{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:38:56Z","timestamp":1760236736972,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["400905\/2013-2"],"award-info":[{"award-number":["400905\/2013-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfeicoamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2072\/2013"],"award-info":[{"award-number":["2072\/2013"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006181","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado da Bahia","doi-asserted-by":"publisher","award":["1534\/2013"],"award-info":[{"award-number":["1534\/2013"]}],"id":[{"id":"10.13039\/501100006181","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The spleen is involved in visceral leishmaniasis immunopathogenesis, and presents alterations in white-pulp microenvironments that are associated with an increased susceptibility to coinfections and patient death. Plasmacytosis in splenic red pulp (RP) is one observed alteration, but the specificity of antibody-secreting cells and the distribution of them has not yet been evaluated. We biotinylated soluble L. infantum membrane antigens (bSLMA) used as probes in modified immunohistochemistry, and detected the presence of anti-L. infantum antibody-secreting cells. Were used spleens from eight dogs from the endemic area for canine visceral leishmaniasis (CanL), and three healthier controls. The spleen sections were cryopreserved, and we performed modified immunohistochemistry. The ratio of plasma cells which were reactive to bSLMA (Anti-Leish-PC) in the spleen RP and periarteriolar lymphatic sheath (PALS) were calculated. Dogs with CanL present hyperglobulinemia and more plasma cells in their RP than the controls. Furthermore, dogs with CanL presented a lower proportion of Anti-Leish-PC in their RP than in PALS. Likewise, dysproteinemia was related to RP and PALS plasmacytosis, and a more severe clinical profile.<\/jats:p>","DOI":"10.3390\/pathogens10121635","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T02:50:18Z","timestamp":1639968618000},"page":"1635","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti-Leishmania infantum Antibody-Producing Plasma Cells in the Spleen in Canine Visceral Leishmaniasis"],"prefix":"10.3390","volume":"10","author":[{"given":"Jonathan L. M.","family":"Fontes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-873X","authenticated-orcid":false,"given":"Bianca R.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginaldo","family":"Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0539-8009","authenticated-orcid":false,"given":"Juliana C. S.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline V. B.","family":"de Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5075-1254","authenticated-orcid":false,"given":"Washington L. C.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia Estrutural e Molecular, Instituto Gon\u00e7alo Moniz, Fiocruz-BA, Salvador 40296710, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2010). World Health Organization Control of the Leishmaniases, World Health Organization Technical Report Series."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2147\/CLEP.S44267","article-title":"Epidemiology of visceral leishmaniasis","volume":"6","author":"Ready","year":"2014","journal-title":"Clin. 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