{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:15:52Z","timestamp":1784085352288,"version":"3.55.0"},"reference-count":64,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T00:00:00Z","timestamp":1634256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANRS and Association pour la Recherche sur le Cancer","award":["ARC\/SUBV\/CKLQ6"],"award-info":[{"award-number":["ARC\/SUBV\/CKLQ6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Chronic hepatitis C virus (HCV) infection often leads to fibrosis and chronic hepatitis, then cirrhosis and ultimately hepatocellular carcinoma (HCC). The processes of the HVC life cycle involve intimate interactions between viral and host cell proteins and lipid metabolism. However, the molecules and mechanisms involved in this tripartite interaction remain poorly understood. Herein, we show that the infection of HCC-derived Huh7.5 cells with HCV promotes upregulation of the protein inhibitor of activated STAT1 (PIAS1). Reciprocally, PIAS1 regulated the expression of HCV core protein and HCV-induced LD accumulation and impaired HCV replication. Furthermore, PIAS1 controlled HCV-promoted septin 9 filament formation and microtubule polymerization. Subsequently, we found that PIAS1 interacted with septin 9 and controlled its assembly on filaments, which thus affected septin 9-induced lipid droplet accumulation. Taken together, these data reveal that PIAS1 regulates the accumulation of lipid droplets and offer a meaningful insight into how HCV interacts with host proteins.<\/jats:p>","DOI":"10.3390\/pathogens10101327","type":"journal-article","created":{"date-parts":[[2021,10,17]],"date-time":"2021-10-17T23:05:47Z","timestamp":1634511947000},"page":"1327","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["PIAS1 Regulates Hepatitis C Virus-Induced Lipid Droplet Accumulation by Controlling Septin 9 and Microtubule Filament Assembly"],"prefix":"10.3390","volume":"10","author":[{"given":"Abdellah","family":"Akil","sequence":"first","affiliation":[{"name":"Unit\u00e9 1193, INSERM, F-94800 Villejuif, France"},{"name":"UMR-S 1193, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"},{"name":"Department of Radiation Oncology, University of Alabama at Birmingham, Birmingham, AL 35205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4224-0750","authenticated-orcid":false,"given":"Peixuan","family":"Song","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1193, INSERM, F-94800 Villejuif, France"},{"name":"UMR-S 1193, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8407-9793","authenticated-orcid":false,"given":"Juan","family":"Peng","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1193, INSERM, F-94800 Villejuif, France"},{"name":"UMR-S 1193, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Gondeau","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1183, INSERM, Universit\u00e9 Montpellier 1, F-34293 Montpellier, France"},{"name":"Department of Hepato-Gastroenterology A, Institute of Research in Biotherapy, CHU Montpellier, Hopital Saint Eloi, F-34293 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Samuel","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1193, INSERM, F-94800 Villejuif, France"},{"name":"UMR-S 1193, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"},{"name":"Centre H\u00e9pato-Biliaire, H\u00f4pital Paul-Brousse, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9878-7811","authenticated-orcid":false,"given":"Ama","family":"Gassama-Diagne","sequence":"additional","affiliation":[{"name":"Unit\u00e9 1193, INSERM, F-94800 Villejuif, France"},{"name":"UMR-S 1193, Universit\u00e9 Paris-Saclay, F-94800 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"ref_1","first-page":"58","article-title":"Viral Hepatitis as a Risk Factor for the Development of Hepatocellular Carcinoma","volume":"6","author":"Alqahtani","year":"2020","journal-title":"Hepatoma Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wu, P.-S., Chang, T.-S., Lu, S.-N., Su, H.-J., Chang, S.-Z., Hsu, C.-W., and Chen, M.-Y. 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