{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:11:02Z","timestamp":1790777462012,"version":"4.1.0"},"reference-count":22,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014189","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry","doi-asserted-by":"publisher","award":["320061-2"],"award-info":[{"award-number":["320061-2"]}],"id":[{"id":"10.13039\/501100014189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens. Nicotinamide adenine dinucleotide (NAD)-independent MS was first reported in 1975. Despite the atypical traits of NAD-independent MS, its independence from NAD has not been studied. In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. The assembled genomes were compared with the genomes of MS-H vaccine strain and type strain WVU1853. We found that the coding sequences of nicotinate phosphoribosyltransferase and glycerol-3-phosphate acyltransferase, and a unique coding sequence were present only in the genomes of NAD-independent isolates.<\/jats:p>","DOI":"10.3390\/pathogens10101231","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T00:30:33Z","timestamp":1632443433000},"page":"1231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea"],"prefix":"10.3390","volume":"10","author":[{"given":"Yongjun","family":"Song","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Min","family":"La","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taesoo","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyuhee","family":"Ahn","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won","family":"Hur","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjin","family":"Shin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-un","family":"Song","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunjin","family":"Park","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joong-Bok","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Yong","family":"Park","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In-Soo","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Won","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"ref_1","first-page":"845","article-title":"Mycoplasma synoviae infection","volume":"12","author":"Kleven","year":"2008","journal-title":"Dis. Poult."},{"key":"ref_2","unstructured":"Olson, N.O., Bletner, J.K., Shelton, D.C., Munro, D.A., and Anderson, G.C. (1954). Enlarged Joint Condition in Poultry Caused by an Infectious Agent, Poultry Science Association."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"36","DOI":"10.2307\/1588026","article-title":"Cultivation of the infectious-synovitis-type pleuropneumonia-like organisms","volume":"6","author":"Chalquest","year":"1962","journal-title":"Avian. Dis."},{"key":"ref_4","first-page":"2163","article-title":"A medium for the isolation of avian mycoplasmas","volume":"29","author":"Frey","year":"1968","journal-title":"Am. J. Vet. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"387","DOI":"10.2307\/1588805","article-title":"The Nicotine Adenine Dinucleotide Requirement of Mycoplasma synoviae","volume":"16","author":"Olson","year":"1972","journal-title":"Avian. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"544","DOI":"10.2307\/1589080","article-title":"Growth of Mycoplasma synoviae in a medium supplemented with nicotinamide instead of B-nicotinamide adenine dinucleotide","volume":"19","author":"DaMassa","year":"1975","journal-title":"Avian. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0034-5288(18)31878-2","article-title":"Diversity in nicotinamide-adenine dinucleotide requirement for the growth of different strains of Mycoplasma synoviae","volume":"37","author":"Yagihashi","year":"1984","journal-title":"Res. Vet. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kreizinger, Z., Sulyok, K.M., Gr\u00f3zner, D., Bek\u0151, K., D\u00e1n, \u00c1., Szab\u00f3, Z., and Gyuranecz, M. (2017). Development of mismatch amplification mutation assays for the differentiation of MS1 vaccine strain from wild-type Mycoplasma synoviae and MS-H vaccine strains. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0175969"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00055-17","DOI":"10.1128\/mSystems.00055-17","article-title":"Comparative Metabolomics of Mycoplasma bovis and Mycoplasma gallisepticum Reveals Fundamental Differences in Active Metabolic Pathways and Suggests Novel Gene Annotations","volume":"2","author":"Masukagami","year":"2017","journal-title":"mSystems"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhu, L., Shahid, M.A., Markham, J., Browning, G.F., Noormohammadi, A.H., and Marenda, M.S. (2018). Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4501-8"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00563-15","DOI":"10.1128\/genomeA.00563-15","article-title":"Complete genome sequence of Mycoplasma synoviae strain WVU 1853T","volume":"3","author":"May","year":"2015","journal-title":"Genome Announc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Alikhan, N.F., Petty, N.K., Ben Zakour, N.L., and Beatson, S.A. (2011). BLAST Ring Image Generator (BRIG): Simple prokaryote genome comparisons. BMC Genom., 12.","DOI":"10.1186\/1471-2164-12-402"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: Rapid large-scale prokaryote pan genome analysis","volume":"31","author":"Page","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3389\/fvets.2021.640067","article-title":"Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics","volume":"8","author":"Xu","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/pro.3711","article-title":"KEGG Mapper for inferring cellular functions from protein sequences","volume":"29","author":"Kanehisa","year":"2020","journal-title":"Protein Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.1073\/pnas.0811718106","article-title":"Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis","volume":"106","author":"Sorci","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1093\/bioinformatics\/btv383","article-title":"Bandage: Interactive visualization of de novo genome assemblies","volume":"31","author":"Wick","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1093\/bioinformatics\/btn322","article-title":"Database indexing for production MegaBLAST searches","volume":"24","author":"Morgulis","year":"2008","journal-title":"Bioinformatics"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/10\/1231\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:03:59Z","timestamp":1760166239000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/10\/1231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,23]]},"references-count":22,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["pathogens10101231"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10101231","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,23]]}}}