{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T19:46:14Z","timestamp":1777751174834,"version":"3.51.4"},"reference-count":62,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T00:00:00Z","timestamp":1625529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014417","name":"Pa\u0144stwowy Instytut Weterynaryjny - Pa\u0144stwowy Instytut Badawczy","doi-asserted-by":"publisher","award":["S\/327"],"award-info":[{"award-number":["S\/327"]}],"id":[{"id":"10.13039\/501100014417","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04% to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time.<\/jats:p>","DOI":"10.3390\/pathogens10070853","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T11:36:44Z","timestamp":1625571404000},"page":"853","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus)"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5830-167X","authenticated-orcid":false,"given":"Jacek","family":"Karamon","sequence":"first","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2135-701X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Samorek-Pier\u00f3g","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacek","family":"Sroka","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Bilska-Zaj\u0105c","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"D\u0105browska","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-3028","authenticated-orcid":false,"given":"Maciej","family":"Kochanowski","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8125-973X","authenticated-orcid":false,"given":"Miros\u0142aw","family":"R\u00f3\u017cycki","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7297-2082","authenticated-orcid":false,"given":"Jolanta","family":"Zdybel","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3649-7867","authenticated-orcid":false,"given":"Tomasz","family":"Cencek","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Pu\u0142awy, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/bs.apar.2016.11.002","article-title":"Chapter Five\u2014Ecology and Life Cycle Patterns of Echinococcus Species","volume":"Volume 95","author":"Pt","year":"2017","journal-title":"Advances in Parasitology, Echinococcus and Echinococcosis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/S1471-4922(02)02358-9","article-title":"Towards a taxonomic revision of the genus Echinococcus","volume":"18","author":"Thompson","year":"2002","journal-title":"Trends Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/bs.apar.2016.07.002","article-title":"Chapter three-Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus","volume":"Volume 95","author":"Pt","year":"2017","journal-title":"Advances in Parasitology, Echinococcus and Echinococcosis"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.7589\/0090-3558-45.4.1208","article-title":"Echinococcus granulosus in Gray Wolves and Ungulates in Idaho and Montana, USA","volume":"45","author":"Foreyt","year":"2009","journal-title":"J. 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