{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T23:56:18Z","timestamp":1783727778314,"version":"3.55.0"},"reference-count":24,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,24]],"date-time":"2022-04-24T00:00:00Z","timestamp":1650758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The exacerbation of antimicrobial resistance (AMR) is a major public health threat worldwide. In sub-Saharan Africa, there is a scarcity of data regarding multidrug-resistant (resistance to at least one antibiotic of three or more families of antibiotics) as well as extended spectrum \u03b2-lactamase-producing Enterobacterales (ESBL-PE), isolated among clinical and asymptomatically healthy patients, especially in women living with HIV (WLHIV) despite their immunocompromised status. The overarching aim of this study was set to determine the prevalence and characterize genotypically multi-drug resistant Enterobacterales (MDR-E) and ESBL- PE isolated from vaginal swabs of WLHIV attending the Yaound\u00e9 Central Hospital, Yaound\u00e9, Cameroon. A cross-sectional study was conducted among WLHIV during a four-month periods from 1 February to 31 May 2021. A total of 175 WLHIV, of childbearing age and under antiretroviral treatment were contacted. One hundred and twenty participants (120) were recruited and vaginal swabs were collected from them. After culture on Eosine-Methylen Blue (EMB) agar, the identification of Enterobacterales was performed using API 20E kit. A double-screening of ESBL-PE was performed using a combined disc diffusion method and ROSCO Diagnostica kits. An antibiotic susceptibility test was carried out by disc diffusion as per the Kirby-Bauer method and the \u03b2-lactamase resistance genes, blaCTX-M, blaCTX-M-group1-2-9, blaTEM were molecularly characterized using a conventional Polymerase Chain Reaction (PCR). Overall, 30.83% (37\/120) of the included WLHIV were colonized with Enterobacterales and the prevalence of vaginal carriage of MDR Enterobacterales among them was 62.16% (23\/37). Among MDR-E isolates, the most prevalent species were E. coli (56.0%; 14\/25) and K. pneumoniae (20.0%; 5\/25). High rates of resistance to trimethoprim-sulfamethoxazole (96.0%; 24\/25), amoxicillin-clavulanic acid (88.0%; 22\/25) and gentamicin (72%; 18\/25) were observed. The resistance mechanisms detected among these isolates were ESBL (48.0%; 12\/25), ESBL+ porin loss (8.0%; 2\/25), ESBL+AmpC (24%; 6\/25), with blaCTX-M, blaCTX-M-group-1,2,9 being identified at 48.0% (12\/25) for each of them and blaTEM at 72.0% (18\/25). Our findings confirm the high-prevalence of MDR as well as ESBL-PE isolated in WLHIV, and suggest that a real time monitoring system of antimicrobial resistant bacteria coupled with the reinforcement of infection prevention control (IPC) strategies are needed to sustainably contain these life-threatening pathogens especially in the most vulnerable populations.<\/jats:p>","DOI":"10.3390\/pathogens11050504","type":"journal-article","created":{"date-parts":[[2022,4,24]],"date-time":"2022-04-24T00:45:21Z","timestamp":1650761121000},"page":"504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Multidrug-Resistant and Extended-Spectrum \u03b2-Lactamase (ESBL) - Producing Enterobacterales Isolated from Carriage Samples among HIV Infected Women in Yaound\u00e9, Cameroon"],"prefix":"10.3390","volume":"11","author":[{"given":"Ravalona Jessica","family":"Zemtsa","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang P.O. Box 96, Cameroon"},{"name":"Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 67, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Noubom","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang P.O. Box 96, Cameroon"},{"name":"Dschang District Hospital, Foto, Dschang P.O. Box 43, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3874-8175","authenticated-orcid":false,"given":"Luria Leslie","family":"Founou","sequence":"additional","affiliation":[{"name":"Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 67, Cameroon"},{"name":"Bioinformatics & Applied Machine Learning Research Unit, EDEN Biosciences Research Institute (EBRI), EDEN Foundation, Yaound\u00e9 P.O. Box 8242, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice Davy","family":"Dimani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Higher