{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T05:26:30Z","timestamp":1788413190375,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T00:00:00Z","timestamp":1651276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>During recent decades, a tick-borne rickettsial syndrome, characterized by eschar and painful lymphadenopathy after Dermacentor marginatus-bite, has been described as an emerging rickettsiosis in Europe. Our group named it DEBONEL (Dermacentor-borne-necrosis-erythema-lymphadenopathy), regarding the vector and the main infection signs. Other groups called it TIBOLA (tick-borne-lymphadenophathy) and, later, SENLAT (scalp-eschar-and-neck-lymphadenopathy-after-tick-bite), expanding, in the latter, the etiological spectrum to other pathogens. Objective: To investigate the etiology of DEBONEL agents in our area, and to compare their epidemiological\/clinical\/microbiological characteristics. During 2001\u20132020, 216 patients clinically diagnosed of DEBONEL (the largest series from one center) in La Rioja (northern Spain) were examined. Rickettsia spp. were amplified in 14\/104 (13.46%) blood samples, 69\/142 (48.59%) eschar swabs, 7\/7 (100%) biopsies, and 71\/71 (100%) D. marginatus from patients. For samples in which Rickettsia was undetected, no other microorganisms were found. \u2018Candidatus Rickettsia rioja\u2019, Rickettsia slovaca, Rickettsia raoultii, and Rickettsia DmS1 genotype were detected in 91, 66, 4, and 3 patients, respectively. DEBONEL should be considered in patients with clinical manifestations herein described in areas associated to Dermacentor. The most frequently involved agent in our environment is \u2018Ca. R. rioja\u2019. The finding of Rickettsia sp. DmS1 in ticks attached to DEBONEL patients suggests the implication of other rickettsia genotypes.<\/jats:p>","DOI":"10.3390\/pathogens11050528","type":"journal-article","created":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T07:08:58Z","timestamp":1651475338000},"page":"528","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Epidemiological, Clinical, and Microbiological Characteristics in a Large Series of Patients Affected by Dermacentor-Borne-Necrosis-Erythema-Lymphadenopathy from a Unique Centre from Spain"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4996-288X","authenticated-orcid":false,"given":"Sonia","family":"Santib\u00e1\u00f1ez","sequence":"first","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2055-1825","authenticated-orcid":false,"given":"Ar\u00e1nzazu","family":"Portillo","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valvanera","family":"Ibarra","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8092-1982","authenticated-orcid":false,"given":"Paula","family":"Santib\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"Metola","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepci\u00f3n","family":"Garc\u00eda-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5461-5874","authenticated-orcid":false,"given":"Ana M.","family":"Palomar","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cervera-Acedo","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Alba","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4268-0150","authenticated-orcid":false,"given":"Jos\u00e9 R.","family":"Blanco","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious Diseases Department, San Pedro University Hospital-Center for Biomedical Research from La Rioja (CIBIR), 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Oteo","sequence":"additional","affiliation":[{"name":"Center of Rickettsiosis and Arthropod-Borne Diseases (CRETAV), Infectious 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Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0213-005X(02)72740-7","article-title":"DEBONEL (Dermacentor-borne-necrosis-erythema-lymphadenopathy). \u00bfUna nueva enfermedad transmitida por garrapatas?","volume":"20","author":"Oteo","year":"2002","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.3201\/eid0901.020192","article-title":"First isolation of Rickettsia slovaca from a patient, France","volume":"9","author":"Cazorla","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1198-743X.2004.00782.x","article-title":"Dermacentor-borne necrosis erythema and lymphadenopathy: Clinical and epidemiological features of a new tick-borne disease","volume":"10","author":"Oteo","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1196\/annals.1374.040","article-title":"Rickettsia slovaca Infection: DEBONEL\/TIBOLA","volume":"1078","author":"Ibarra","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/0036554021000027016","article-title":"A case of tick-transmitted lymphadenopathy in Bulgaria associated with Rickettsia slovaca","volume":"35","author":"Komitova","year":"2003","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"817","DOI":"10.3201\/eid1405.070976","article-title":"Rickettsia slovaca in Dermacentor marginatus and tick-borne lymphadenopathy, Tuscany, Italy","volume":"14","author":"Selmi","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1097\/INF.0b013e31816b1947","article-title":"Tick-borne lymphadenopathy: A new infectious disease in children","volume":"27","author":"Porta","year":"2008","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.3201\/eid1507.081449","article-title":"Rickettsia slovaca and R. raoultii in tick-borne Rickettsioses","volume":"15","author":"Parola","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rieg, S., Schmoldt, S., Theilacker, C., de With, K., W\u00f6lfel, S., Kern, W.V., and Dobler, G. (2011). Tick-borne lymphadenopathy (TIBOLA) acquired in Southwestern Germany. BMC Infect. Dis., 11.","DOI":"10.1186\/1471-2334-11-167"},{"key":"ref_13","first-page":"583","article-title":"Case of TIBOLA\/DEBONEL (tick-borne lymphadenopathy\/Dermacentor spp.