{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:57:56Z","timestamp":1777712276182,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundo Nacional de Sa\u00fade\/Minit\u00e9rio da Sa\u00fade\/Brazil","award":["TC 334\/2013, SIAFI 677.202- FNS\/FIOCRUZ, Processo Fiocruz no 25000.204745\/2013-08"],"award-info":[{"award-number":["TC 334\/2013, SIAFI 677.202- FNS\/FIOCRUZ, Processo Fiocruz no 25000.204745\/2013-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Regional differences in tick-borne disease epidemiology may be related to biological variations between vector populations. Amblyomma aureolatum (Ixodida: Ixodidae), a neotropical tick, is known from several regions in Brazil. However, only in the metropolitan area of S\u00e3o Paulo (SP) state are there studies that establish its role as a vector of a pathogenic rickettsia (Rickettsia rickettsii). The aim of the study was to analyze the genetic diversity, population dynamics, and rickettsia infection in A. aureolatum populations from different spotted fever scenarios in Brazil. Samples were subjected to DNA extraction, amplification and sequencing of 12S rDNA, cytochrome oxidase subunit II and D-loop mitochondrial markers for tick population analyses, and gltA, htrA, ompA, and ompB genes for rickettsia researches. Of the 7\u201317 tick haplotypes identified, 5\u201313 were exclusive to each population and 2\u201312 for each epidemiological scenario, as well as three haplotypes shared by all populations. Amblyomma aureolatum populations are expanding, and do not appear to be genetically structured vis-a-vis the different epidemiological scenarios studied. Rickettsia bellii (in SP) and Rickettsia felis (in Santa Catarina) were identified as infecting A. aureolatum. No relationship between tick haplotypes and rickettsia types were observed.<\/jats:p>","DOI":"10.3390\/pathogens10091146","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T21:47:38Z","timestamp":1630964858000},"page":"1146","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Amblyomma aureolatum Genetic Diversity and Population Dynamics Are Not Related to Spotted Fever Epidemiological Scenarios in Brazil"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7812-9336","authenticated-orcid":false,"given":"Karla","family":"Bitencourth","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Sa\u00fade, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marinete","family":"Amorim","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5493-2765","authenticated-orcid":false,"given":"Stefan","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Secretaria de Vigil\u00e2ncia em Sa\u00fade, Minist\u00e9rio da Sa\u00fade, Unidade T\u00e9cnica de Vigil\u00e2ncia de Zoonoses, Bras\u00edlia 70719-040, DF, Brazil"},{"name":"Faculdade de Medicina, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38400-902, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"Gaz\u00eata","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biodiversidade e Sa\u00fade, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21045-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,6]]},"reference":[{"key":"ref_1","first-page":"27","article-title":"Ecology, biology and distribution of spotted fever tick vectors in Brazil","volume":"27","author":"Pinter","year":"2013","journal-title":"Front. 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