{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T03:44:19Z","timestamp":1784259859995,"version":"3.55.0"},"reference-count":81,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T00:00:00Z","timestamp":1650412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Minnesota State Legislature through Rapid Agricultural Response Fund (RARF)","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"Environment and Natural Resources Trust Fund through the recommendation of the Legislative-Citizen Commission on Minnesota Resources","award":["-"],"award-info":[{"award-number":["-"]}]},{"name":"University of Minnesota Office of the Vice President for Research","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Chronic wasting disease (CWD) is a transmissible prion disease of the cervidae family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However, differences in CWD transmission, stage of infection, pathogenesis, and strain can limit performance. To overcome these uncertainties, we used Bayesian statistics to assess the accuracy of RT-QuIC, an increasingly used prion amplification assay, to diagnose CWD on tonsil (TLN), parotid (PLN) and submandibular lymph nodes (SMLN), and ELISA\/IHC on RPLN of white-tailed deer (WTD) sampled from Minnesota. Dichotomous RT-QuIC and ELISA\/IHC results from wild (n = 61) and captive (n = 46) WTD were analyzed with two-dependent-test, one-population models. RT-QuIC performed on TLN and SMLN of the wild WTD population had similar sensitivity (median range (MR): 92.2\u201395.1) to ELISA\/IHC on RPLN (MR: 91.1\u201392.3). Slightly lower (4\u20137%) sensitivity estimates were obtained from farmed animal and PLN models. RT-QuIC specificity estimates were high (MR: 94.5\u201398.5%) and similar to ELISA\/IHC estimates (MR: 95.7\u201397.6%) in all models. This study offers new insights on RT-QuIC and ELISA\/IHC performance at the population level and under field conditions, an important step in CWD diagnosis and management.<\/jats:p>","DOI":"10.3390\/pathogens11050489","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T03:46:11Z","timestamp":1650512771000},"page":"489","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Assessment of Real-Time Quaking-Induced Conversion (RT-QuIC) Assay, Immunohistochemistry and ELISA for Detection of Chronic Wasting Disease under Field Conditions in White-Tailed Deer: A Bayesian Approach"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1394-9592","authenticated-orcid":false,"given":"Catalina","family":"Picasso-Risso","sequence":"first","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Facultad de Veterinaria, Universidad de la Republica, Montevideo 11200, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9664-6890","authenticated-orcid":false,"given":"Marc D.","family":"Schwabenlander","sequence":"additional","affiliation":[{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7517-0480","authenticated-orcid":false,"given":"Gage","family":"Rowden","sequence":"additional","affiliation":[{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Carstensen","sequence":"additional","affiliation":[{"name":"Minnesota Department of Natural Resources, 5463 West Broadway, Forest Lake, MN 55025, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C.","family":"Bartz","sequence":"additional","affiliation":[{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Department of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, NE 68178, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1740-4442","authenticated-orcid":false,"given":"Tiffany M.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"},{"name":"Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1146\/annurev-animal-022114-111001","article-title":"Chronic Wasting Disease of Cervids: Current Knowledge and Future Perspectives","volume":"3","author":"Haley","year":"2015","journal-title":"Annu. 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