Institute of Medical Technology, Nkolondom, Yaound\u00e9 P.O. Box 188, Cameroon"},{"name":"Antimicrobial Resistance and Infectious Diseases, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 8242, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice Landry","family":"Koudoum","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang P.O. Box 96, Cameroon"},{"name":"Antimicrobial Resistance and Infectious Diseases, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 8242, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5386-8802","authenticated-orcid":false,"given":"Aurelia Djeumako","family":"Mbossi","sequence":"additional","affiliation":[{"name":"Reproductive, Maternal, Newborn and Child Health (ReMARCH) Research Unit, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 67, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Kouanfack","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang P.O. Box 96, Cameroon"},{"name":"Central Hospital of Yaound\u00e9, Yaound\u00e9 P.O. Box 47, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9866-2103","authenticated-orcid":false,"given":"Raspail Carrel","family":"Founou","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang P.O. Box 96, Cameroon"},{"name":"Antimicrobial Resistance and Infectious Diseases, Research Institute of Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM-RI), Yaound\u00e9 P.O. Box 8242, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/CMR.00048-19","article-title":"Antimicrobial Resistance Surveillance in Low- and Middle-Income Countries: Progress and Challenges in Eight South Asian and Southeast Asian Countries","volume":"33","author":"Gandra","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","first-page":"874","article-title":"313-Infections Due to Other Members of the Enterobacteriaceae, Including Management of Multidrug-Resistant Strains","volume":"2","author":"Goldman","year":"2012","journal-title":"Goldman\u2019s Cecil Medicine"},{"key":"ref_3","unstructured":"WHO (2017). Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections, Including Tuberculosis, World Health Organization."},{"key":"ref_4","unstructured":"Baux-Pomar\u00e8s, E. (2015). Traitement Des Infections \u00c0 Ent\u00e9robact\u00e9ries S\u00e9cr\u00e9trices De BLSE: Alternatives Aux Carbap\u00e9n\u00e8mes. [Ph.D. Thesis, Universit\u00e9 de Lorraine]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1099\/jmm.0.000474","article-title":"Carriage of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Hiv-Infected Children in Zimbabwe","volume":"66","author":"Wilmore","year":"2017","journal-title":"J. Med. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1089\/mdr.2020.0066","article-title":"High Prevalence of Fecal Carriage of Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae among Newly Hiv-Diagnosed Adults in a Community Setting in Tanzania","volume":"26","author":"Manyahi","year":"2020","journal-title":"Microb. Drug Resist."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e0264818","DOI":"10.1155\/2020\/5154217","article-title":"Carriage of Extended-Spectrum-Beta-Lactamase- and Ampc-Beta-Lactamase-Producing Enterobacteriaceae (Esbl-Pe) in Healthy Community and Outpatient Department (Opd) Patients in Nepal","volume":"2020","author":"Mandal","year":"2020","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_8","unstructured":"ONUSIDA (2021). Estimations \u00c9pid\u00e9miologiques Pr\u00e9liminaires De L\u2019onusida 2021. Stat. Mond. Sur Vih, 2021, 1\u20137."},{"key":"ref_9","unstructured":"Institut National De La Statistique (Ins) Et Icf (2020). International. Enqu\u00eate D\u00e9mographique et de Sant\u00e9 et \u00e0 Indicateurs Multiples du Cameroun 2018, INS et ICF International."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s12981-021-00347-x","article-title":"Fecal Carriage of Extended Spectrum Beta-Lactamase Producing Enterobacteriaceae among Hiv Infected Children at the University of Gondar Comprehensive Specialized Hospital Gondar, Ethiopia","volume":"18","author":"Bayleyegn","year":"2021","journal-title":"AIDS Res. