{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:13:26Z","timestamp":1775060006728,"version":"3.50.1"},"reference-count":21,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001054","name":"Meat and Livestock Australia","doi-asserted-by":"publisher","award":["B.AHE.0318"],"award-info":[{"award-number":["B.AHE.0318"]}],"id":[{"id":"10.13039\/501100001054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DE190100238"],"award-info":[{"award-number":["DE190100238"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37\/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12\/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies\/\u00b5L C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.<\/jats:p>","DOI":"10.3390\/pathogens10091157","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T10:12:03Z","timestamp":1631095923000},"page":"1157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner\u2019s Perspective"],"prefix":"10.3390","volume":"10","author":[{"given":"Tom","family":"Clune","sequence":"first","affiliation":[{"name":"Centre for Animal Production and Health, Murdoch University, South Street, Murdoch, Perth, WA 6150, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Anstey","sequence":"additional","affiliation":[{"name":"Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Sunshine Coast, QLD 4557, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasilli","family":"Kasimov","sequence":"additional","affiliation":[{"name":"Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Sunshine Coast, QLD 4557, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9427-1941","authenticated-orcid":false,"given":"Caroline","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Centre for Animal Production and Health, Murdoch University, South Street, Murdoch, Perth, WA 6150, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Jelocnik","sequence":"additional","affiliation":[{"name":"Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Sunshine Coast, QLD 4557, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Walker, E., Moore, C., Shearer, P., Jelocnik, M., Bommana, S., Timms, P., and Polkinghorne, A. 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