{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:15:10Z","timestamp":1770963310731,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T00:00:00Z","timestamp":1642118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Tunisian Ministry of Higher Education","award":["Research laboratory annual grant"],"award-info":[{"award-number":["Research laboratory annual grant"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Leishmaniasis is a protozoal vector-borne disease that affects both humans and animals. In the Mediterranean Basin, the primary reservoir hosts of Leishmania spp. are mainly rodents and canids. Lipidomic approaches have allowed scientists to establish Leishmania spp. lipid profiles for the identification of cell stage specific biomarkers, drug mechanisms of action, and host immune response. Using an in silico approach of global network interaction between genes involved in fatty acid (FA) synthesis followed by the GC-MS approach, we were able to characterize the fatty acid profiles of L. major derived from human and rodent hosts. Our results revealed that the lipid profile of L. major showed similarities and differences with those already reported for other Leishmania species. Phospholipids are the predominant lipid class. FA composition of rodent parasites was characterized by a lower abundance of the precursor C18:2(n-6). One of the rodent clones, which also expressed the lowest lipid abundance in PL and TAG, was the least sensitive clone to the miltefosine drug and has the lowest infection efficiency. Our findings suggest that the lipid composition variation may explain the response of the parasite toward treatment and their ability to infect their host.<\/jats:p>","DOI":"10.3390\/pathogens11010092","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T03:14:03Z","timestamp":1642130043000},"page":"92","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fatty Acid Profiles of Leishmania major Derived from Human and Rodent Hosts in Endemic Cutaneous Leishmaniasis Areas of Tunisia and Algeria"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-7532","authenticated-orcid":false,"given":"Cyrine","family":"Bouabid","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie M\u00e9dicale, Biotechnologies et Biomol\u00e9cules (LR16IPT06), Institut Pasteur de Tunis, Universit\u00e9 Tunis El-Manar, 13 Place Pasteur-BP74, Tunis 1002, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiki","family":"Yamaryo-Bott\u00e9","sequence":"additional","affiliation":[{"name":"ApicoLipid Team, Institute for Advanced Biosciences, CNRS UMR5309, INSERM\u2014National Institute for Health and Medical Research, Universit\u00e9 Grenoble Alpes, INSERM U1209, 38000 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sameh","family":"Rabhi","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie M\u00e9dicale, Biotechnologies et Biomol\u00e9cules (LR16IPT06), Institut Pasteur de Tunis, Universit\u00e9 Tunis El-Manar, 13 Place Pasteur-BP74, Tunis 1002, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifa","family":"Bichiou","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie M\u00e9dicale, Biotechnologies et Biomol\u00e9cules (LR16IPT06), Institut Pasteur de Tunis, Universit\u00e9 Tunis El-Manar, 13 Place Pasteur-BP74, Tunis 1002, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-2480","authenticated-orcid":false,"given":"Chaima","family":"Hkimi","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics, BioMathematics and Biostatistics, Institut Pasteur de Tunis, 13 Place Pasteur-BP74, Tunis 1002, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wafa","family":"Bouglita","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie M\u00e9dicale, Biotechnologies et Biomol\u00e9cules (LR16IPT06), Institut Pasteur de Tunis, Universit\u00e9 Tunis El-Manar, 13 Place Pasteur-BP74, Tunis 1002, Tunisia"},{"name":"Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Tunis 2050, 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