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13756-019-0488-4","article-title":"Extended-Spectrum Beta-Lactamase Production and Multi-Drug Resistance among Enterobacteriaceae Isolated in Addis Ababa, Ethiopia","volume":"8","author":"Teklu","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.4314\/ijbcs.v11i3.19","article-title":"Pr\u00e9valence Des Souches D\u2019ent\u00e9robact\u00e9ries Productrices De B\u00eata-Lactamases \u00c0 Spectre \u00c9largi Isol\u00e9es Au Togo Et De Leur Sensibilit\u00e9 Aux Antibiotiques","volume":"11","author":"Toudji","year":"2017","journal-title":"Int. J. Biol. Chem. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mabeku Kouitcheu, L.B., Efon Ekangouo, A., and Kouam Mewa, J.E. (2020). Trends in Carriage of Extended Spectrum \u0392-Lactamase and Carbapenemase Producing Enterobacteria among Hiv Infected Patients and Their Antimicrobial Susceptibility Patterns in Western-Cameroon. Res. Sq., 1\u201320.","DOI":"10.21203\/rs.3.rs-129678\/v1"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2020.00314","article-title":"Dissemination of Carbapenemases (Kpc, Ndm, Oxa-48, Imp, and Vim) among Carbapenem-Resistant Enterobacteriaceae Isolated from Adult and Children Patients in China","volume":"10","author":"Han","year":"2020","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_15","first-page":"61","article-title":"Ampc Beta-Lactamases","volume":"22","author":"Jacoby","year":"2009","journal-title":"Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e0212042","DOI":"10.1371\/journal.pone.0212042","article-title":"Low Rate of Gut Colonization by Extended-Spectrum \u0392-Lactamase Producing Enterobacteriaceae in Hiv Infected Persons as Compared to Healthy Individuals in Nepal","volume":"14","author":"Hosuru","year":"2019","journal-title":"PLoS ONE"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/s13756-020-00856-w","article-title":"Prevalence and Molecular Characteristics of Esbl and Ampc Beta -Lactamase Producing Enterobacteriaceae Strains Isolated from Utis in Egypt","volume":"9","author":"Mohamed","year":"2020","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Lonchel, C.M., Meex, C., Gangou\u00e9-Pi\u00e9boji, J., Boreux, R., Assoumou, M.C., Melin, P., and De Mol, P. (2012). Proportion of Extended-Spectrum \u00df-Lactamase-Producing Enterobacteriaceae in Community Setting in Ngaoundere, Cameroon. BMC Infect. Dis., 12.","DOI":"10.1186\/1471-2334-12-53"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/1469-0691.12239","article-title":"Prevalence and Spread of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Ngaoundere, Cameroon","volume":"19","author":"Magoue","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_20","unstructured":"Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie (2021). CASFM\/EUCAST 2021, Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie Ed."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5347","DOI":"10.21746\/ijbio.2017.04.004","article-title":"Quality Improvement of the DNA Extracted by Boiling Method in Gram Negative Bacteria","volume":"6","author":"Ahmed","year":"2017","journal-title":"Int. J. Bioassays"},{"key":"ref_22","first-page":"710","article-title":"Development and Evaluation of a Novel Universal Beta-Lactamase Gene Sutyping Assy for Blatem, Blactx-M, Blactx-M1, 2, 9, Blashv Using Clinical and Livestock-Associated Esherichia coli","volume":"70","author":"Dahms","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1128\/AAC.49.4.1319-1322.2005","article-title":"Bla(Ctx-M) Genes in Clinical Salmonella Isolates Recovered from Humans in England and Wales from 1992 to 2003","volume":"49","author":"Batchelor","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1469-0691.2006.01645.x","article-title":"Development and Clinical Validation of a Molecular Diagnostic Assay to Detect Ctx-M-Type Beta-Lactamases in Enterobacteriaceae","volume":"13","author":"Pitout","year":"2007","journal-title":"Clin. Microbiol. Infect."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/5\/504\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:59:37Z","timestamp":1760137177000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/5\/504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,24]]},"references-count":24,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["pathogens11050504"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11050504","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,24]]}}}