-borne necrosis-erythema-lymphadenopathy) in Poland","volume":"65","author":"Chmielewski","year":"2011","journal-title":"Przegl. Epidemiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.arcped.2011.02.018","article-title":"Dermohypodermitis on the face revealing TIBOLA","volume":"18","author":"Gaston","year":"2011","journal-title":"Arch. Pediatr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.ttbdis.2012.10.035","article-title":"Tick-borne rickettsioses in Europe","volume":"3","author":"Oteo","year":"2012","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_16","first-page":"1627","article-title":"Rickettsia slovaca infection in humans, Portugal","volume":"19","author":"Pereira","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.annder.2013.11.001","article-title":"TIBOLA: An emerging clinically polymorphous rickettsiosis","volume":"141","author":"Rigal","year":"2014","journal-title":"Ann. Dermatol. Venereol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.tmaid.2015.01.002","article-title":"Detection of Dermacentor marginatus and a possible Rickettsia slovaca case in the United Kingdom-the risk of the visiting traveller","volume":"13","author":"Pietzsch","year":"2015","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.3201\/eid2112.150672","article-title":"Tickborne Lymphadenopathy Complicated by Acute Myopericarditis, Spain","volume":"21","author":"Silva","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Barlozzari, G., Romiti, F., Zini, M., Magliano, A., De Liberato, C., Corrias, F., Capponi, G., Galli, L., Scarpulla, M., and Montagnani, C. (2021). Scalp eschar and neck lymphadenopathy by Rickettsia slovaca after Dermacentor marginatus tick bite case report: Multidisciplinary approach to a tick-borne disease. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-05807-3"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S0140-6736(05)61814-4","article-title":"A new tick-transmitted disease due to Rickettsia slovaca","volume":"350","author":"Raoult","year":"1997","journal-title":"Lancet"},{"key":"ref_22","first-page":"49","article-title":"Eritema, Necrosis y Linfedenopat\u00eda. Una nueva enfermedad (DEBONEL) transmitida por Dermacentor marginatus Sulzer, 1776","volume":"Volume 12","author":"Oteo","year":"2000","journal-title":"Zub\u00eda Monogr\u00e1fico"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1196\/annals.1355.056","article-title":"DEBONEL\/TIBOLA: Is Rickettsia slovaca the only etiological agent?","volume":"1063","author":"Ibarra","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1469-0691.2008.02250.x","article-title":"Genetic characterisation of ompA, ompB and gltA genes from Candidatus Rickettsia rioja","volume":"15","author":"Portillo","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1099\/ijs.0.64952-0","article-title":"Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia","volume":"58","author":"Mediannikov","year":"2008","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"811","DOI":"10.3201\/eid0506.990612","article-title":"New Rickettsiae in ticks collected in territories of the former soviet union","volume":"5","author":"Rydkina","year":"1999","journal-title":"Emerg. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1007\/s10096-001-0638-4","article-title":"Detection and identification of spotted fever group rickettsiae in Dermacentor ticks from Russia and central Kazakhstan","volume":"20","author":"Shpynov","year":"2001","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1086\/650172","article-title":"Scalp eschar and neck lymphadenopathy caused by Bartonella henselae after Tick Bite","volume":"50","author":"Angelakis","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/1752-1947-5-108","article-title":"Eschar and neck lymphadenopathy caused by Francisella tularensis after a tick bite: A case report","volume":"5","author":"Edouard","year":"2011","journal-title":"J. Med. Case Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"836","DOI":"10.3201\/eid1905.121169","article-title":"Scalp eschar and neck lymphadenopathy caused by Rickettsia massiliae","volume":"19","author":"Cascio","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.annder.2013.07.014","article-title":"Update on SENLAT syndrome: Scalp eschar and neck lymph adenopathy after a tick bite","volume":"140","author":"Foissac","year":"2013","journal-title":"Ann. Dermatol. Venereol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1007\/s10096-014-2090-2","article-title":"Scalp eschar and neck lymphadenopathy after tick bite: An emerging syndrome with multiple causes","volume":"33","author":"Dubourg","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.ttbdis.2016.03.008","article-title":"Rickettsia massiliae infection and SENLAT syndrome in Romania","volume":"7","author":"Zaharia","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Seo, J.W., Kim, C.M., Yun, N.R., Kim, D.M., Kim, S.S., Choi, S., and Chu, H. (2020). Scalp eschar and neck lymphadenopathy after tick bite (SENLAT) caused by Bartonella henselae in Korea: A case report. BMC Infect. Dis., 20.","DOI":"10.1186\/s12879-020-4940-0"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1196\/annals.1355.040","article-title":"Effect of antibiotic treatment in patients with DEBONEL\/TIBOLA","volume":"1063","author":"Ibarra","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ijid.2014.05.025","article-title":"Syndromic classification of rickettsioses: An approach for clinical practice","volume":"28","author":"Hidalgo","year":"2014","journal-title":"Int. J. Infect. Dis."