{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T12:20:52Z","timestamp":1783599652083,"version":"3.55.0"},"reference-count":66,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Feline coronavirus (FCoV) is endemic in cat populations worldwide. Persistently, subclinically infected cats play a significant role in spreading the infection. Testing fecal samples of cats may facilitate efforts to decrease the viral burden within a population. Real-time RT-PCR is highly sensitive and specific for the detection of FCoV but must be performed in a fully equipped laboratory. A simple and accurate assay is needed to identify FCoV at the point-of-need. The aim of this study was to develop a rapid FCoV detection assay based on isothermal amplification technology, i.e., reverse transcription-recombinase polymerase amplification (RT-RPA). Primers were designed to target the highly conserved 3\u2032 untranslated region of the 7b gene. Running on a constant temperature of 42 \u00b0C, reverse transcription as well as DNA amplification and detection was achieved in a maximum of 15 min. A probit analysis revealed a detection limit of 58.5 RNA copies\/reaction. For cross-detection, nucleic acids from 19 viruses were tested. Both RT-RPA and real-time RT-PCR showed cross-detection with canine coronavirus and transmissible gastroenteritis virus, but not with other pathogens. To evaluate clinical performance, RNA was extracted from 39 fecal samples from cats. All samples were tested simultaneously with real-time RT-PCR resulting in a RT-RPA sensitivity and specificity of 90.9% and 100%, respectively. RT-RPA can be considered a promising simple method for rapid detection of FCoV.<\/jats:p>","DOI":"10.3390\/pathogens10101237","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T21:40:30Z","timestamp":1632778830000},"page":"1237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay"],"prefix":"10.3390","volume":"10","author":[{"given":"Rea Maja","family":"Kobialka","sequence":"first","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Ceruti","sequence":"additional","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Bergmann","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, LMU, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Clinic of Small Animal Medicine, LMU, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Truyen","sequence":"additional","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3347-6075","authenticated-orcid":false,"given":"Ahmed","family":"Abd El Wahed","sequence":"additional","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,25]]},"reference":[{"key":"ref_1","unstructured":"King, A.M.Q., Adams, M.J., Carstens, E.B., and Lefkowitz, E.J. (2011). Coronaviridae. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses, Elsevier Academic Press. Coronaviridae."},{"key":"ref_2","first-page":"363","article-title":"Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture","volume":"42","author":"Pedersen","year":"1981","journal-title":"Am. J. Veter. Res."},{"key":"ref_3","first-page":"368","article-title":"An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis","volume":"42","author":"Pedersen","year":"1981","journal-title":"Am. J. Veter. Res."},{"key":"ref_4","first-page":"157","article-title":"Some important disorders of cats","volume":"53","author":"Holzworth","year":"1963","journal-title":"Cornell Vet."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Klein-Richers, U., Hartmann, K., Hofmann-Lehmann, R., Unterer, S., Bergmann, M., Rieger, A., Leutenegger, C., Pantchev, N., Balzer, J., and Felten, S. (2020). Prevalence of Feline Coronavirus Shedding in German Catteries and Associated Risk Factors. Viruses, 12.","DOI":"10.3390\/v12091000"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/BF01310494","article-title":"Characterization of monoclonal antibodies against feline infectious peritonitis virus type II and antigenic relationship between feline, porcine, and canine coronaviruses","volume":"117","author":"Hohdatsu","year":"1991","journal-title":"Arch. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.vetmic.2003.07.010","article-title":"Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis","volume":"99","author":"Benetka","year":"2004","journal-title":"Veter. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1292\/jvms.54.557","article-title":"The Prevalence of Types I and II Feline Coronavirus Infections in Cats","volume":"54","author":"Hohdatsu","year":"1992","journal-title":"J. Veter. Med Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1099\/vir.0.19129-0","article-title":"Persistence and transmission of natural type I feline coronavirus infection","volume":"84","author":"Addie","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0378-1135(99)00099-1","article-title":"The molecular dynamics of feline coronaviruses","volume":"69","author":"Rottier","year":"1999","journal-title":"Veter. Microbiol."