAI.66.11.5275-5285.1998","article-title":"Molecular analysis of sequence heterogeneity among genes encoding decorin binding proteins A and B of Borrelia burgdorferi sensu lato","volume":"66","author":"Roberts","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.1128\/jcm.31.10.2570-2576.1993","article-title":"Polymorphism in ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: Implications for taxonomy and for use of OspC protein as a diagnostic antigen","volume":"31","author":"Theisen","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S1286-4579(01)01435-6","article-title":"Analysis of Borrelia burgdorferi gene expression during life cycle phases of the tick vector Ixodes scapularis","volume":"3","author":"Gilmore","year":"2001","journal-title":"Microbes Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1128\/jcm.33.9.2260-2264.1995","article-title":"Antibodies against whole sonicated Borrelia burgdorferi spirochetes, 41- kilodalton flagellin, and P39 protein in patients with PCR- or culture- proven late Lyme borreliosis","volume":"33","author":"Oksi","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1128\/JCM.40.4.1174-1180.2002","article-title":"Recombinant BBK32 protein in serodiagnosis of early and late Lyme borreliosis","volume":"40","author":"Panelius","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1128\/JCM.36.12.3474-3479.1998","article-title":"Peptide-Based OspC Enzyme-Linked Immunosorbent Assay for serodiagnosis of Lyme Borreliosis","volume":"36","author":"Mathiesen","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1007\/s00415-003-0205-2","article-title":"Diagnosis of Lyme neuroborreliosis with antibodies to recombinant proteins DbpA, BBK32, and OspC, and VIsE IR6 peptide","volume":"250","author":"Panelius","year":"2003","journal-title":"J. Neurol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/jcm.35.1.86-91.1997","article-title":"Molecular characterization of a 35-kilodalton protein of Borrelia burgdorferi, an antigen of diagnostic importance in early Lyme disease","volume":"35","author":"Gilmore","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exppara.2007.12.002","article-title":"Toxoplasma gondii: Enzyme-linked immunosorbent assay using different fragments of recombinant microneme protein 1 (MIC1) for detection of immunoglobulin G antibodies","volume":"119","author":"Holec","year":"2008","journal-title":"Exp. Parasitol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.exppara.2009.10.010","article-title":"Toxoplasma gondii: Recombinant GRA5 antigen for detection of immunoglobulin G antibodies using enzyme-linked immunosorbent assay","volume":"124","author":"Kur","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1128\/CVI.00117-13","article-title":"Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: Current status of studies","volume":"20","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1128\/JCM.36.4.857-861.1998","article-title":"Enzyme-Linked Immunosorbent Assay using recombinant OspC and the internal 14-kDa flagellin fragment for serodiagnosis of early Lyme disease","volume":"36","author":"Rauer","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Magalh\u00e3es, F.B., Castro Neto, A.L., Nascimento, M.B., Santos, W.J.T., Medeiros, Z.M., Neto, A.S.L., Costa, D.L., Costa, C.H.N., Dos Santos, W.L.C., and Pontes de Carvalho, L.C. (2017). Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0184867"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ferra, B.T., Holec-G\u0105sior, L., Gatkowska, J., Dziadek, B., Dzitko, K., Gr\u0105\u017alewska, W., and Lautenbach, D. (2019). The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0217866"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1128\/CVI.00452-12","article-title":"MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis","volume":"19","author":"Ferra","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1128\/CVI.05433-11","article-title":"A new MIC1-MAG1 recombinant chimeric antigen can be used instead of the Toxoplasma gondii lysate antigen in serodiagnosis of human toxoplasmosis","volume":"19","author":"Ferra","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.diagmicrobio.2015.01.018","article-title":"New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis","volume":"82","author":"Kur","year":"2015","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1007\/s00436-015-4552-6","article-title":"A new Toxoplasma gondii chimeric antigen containing fragments of SAG2, GRA1, and ROP1 proteins\u2014impact of immunodominant sequences size on its diagnostic usefulness","volume":"114","author":"Ferra","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Del-Rei, R.P., Leony, L.M., Celedon, P.A.F., Zanchin, N.I.T., dos Reis, M.G., de Miranda Gomes, Y., Schijman, A.G., Longhi, S.A., and Santos, F.L.N. (2019). Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0215623"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Santos, W.J.T., Tavares, D.H.C., Castro Neto, A.L., Nascimento, M.B., Dhalia, R., Albuquerque, A.L., Costa, C.H.N., Magalh\u00e3es, F.B., Rezende, A.M., and Neto, O.P.M. (2020). Gene design, optimization of protein expression and preliminary evaluation of a new chimeric protein for the serological diagnosis of both human and canine visceral leishmaniasis. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008488"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1002\/pro.3125","article-title":"Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold","volume":"26","author":"Norek","year":"2017","journal-title":"Protein Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1128\/CVI.00608-12","article-title":"Outer surface protein C peptide derived from Borrelia burgdorferi sensu stricto as a target for serodiagnosis of early Lyme disease","volume":"20","author":"Arnaboldi","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_50","first-page":"119","article-title":"Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes","volume":"66","year":"2019","journal-title":"Acta Biochim. Pol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1128\/IAI.68.4.2349-2352.2000","article-title":"Epitope mapping of the immunodominant invariable region of Borrelia burgdorferi VlsE in three host species","volume":"68","author":"Liang","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.1128\/JCM.38.7.2530-2535.2000","article-title":"Recombinant Chimeric Borrelia proteins for diagnosis of Lyme disease","volume":"38","author":"Dunn","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1001\/archinte.161.16.2015","article-title":"A first-tier rapid assay for the serodiagnosis of Borrelia burgdorferi infection","volume":"161","author":"Wormser","year":"2001","journal-title":"Arch. Intern. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3046","DOI":"10.1128\/JCM.01004-17","article-title":"Improved serodiagnostic performance for Lyme disease by use of two recombinant proteins in ELISA as compared to standardized two tier testing","volume":"55","author":"Bradshaw","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1016\/j.celrep.2016.08.013","article-title":"Biomechanics of Borrelia burgdorferi Vascular Interactions","volume":"16","author":"Ebady","year":"2016","journal-title":"Cell Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1128\/IAI.74.1.435-441.2006","article-title":"Fibronectin binding protein BBK32 of the Lyme disease spirochete promotes bacterial attachment to glycosaminoglycans","volume":"74","author":"Fischer","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4940","DOI":"10.1128\/IAI.01420-08","article-title":"Borrelia burgdorferi BmpA is a laminin-binding protein","volume":"77","author":"Verma","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2752","DOI":"10.1128\/jcm.35.11.2752-2758.1997","article-title":"Heterogeneity of BmpA (P39) among European isolates of Borrelia burgdorferi sensu lato and influence of interspecies variability on serodiagnosis","volume":"35","author":"Roessler","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1128\/CVI.00824-13","article-title":"Evaluation of the Borrelia burgdorferi BBA64 protein as a protective immunogen in mice","volume":"21","author":"Brandt","year":"2014","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1006\/prep.1999.1044","article-title":"Cloning, overexpression, and purification of the recombinant His-tagged SSB protein of Escherichia coli and use in polymerase chain reaction amplification","volume":"16","author":"Kur","year":"1999","journal-title":"Protein Expr. Purif."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/767\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:18:00Z","timestamp":1760163480000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/6\/767"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,18]]},"references-count":60,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["pathogens10060767"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10060767","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,18]]}}}