{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:55:12Z","timestamp":1791352512688,"version":"4.3.1"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303999\/2016-0"],"award-info":[{"award-number":["303999\/2016-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["440405\/2016-5"],"award-info":[{"award-number":["440405\/2016-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011893","name":"Pan American Health Organization","doi-asserted-by":"publisher","award":["SCON2016-01353"],"award-info":[{"award-number":["SCON2016-01353"]}],"id":[{"id":"10.13039\/100011893","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI142759"],"award-info":[{"award-number":["AI142759"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI127744"],"award-info":[{"award-number":["AI127744"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI136126"],"award-info":[{"award-number":["AI136126"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Reporter virus neutralization test (RVNT) has been used as an alternative to the more laborious and time-demanding conventional PRNT assay for both DENV and ZIKV. However, few studies have investigated how these techniques would perform in epidemic areas with the circulation of multiple flavivirus. Here, we evaluate the performance of ZIKV and DENV Rluc RVNT and ZIKV mCh RVNT assays in comparison to the conventional PRNT assay against patient sera collected before and during ZIKV outbreak in Brazil. These samples were categorized into groups based on (1) acute and convalescent samples according to the time of disease, and (2) laboratorial diagnostic results (DENV and ZIKV RT-PCR and IgM-capture ELISA). Our results showed that DENV Rluc assay presented 100% and 78.3% sensitivity and specificity, respectively, with 93.3% accuracy, a similar performance to the traditional PRNT. ZIKV RVNT90, on the other hand, showed much better ZIKV antibody detection performance (around nine-fold higher) when compared to PRNT, with 88% clinical sensitivity. Specificity values were on average 76.8%. Even with these results, however, ZIKV RVNT90 alone was not able to reach a final diagnostic conclusion for secondary infection in human samples due to flavivirus cross reaction. As such, in regions where the flavivirus differential diagnosis represents a challenge, we suggest the establishment of a RVNT panel including other flaviviruses circulating in the region, associated with the other serological techniques such as IgM ELISA and the investigation of seroconversion, in order to help define an accurate diagnostic conclusion using serology.<\/jats:p>","DOI":"10.3390\/pathogens10070840","type":"journal-article","created":{"date-parts":[[2021,7,4]],"date-time":"2021-07-04T22:34:36Z","timestamp":1625438076000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Reporter Virus Neutralization Test Evaluation for Dengue and Zika Virus Diagnosis in Flavivirus Endemic Area"],"prefix":"10.3390","volume":"10","author":[{"given":"Jannyce G. C.","family":"Nunes","sequence":"first","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77550, USA"},{"name":"Post Graduation Program in Parasitary Biology in the Amazon, Bel\u00e9m 66050-540, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-9665","authenticated-orcid":false,"given":"Bruno T. D.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77550, USA"},{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-3782","authenticated-orcid":false,"given":"Chao","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77550, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana F.","family":"Moraes","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tais R.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria H. R.","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane L.","family":"das Chagas","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco A. e","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1932-9976","authenticated-orcid":false,"given":"Raimunda S. S.","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana V. P.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livia C.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannifer O.","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8578-9984","authenticated-orcid":false,"given":"Livia M. N.","family":"Casseb","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele F.","family":"Henriques","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro F. C.","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Department of Arbovirology & Hemorrhagic Fever, Evandro Chagas Institute, Ananindeua 67015-120, PA, Brazil"},{"name":"Science and Health Institute, Par\u00e1 State University, Bel\u00e9m 66113-010, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4872-234X","authenticated-orcid":false,"given":"Rommel M. R.","family":"Burbano","sequence":"additional","affiliation":[{"name":"Biological Sciences Institute, ICS, Federal University of Par\u00e1, Bel\u00e9m 66050-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77550, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele B. 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