},{"key":"ref_11","first-page":"7","article-title":"An Overview of Feline Enteric Coronavirus and Infectious Peritonitis Virus Infections","volume":"23","author":"Pedersen","year":"1995","journal-title":"Feline Pract."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jfms.2003.12.009","article-title":"Secong international feline coronavirus\/feline infectious peritonitis symposium Recommendations from workshops of the second international feline coronavirus\/feline infectious peritonitis symposium","volume":"6","author":"Addie","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jfms.2004.01.003","article-title":"Risk factors for feline coronavirus seropositivity in cats relinquished to a UK rescue charity","volume":"6","author":"Cave","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jfms.2003.08.008","article-title":"Common virus infections in cats, before and after being placed in shelters, with emphasis on feline enteric coronavirus","volume":"6","author":"Pedersen","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jfms.2008.09.008","article-title":"A review of feline infectious peritonitis virus infection: 1963\u20132008","volume":"11","author":"Pedersen","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1136\/vr.148.21.649","article-title":"Use of a reverse-transcriptase polymerase chain reaction for monitoring the shedding of feline coronavirus by healthy cats","volume":"148","author":"Addie","year":"2001","journal-title":"Veter. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.2460\/javma.1997.210.09.1307","article-title":"Patterns of feline coronavirus infection and fecal shedding from cats in multiple-cat environments","volume":"210","author":"Foley","year":"1997","journal-title":"J. Am. Vet. Med Assoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1051\/vetres\/2010043","article-title":"Pathogenic characteristics of persistent feline enteric coronavirus infection in cats","volume":"41","author":"Vogel","year":"2010","journal-title":"Veter. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.jfms.2008.02.006","article-title":"Pathogenesis of feline enteric coronavirus infection","volume":"10","author":"Pedersen","year":"2008","journal-title":"J. Feline Med. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.3201\/eid1807.120143","article-title":"Spike Protein Fusion Peptide and Feline Coronavirus Virulence","volume":"18","author":"Chang","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1136\/vr.130.7.133","article-title":"A study of naturally occurring feline coronavirus infections in kittens","volume":"130","author":"Addie","year":"1992","journal-title":"Veter. Rec."},{"key":"ref_22","first-page":"1193","article-title":"Effect of feline interferon-omega on the survival time and quality of life of cats with feline infectious peritonitis","volume":"21","author":"Ritz","year":"2007","journal-title":"J. Vet. Intern. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1111\/jvim.15780","article-title":"Antiviral treatment using the adenosine nucleoside analogue GS-441524 in cats with clinically diagnosed neurological feline infectious peritonitis","volume":"34","author":"Dickinson","year":"2020","journal-title":"J. Veter. Intern. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Addie, D., Covell-Ritchie, J., Jarrett, O., and Fosbery, M. (2020). Rapid Resolution of Non-Effusive Feline Infectious Peritonitis Uveitis with an Oral Adenosine Nucleoside Analogue and Feline Interferon Omega. Viruses, 12.","DOI":"10.3390\/v12111216"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1177\/1098612X15625354","article-title":"Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of feline infectious peritonitis","volume":"19","author":"Doenges","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.jfms.2007.10.010","article-title":"Evaluation of real-time RT-PCR for the quantification of FCoV shedding in the faeces of domestic cats","volume":"10","author":"Dye","year":"2008","journal-title":"J. Feline Med. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0166-0934(98)00129-3","article-title":"One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses","volume":"77","author":"Gut","year":"1999","journal-title":"J. Virol. Methods."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1128\/jcm.33.3.684-689.1995","article-title":"Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR","volume":"33","author":"Herrewegh","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_29","first-page":"1","article-title":"Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitis","volume":"48","author":"Barker","year":"2017","journal-title":"Veter. