{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:14:41Z","timestamp":1773288881544,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>If validated beforehand, the analysis of dried blood on blotting paper (BP samples) is very useful for monitoring free-ranging animals. We aimed to validate this method for the detection of antibodies against Toxoplasma gondii in stray cats. We used the modified agglutination test (MAT) in 199 sample pairs of sera and BP samples from 54, 39, 56, and 50 cats trapped during four periods in five dairy farms. Screening was at 1:6, 1:12, and 1:24 dilutions. The cut-off value was at MAT titre \u2265 24, but MAT titre \u2265 12 was also considered for BP samples that often have a higher dilution level. Depending on the period, sample type, and cut-off value, sensitivity of the analysis of the BP sample vs. serum varied from 87.1% to 100% and specificity ranged from 72.22% to 100%. The concordance values and Kappa coefficient showed a substantial to excellent agreement between the results of the two methods, whatever the cut-off value. These findings quantifiably validate the use of MAT on BP samples for the detection of antibodies to T. gondii in stray cats, but we recommend expressing results from BP samples with several cut-off values as the MAT titres tend to be lower than those of sera.<\/jats:p>","DOI":"10.3390\/pathogens10070864","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T10:42:17Z","timestamp":1625740937000},"page":"864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Validation of the Use of Dried Blood Samples for the Detection of Toxoplasma gondii Antibodies in Stray Cats (Felis s. catus)"],"prefix":"10.3390","volume":"10","author":[{"given":"Julie Alice","family":"Simon","sequence":"first","affiliation":[{"name":"Epid\u00e9mio-Surveillance et Circulation des Parasites dans les Environnements (ESCAPE), EA 7510, CAP SANTE, Universit\u00e9 de Reims Champagne Ardenne, CEDEX, 51095 Reims, France"},{"name":"Centre de Recherche et de Formation en Eco-\u00e9thologie (CERFE), Universit\u00e9 de Reims Champagne-Ardenne, 08240 Boult-aux-Bois, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Aubert","sequence":"additional","affiliation":[{"name":"Epid\u00e9mio-Surveillance et Circulation des Parasites dans les Environnements (ESCAPE), EA 7510, CAP SANTE, Universit\u00e9 de Reims Champagne Ardenne, CEDEX, 51095 Reims, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Centre de Ressources Biologiques <i>Toxoplasma<\/i>, CHU Reims, CEDEX, 51092 Reims, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9gine","family":"Geers","sequence":"additional","affiliation":[{"name":"Epid\u00e9mio-Surveillance et Circulation des Parasites dans les Environnements (ESCAPE), EA 7510, CAP SANTE, Universit\u00e9 de Reims Champagne Ardenne, CEDEX, 51095 Reims, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Centre de Ressources Biologiques <i>Toxoplasma<\/i>, CHU Reims, CEDEX, 51092 Reims, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[{"name":"Epid\u00e9mio-Surveillance et Circulation des Parasites dans les Environnements (ESCAPE), EA 7510, CAP SANTE, Universit\u00e9 de Reims Champagne Ardenne, CEDEX, 51095 Reims, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Centre de Ressources Biologiques <i>Toxoplasma<\/i>, CHU Reims, CEDEX, 51092 Reims, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Lazarine","family":"Poulle","sequence":"additional","affiliation":[{"name":"Epid\u00e9mio-Surveillance et Circulation des Parasites dans les Environnements (ESCAPE), EA 7510, CAP SANTE, Universit\u00e9 de Reims Champagne Ardenne, CEDEX, 51095 Reims, France"},{"name":"Centre de Recherche et de Formation en Eco-\u00e9thologie (CERFE), Universit\u00e9 de Reims Champagne-Ardenne, 08240 Boult-aux-Bois, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1631S","DOI":"10.1093\/jn\/131.5.1631S","article-title":"Use of filter paper for the collection and analysis of human whole blood specimens","volume":"131","author":"Mei","year":"2001","journal-title":"J. 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