{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:46:03Z","timestamp":1790793963108,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T00:00:00Z","timestamp":1633651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conseil R\u00e9gional Bourgogne Franche-Comt\u00e9","award":["2019Y-09121"],"award-info":[{"award-number":["2019Y-09121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The genetic diversity of the parasite Echinococcus multilocularis, the infectious agent of alveolar echinococcosis, is generally assessed on adult worms after fox necropsy. We aimed to investigate E. multilocularis polymorphism through the microsatellite EmsB marker using a noninvasive approach. We tested batches of isolated eggs (1, 5, and 10) from 19 carnivore fecal samples collected in a rural town located in a highly endemic area in France to determine the best strategy to adopt using a minimal quantity of parasite DNA while avoiding genetic profile overlapping in the analysis. Several molecular controls were performed to formally identify the Taeniidae eggs. In total, 112 egg batches were isolated and 102 EmsB electrophoregrams were obtained in duplicate. Quality sorting was performed through the Pearson correlation coefficient (r) between each EmsB duplicate. Forty-nine batches with r &gt; 0.9 remained in the analysis, mainly 5- or 10-egg batches. Three EmsB profiles were emphasized by hierarchical clustering and matched those from human lesions and adult worms previously genotyped and collected in the same area. We show that the genetic diversity of the parasite can be assessed from isolated E. multilocularis eggs in a spatiotemporal context using a noninvasive approach.<\/jats:p>","DOI":"10.3390\/pathogens10101296","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T21:37:29Z","timestamp":1633901849000},"page":"1296","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessment of the Genetic Diversity of Echinococcus multilocularis from Copro-Isolated Eggs"],"prefix":"10.3390","volume":"10","author":[{"given":"Jenny","family":"Knapp","sequence":"first","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology, University Hospital of Besan\u00e7on, 25030 Besan\u00e7on, France"},{"name":"Laboratoire Chrono-Environnement, UMR CNRS 6249 Universit\u00e9 Bourgogne-Franche-Comt\u00e9, 16 Route de Gray, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdou Malik","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Laboratoire Chrono-Environnement, UMR CNRS 6249 Universit\u00e9 Bourgogne-Franche-Comt\u00e9, 16 Route de Gray, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Courquet","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology, University Hospital of Besan\u00e7on, 25030 Besan\u00e7on, France"},{"name":"Laboratoire Chrono-Environnement, UMR CNRS 6249 Universit\u00e9 Bourgogne-Franche-Comt\u00e9, 16 Route de Gray, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5939-3409","authenticated-orcid":false,"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology, University Hospital of Besan\u00e7on, 25030 Besan\u00e7on, France"},{"name":"Laboratoire Chrono-Environnement, UMR CNRS 6249 Universit\u00e9 Bourgogne-Franche-Comt\u00e9, 16 Route de Gray, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S87","DOI":"10.1017\/S0031182003004153","article-title":"Epidemiology of alveolar echinococcosis with particular reference to China and Europe","volume":"127","author":"Vuitton","year":"2003","journal-title":"Parasitology"},{"key":"ref_2","first-page":"213","article-title":"Ecology and life cycle patterns of echinococcus species","volume":"95","author":"Romig","year":"2017","journal-title":"Highlighting Oper. 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