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.jviromet.2018.03.003","article-title":"Detection of feline Coronavirus in effusions of cats with and without feline infectious peritonitis using loop-mediated isothermal amplification","volume":"256","author":"Felten","year":"2018","journal-title":"J. Virol. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1039\/C8AN01621F","article-title":"Review: A comprehensive summary of a decade development of the recombinase polymerase amplification","volume":"144","author":"Li","year":"2019","journal-title":"Anal."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"El Wahed, A.A., El-Deeb, A., El-Tholoth, M., El Kader, H.A., Ahmed, A., Hassan, S., Hoffmann, B., Haas, B., Shalaby, M.A., and Hufert, F.T. (2013). A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus. PLoS ONE., 8.","DOI":"10.1371\/journal.pone.0071642"},{"key":"ref_34","unstructured":"El Wahed, A.A., Patel, P., Heidenreich, D., Hufert, F.T., and Weidmann, M. (2013). Reverse Transcription Recombinase Polymerase Amplification Assay for the Detection of Middle East Respiratory Syndrome Coronavirus. PLoS Curr., 5."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/JCM.02704-12","article-title":"Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents","volume":"51","author":"Euler","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.jcv.2012.05.006","article-title":"Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus","volume":"54","author":"Euler","year":"2012","journal-title":"J. Clin. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1128\/JCM.06504-11","article-title":"Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis","volume":"50","author":"Euler","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jcv.2015.05.004","article-title":"Diagnostics-in-a-Suitcase: Development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus","volume":"69","author":"Weidmann","year":"2015","journal-title":"J. Clin. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1126\/science.7041255","article-title":"Rapid evolution of RNA genomes","volume":"215","author":"Holland","year":"1982","journal-title":"Science."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4951","DOI":"10.1128\/jvi.66.8.4951-4956.1992","article-title":"A novel glycoprotein of feline infectious peritonitis coronavirus contains a KDEL-like endoplasmic reticulum retention signal","volume":"66","author":"Vennema","year":"1992","journal-title":"J. Virol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1177\/1098612X18809165","article-title":"Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates","volume":"21","author":"Dunbar","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jfms.2005.07.002","article-title":"Natural FCoV infection: Cats with FIP exhibit significantly higher viral loads than healthy infected cats","volume":"8","author":"Kipar","year":"2006","journal-title":"J. Feline Med. Surg."},{"key":"ref_43","unstructured":"TwistDx\u2122 (2021, August 04). TwistAmp\u00ae DNA Amplification Kits Assay Design Manual. Available online: https:\/\/www.twistdx.co.uk."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1021\/acs.analchem.0c04779","article-title":"Suitcase Lab for rapid detection of SARS-CoV-2 based on recombinase polymerase amplification assay","volume":"93","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1099\/0022-1317-73-11-2849","article-title":"Analysis of a 9.6 kb sequence from the 3\u2032 end of canine coronavirus genomic RNA","volume":"73","author":"Horsburgh","year":"1992","journal-title":"J. Gen. Virol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/0042-6822(92)90174-N","article-title":"Genomic organization and expression of the 3\u2032 end of the canine and feline enteric coronaviruses","volume":"191","author":"Vennema","year":"1992","journal-title":"Virology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1099\/0022-1317-68-4-995","article-title":"Intracellular RNAs of the Feline Infectious Peritonitis Coronavirus Strain 79-1146","volume":"68","author":"Horzinek","year":"1987","journal-title":"J. Gen. Virol."},{"key":"ref_48","first-page":"592","article-title":"Experimental inoculation of cats with canine coronavirus and subsequent challenge with feline infectious peritonitis virus","volume":"34","author":"Sorresso","year":"1984","journal-title":"Lab. Anim. