{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T18:17:57Z","timestamp":1777486677457,"version":"3.51.4"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011091","name":"FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu","doi-asserted-by":"publisher","award":["RF-10\/6234"],"award-info":[{"award-number":["RF-10\/6234"]}],"id":[{"id":"10.13039\/501100011091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Chlamydia suis (C. suis) resides in the intestines of pigs and tetracycline-resistant strains are emerging worldwide. Intestinal infections are often subclinical. However, the gut is regarded as a C. suis reservoir and clinical infections have been associated with enteritis, conjunctivitis, pneumonia and reproductive failure. C. suis was found in boar semen and venereal transmission occurred. We studied the anti-Chlamydia suis activity of ovotransferrin (ovoTF) and bovine lactoferrin (bLF). Pre-incubation of C. suis with bLF or ovoTF had no significant effect on overall chlamydia replication (mean fluorescence area) in McCoy cells. The addition of ovoTF to the culture medium had no effect on bacterial replication, but the addition of 0.5 or 5 mg\/mL of bLF significantly reduced the inclusion size by 17% and 15% respectively. Egg components are used for cryopreservation of boar semen. When inoculating an ovoTF-containing and Chlamydia suis-spiked semen sample in McCoy cells, a significant reduction in inclusion number (by 7%) and overall replication (by 11%) was observed. Thus, we showed that transferrins possess anti-chlamydial activity. Moreover, ovoTF addition to semen extenders might reduce C. suis venereal transmission. Further research is needed to unravel the mechanisms behind the observations and to enhance the effect of transferrins on C. suis.<\/jats:p>","DOI":"10.3390\/pathogens10070858","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T12:31:25Z","timestamp":1625661085000},"page":"858","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transferrins Reduce Replication of Chlamydia suis in McCoy Cells"],"prefix":"10.3390","volume":"10","author":[{"given":"Leentje","family":"De Puysseleyr","sequence":"first","affiliation":[{"name":"Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristien","family":"De Puysseleyr","sequence":"additional","affiliation":[{"name":"Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Rybarczyk","sequence":"additional","affiliation":[{"name":"Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3425-1345","authenticated-orcid":false,"given":"Paulien","family":"Vander Donck","sequence":"additional","affiliation":[{"name":"Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winnok H.","family":"De Vos","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology and Histology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Vanrompay","sequence":"additional","affiliation":[{"name":"Laboratory for Immunology and Animal Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"ftaa040","DOI":"10.1093\/femspd\/ftaa040","article-title":"Chlamydia suis Is Associated with Intestinal NF-\u039aB Activation in Experimentally Infected Gnotobiotic Piglets","volume":"78","author":"Aumayer","year":"2020","journal-title":"Pathog. 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