},{"key":"ref_37","unstructured":"Oteo, J.A., Mart\u00ednez de Artola, V., and Casas, J.M. (1994, January 26\u201330). Tick-borne diseases in Spain. Proceedings of the 6th International Congress for Infectious Diseases, Prague, Czech Republic."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/S0014-2565(00)70564-9","article-title":"Eritema migratorio (borreliosis de Lyme). Caracter\u00edsticas clinicoepidemiol\u00f3gicas de 50 pacientes [Migratory erythema (Lyme borreliosis). Clinicoepidemiologic features of 50 patients]","volume":"200","year":"2000","journal-title":"Rev. Clin. Esp."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Estrada-Pe\u00f1a, A., Mihalca, A.D., and Petney, T.N. (2017). Dermacentor marginatus (Sulzer, 1776). Ticks of Europe and North Africa. A Guide to Species Identification, Springer International Publishing.","DOI":"10.1007\/978-3-319-63760-0"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.ttbdis.2014.04.016","article-title":"Tick-borne lymphadenopathy, an emerging disease","volume":"5","author":"Sarmento","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1749-6632.2003.tb07361.x","article-title":"Prevalence of Rickettsia spp. in Dermacentor marginatus ticks removed from game pigs (Sus scrofa) in southern France","volume":"990","author":"Sanogo","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1196\/annals.1374.063","article-title":"Spotted fever group rickettsiae in ticks feeding on humans in northwestern Spain: Is Rickettsia conorii vanishing?","volume":"1078","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1111\/j.1469-0691.2004.01019.x","article-title":"ESCMID Study Group on Coxiella, Anaplasma, Rickettsia and Bartonella; European Network for Surveillance of Tick-Borne Diseases. Guidelines for the diagnosis of tick-borne bacterial diseases in Europe","volume":"10","author":"Brouqui","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1196\/annals.1374.113","article-title":"Evaluation of IgG antibody response against Rickettsia conorii and Rickettsia slovaca in patients with DEBONEL\/TIBOLA","volume":"1078","author":"Santibanez","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.eimc.2012.08.001","article-title":"Usefulness of rickettsial PCR assays for the molecular diagnosis of human rickettsioses","volume":"31","author":"Portillo","year":"2013","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1128\/jb.173.5.1576-1589.1991","article-title":"Genotypic iden.ntification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes","volume":"173","author":"Regnery","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3201\/eid1102.040603","article-title":"Spotted fever group and typhus group rickettsioses in humans, South Korea","volume":"11","author":"Choi","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1128\/jcm.34.9.2058-2065.1996","article-title":"Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA","volume":"34","author":"Roux","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/JCM.39.2.430-437.2001","article-title":"Use of rpoB gene analysis for detection and identification of Bartonella species","volume":"39","author":"Renesto","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1080\/003655401750174101","article-title":"Rapid laboratory diagnosis of ulceroglandular tularemia with polymerase chain reaction","volume":"33","author":"Karhukorpi","year":"2001","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1128\/AEM.71.5.2616-2625.2005","article-title":"Molecular Identification and Analysis of Borrelia burgdorferi Sensu Lato in Lizards in the Southeastern United States","volume":"71","author":"Clark","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"730","DOI":"10.4269\/ajtmh.1992.47.730","article-title":"Detection of Borrelia burgdorferi in ticks by species-specific amplification of the flagellin gene","volume":"47","author":"Johnson","year":"1992","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1111\/j.1439-0450.1994.tb00267.x","article-title":"Detection of Coxiella burnetii in cow\u2019s milk using the polymerase chain reaction (PCR)","volume":"41","author":"Willems","year":"1994","journal-title":"J. Vet. Med."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/5\/528\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:04:55Z","timestamp":1760137495000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/5\/528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,30]]},"references-count":53,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["pathogens11050528"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11050528","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,30]]}}}