{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:19:45Z","timestamp":1790662785351,"version":"4.1.0"},"reference-count":21,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:00:00Z","timestamp":1627603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Bordetella trematum is a relatively newly discovered and potentially frequently overlooked Bordetella species, mostly isolated from chronic wounds and predominantly in those of the lower extremities. Its susceptibility profile and clinical significance is still debated, given the limited amount of available data. We contribute providing a molecular and phenotypical analysis of three unique clinical B. trematum isolates detected between August 2019 and January 2020 to aid the matter. Cryo-conserved isolates were subcultured and re-identified using various routine means of identification. Bacterial genomes were fully Illumina-sequenced and phenotypical susceptibility was determined by broth microdilution and gradient-strip tests. All isolates displayed increased susceptibility to piperacillin\u2013tazobactam (&lt;2\/4 mg\/L), imipenem (&lt;1 mg\/L), and meropenem (&lt;0.047 mg\/L), whereas they displayed decreased susceptibility to all tested cephalosporins and fluoroquinolones (according to PK-PD, EUCAST 10.0 2020). One isolate carried a beta-lactamase (EC 3.5.2.6) and a sulfonamide resistance gene (sul2) and cells displayed resistance to ampicillin, ampicillin\/sulbactam, and trimethoprim\/sulfamethoxazole. All isolates carried genes conferring decreased susceptibility to aminoglycosides (aadA), fosfomycin (fosA) and fluoroquinolones (gyrB EC 5.99.1.3). Awareness that B. trematum can be resistant to trimethoprim\/sulfamethoxazole is warranted.<\/jats:p>","DOI":"10.3390\/pathogens10080966","type":"journal-article","created":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T21:44:32Z","timestamp":1627854272000},"page":"966","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection and Characterization of Clinical Bordetella trematum Isolates from Chronic Wounds"],"prefix":"10.3390","volume":"10","author":[{"given":"Christian","family":"Buechler","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2064-3885","authenticated-orcid":false,"given":"Claudio","family":"Neidh\u00f6fer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8985-0682","authenticated-orcid":false,"given":"Thorsten","family":"Hornung","sequence":"additional","affiliation":[{"name":"Clinic and Polyclinic for Dermatology and Allergology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Neuenhoff","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijo","family":"Par\u010dina","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1099\/00207713-46-4-849","article-title":"Bordetella trematum sp. nov., Isolated from Wounds and Ear Infections in Humans, and Reassessment of Alcaligenes denitrificans Ruger and Tan 1983","volume":"46","author":"Vandamme","year":"1996","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e000029","DOI":"10.1099\/jmmcr.0.000029","article-title":"Isolation of Bordetella species from unusual infection sites","volume":"2","author":"Almuzara","year":"2015","journal-title":"JMM Case Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1111\/j.1464-5491.2004.01310.x","article-title":"Isolation of Bordetella trematum from a diabetic leg ulcer","volume":"21","author":"Daxboeck","year":"2004","journal-title":"Diabet. Med."},{"key":"ref_4","first-page":"72","article-title":"Diabetic leg ulcer colonized by Bordetella trematum","volume":"26","author":"Cuervo","year":"2013","journal-title":"Rev. Esp. Quimioter."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.4103\/0974-777X.121996","article-title":"Nonfermenting Gram-negative Bacilli other than Pseudomonas aeruginosa and Acinetobacter spp. Causing Respiratory Tract Infections in a Tertiary Care Center","volume":"5","author":"Chawla","year":"2013","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_6","first-page":"612","article-title":"Isolement de Bordetella trematum au cours d\u2019une bacteriemie","volume":"72","author":"Halim","year":"2014","journal-title":"Ann. de Biol. Clin."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.diagmicrobio.2016.05.019","article-title":"Bordetella trematum sepsis with shock in a diabetic patient with rapidly developing soft tissue infection","volume":"8","author":"Majewski","year":"2016","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1007\/s15010-014-0717-y","article-title":"Bordetella trematum in chronic ulcers: Report on two cases and review of the literature","volume":"43","author":"Eder","year":"2015","journal-title":"Infection"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.medmal.2018.03.008","article-title":"Bact\u00e9ri\u00e9mie \u00e0 Bordetella trematum dans un contexte d\u2019immunosuppression","volume":"48","author":"Cattoen","year":"2018","journal-title":"M\u00e9d. Mal. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Castro, T.R.Y., Martins, R.C.R., Forno, N.L.F.D., Santana, L., Rossi, F., Schwarzbold, A., Costa, S.F., and Trindade, P.D.A. (2019). Bordetella trematum infection: Case report and review of previous cases. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4046-8"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e16","DOI":"10.1097\/IPC.0000000000000501","article-title":"Isolation of Xanthobacter species and Bordetella trematum in a patient with polymicrobial peritonitis","volume":"25","author":"Tena","year":"2017","journal-title":"Infect. Dis. Clin. Pract."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s12223-020-00784-7","article-title":"Isolation of Bordetella trematum from the respiratory tract of a patient with lung cancer: A case report","volume":"65","author":"Kukla","year":"2020","journal-title":"Folia Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"305","DOI":"10.4103\/0255-0857.154891","article-title":"Bordetella trematum bacteremia in an infant: A cause to look for","volume":"33","author":"Saksena","year":"2015","journal-title":"Indian J. Med. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"Res-Finder 4.0 for predictions of phenotypes from genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1093\/jac\/dkx217","article-title":"PointFinder: A novel web tool for WGS-based detection of antimicrobial resistance associate with chromosomal point mutations in bacterial pathogens","volume":"72","author":"Zankari","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST Server: Rapid Annotations using Subsystems Technology","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genom."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1093\/nar\/gkt1226","article-title":"The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)","volume":"42","author":"Overbeek","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8365","DOI":"10.1038\/srep08365","article-title":"RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes","volume":"5","author":"Brettin","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Davis, J.C., and Hunt, T.K. (1988). Tissue oxygen measurement. Problem Wounds: The Role of Oxygen, Elsevier.","DOI":"10.1097\/00006534-198909000-00030"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dalton, T., Dowd, S., Wolcott, R.D., Sun, Y., Watters, C., Griswold, J., and Rumbaugh, K.P. (2011). An in vivo polymicrobial biofilm wound infection model to study interspecies interactions. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027317"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1128\/CMR.14.2.244-269.2001","article-title":"Wound microbiology and associated approaches to wound management","volume":"14","author":"Bowler","year":"2001","journal-title":"Clin. Microbiol. Rev."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/8\/966\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:37:21Z","timestamp":1760164641000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/8\/966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,30]]},"references-count":21,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["pathogens10080966"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10080966","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,30]]}}}