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3134","DOI":"10.1128\/JVI.01394-09","article-title":"Recombination, Reservoirs, and the Modular Spike: Mechanisms of Coronavirus Cross-Species Transmission","volume":"84","author":"Graham","year":"2010","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Bonney, L.C., Watson, R.J., Afrough, B., Mullojonova, M., Dzhuraeva, V., Tishkova, F., and Hewson, R. (2017). A recombinase polymerase amplification assay for rapid detection of Crimean-Congo Haemorrhagic fever Virus infection. PLOS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0006013"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"El Wahed, A.A., Patel, P., Faye, O., Thaloengsok, S., Heidenreich, D., Matangkasombut, P., Manopwisedjaroen, K., Sakuntabhai, A., Sall, A.A., and Hufert, F.T. (2015). Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection. PLoS ONE., 10.","DOI":"10.1371\/journal.pone.0129682"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jviromet.2017.01.009","article-title":"Reverse transcriptase loop-mediated isothermal amplification for the detection of feline coronavirus","volume":"243","author":"Stranieri","year":"2017","journal-title":"J. Virol. Methods"},{"key":"ref_53","unstructured":"tamaVet GmbH (2021, August 04). tamaVet\u00ae Schnelltests f\u00fcr Katzen\u2014Coronavirus. Available online: https:\/\/www.tamavet-diagnostics.com\/produkte\/schnelltests\/katze."},{"key":"ref_54","unstructured":"Fassisi GmbH (2021, August 04). Fassisi ParCo. Available online: https:\/\/www.fassisi.de\/produkte\/kleintiere\/hunde-katzen-parco\/."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"181","DOI":"10.7883\/yoken.JJID.2019.400","article-title":"Ultra-Rapid Real-Time RT-PCR Method for Detecting Middle East Respiratory Syndrome Coronavirus Using a Mobile PCR Device, PCR1100","volume":"73","author":"Shirato","year":"2020","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/00218839.2018.1517999","article-title":"Detection of chronic bee paralysis virus using ultra-rapid PCR and nested ultra-rapid PCR","volume":"58","author":"Kim","year":"2018","journal-title":"J. Apic. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e182","DOI":"10.1093\/nar\/gni181","article-title":"Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability","volume":"33","author":"Hilscher","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"9665","DOI":"10.1021\/acs.iecr.9b01209","article-title":"Small-Size Polymerase Chain Reaction Device with Improved Heat Transfer and Combined Feedforward\/Feedback Control Strategy","volume":"58","author":"Gregorini","year":"2019","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"032009","DOI":"10.1088\/1757-899X\/563\/3\/032009","article-title":"Temperature-uniformity study on transverse flux induction heating applied to rapid PCR","volume":"Volume 242","author":"Zhou","year":"2019","journal-title":"IOP Conference Series: Earth and Environmental Science"},{"key":"ref_60","first-page":"1","article-title":"Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus","volume":"12","author":"Shalaby","year":"2016","journal-title":"BMC Veter. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"101669","DOI":"10.1016\/j.mcp.2020.101669","article-title":"Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus","volume":"54","author":"Hu","year":"2020","journal-title":"Mol. Cell. Probes."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1128\/JCM.00623-15","article-title":"Field-Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae","volume":"53","author":"Liljander","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1373\/clinchem.2015.245829","article-title":"Recombinase Polymerase Amplification for Diagnostic Applications","volume":"62","author":"Daher","year":"2016","journal-title":"Clin. Chem."},{"key":"ref_64","unstructured":"R.C. Team (2020, November 04). R: A Language and Environment for Statistical Computing. Available online: http:\/\/r.meteo.uni.wroc.pl\/web\/packages\/dplR\/vignettes\/intro-dplR.pdf."},{"key":"ref_65","unstructured":"Hadley, W. (2016). Ggplot2: Elegrant Graphics for Data Analysis, Springer."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jfms.2008.09.004","article-title":"Field strain feline coronaviruses with small deletions in ORF7b associated with both enteric infection and feline infectious peritonitis","volume":"11","author":"Lin","year":"2009","journal-title":"J. Feline Med. Surg."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/10\/1237\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:04:58Z","timestamp":1760166298000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/10\/10\/1237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,25]]},"references-count":66,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["pathogens10101237"],"URL":"https:\/\/doi.org\/10.3390\/pathogens10101237","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,25]]}}}