{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:45:22Z","timestamp":1790196322735,"version":"4.1.0"},"reference-count":48,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dutch Ministry of Agriculture, Nature and Food Quality","award":["WOT-01-003-010-01"],"award-info":[{"award-number":["WOT-01-003-010-01"]}]},{"DOI":"10.13039\/501100011055","name":"Danish Ministry of Environment and Food","doi-asserted-by":"publisher","award":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"],"award-info":[{"award-number":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"]}],"id":[{"id":"10.13039\/501100011055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001734","name":"University of Copenhagen","doi-asserted-by":"publisher","award":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"],"award-info":[{"award-number":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"]}],"id":[{"id":"10.13039\/501100001734","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009527","name":"Statens Serum Institut","doi-asserted-by":"publisher","award":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"],"award-info":[{"award-number":["No grant number - work was done as part of the sovereign duties as Danish national reference laboratory"]}],"id":[{"id":"10.13039\/501100009527","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Federal Ministry of Food and Agriculture","award":["No grant number - work was done as part of the sovereign duties as German national reference laboratory"],"award-info":[{"award-number":["No grant number - work was done as part of the sovereign duties as German national reference laboratory"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>African swine fever is a viral disease of the family Suidae. Methods to detect and quantify African swine fever virus (ASFV) include qPCR and virus infectivity assays. Individual laboratories often use in-house procedures for these assays, which can hamper the comparison of results. The objective of this study was to estimate the probability of ASFV detection using these assays, and to determine the inter-test correlations between results. This was achieved by testing a panel of 80 samples at three reference laboratories. Samples were analysed using nucleic acid extraction and qPCR, as well as virus infectivity assays. For qPCR, a very high probability (ranging from 0.96 to 1.0) of detecting ASFV DNA was observed for all tested systems. For virus infectivity assays in cells, the probability of detecting infectious ASFV varied from 0.68 to 0.90 and was highest using pulmonary alveolar macrophages, followed by MARC145 cells, peripheral blood monocytes, and finally wild boar lung cells. Intraclass correlation coefficient estimates of 0.97 (0.96\u20130.98) between qPCR methods, 0.80 (0.74\u20130.85) to 0.94 (0.92\u20130.96) between virus infectivity assays, and 0.77 (0.68\u20130.83) to 0.95 (0.93\u20130.96) between qPCR methods and virus infectivity assays were obtained. These findings show that qPCR gives the highest probability for the detection of ASFV.<\/jats:p>","DOI":"10.3390\/pathogens11030325","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T01:53:54Z","timestamp":1646790834000},"page":"325","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Multi-Laboratory Comparison of Methods for Detection and Quantification of African Swine Fever Virus"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6161-3439","authenticated-orcid":false,"given":"Ann Sofie","family":"Olesen","sequence":"first","affiliation":[{"name":"DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark"},{"name":"Section of Veterinary Clinical Microbiology, Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark"},{"name":"Department of Virus & Microbiological Special Diagnostics, Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4241-1559","authenticated-orcid":false,"given":"Thomas","family":"Bruun Rasmussen","sequence":"additional","affiliation":[{"name":"DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark"},{"name":"Department of Virus & Microbiological Special Diagnostics, Statens Serum Institut, DK-2300 Copenhagen S, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2417-0787","authenticated-orcid":false,"given":"S\u00f8ren","family":"Saxmose Nielsen","sequence":"additional","affiliation":[{"name":"Section of Animal Welfare and Disease Control, Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1187-4873","authenticated-orcid":false,"given":"Graham J.","family":"Belsham","sequence":"additional","affiliation":[{"name":"DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark"},{"name":"Section of Veterinary Clinical Microbiology, Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Boklund","sequence":"additional","affiliation":[{"name":"Section of Animal Welfare and Disease Control, Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tosca","family":"Ploegaert","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Wageningen University & Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernie","family":"Moonen-Leusen","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Wageningen University & Research, 8221 RA Lelystad, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-5609","authenticated-orcid":false,"given":"Sandra","family":"Blome","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Insel Riems, 17493 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0558-0222","authenticated-orcid":false,"given":"Anette","family":"B\u00f8tner","sequence":"additional","affiliation":[{"name":"DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark"},{"name":"Section of Veterinary Clinical Microbiology, Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,7]]},"reference":[{"key":"ref_1","first-page":"613","article-title":"ICTV Report Consortium","volume":"99","author":"Alonso","year":"2018","journal-title":"ICTV Virus Taxon. 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