{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:13:28Z","timestamp":1760228008993,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T00:00:00Z","timestamp":1651881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871681","Z202006"],"award-info":[{"award-number":["81871681","Z202006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research Project of Wuxi Municipal Health Commission","award":["81871681","Z202006"],"award-info":[{"award-number":["81871681","Z202006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Plasmodium falciparum, mainly distributed in tropical and subtropical regions of the world, has received widespread attention owing to its severity. As a novel protein, P. falciparum surface-related antigen (PfSRA) has the structural and functional characteristics to be considered as a malaria vaccine candidate; however, limited information is available on its immunogenicity. Here, we expressed three fragments of recombinant PfSRA in an Escherichia coli system and further analyzed its immunogenicity. The results showed that rPfSRA-immunized mice produced specific antibodies with high endpoint titers (1:10,000 to 1:5,120,000) and affinity antibodies (i.e., rPfSRA-F1a (97.70%), rPfSRA-F2a (69.62%), and rPfSRA-F3a (91.87%)). In addition, the sera of immunized mice recognized both the native PfSRA and recombinant PfSRA, the rPfSRA antibodies inhibited the invasion of P. falciparum into the erythrocytes, and they were dose-dependent in vitro. This study confirmed PfSRA could be immunogenic, especially the F1a at the conserved region N-terminal and provided further support for it as a vaccine candidate against P.falciparum.<\/jats:p>","DOI":"10.3390\/pathogens11050550","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T21:31:20Z","timestamp":1652045480000},"page":"550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunogenicity Analysis of the Recombinant Plasmodium falciparum Surface-Related Antigen in Mice"],"prefix":"10.3390","volume":"11","author":[{"given":"Jia-Li","family":"Yu","sequence":"first","affiliation":[{"name":"Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Wuxi 9th Affiliated Hospital of Soochow University (Wuxi 9th People\u2019s Hospital), Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Fan","family":"Sun","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Ju","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuxi Red Cross Blood Center, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Jiang","sequence":"additional","affiliation":[{"name":"Wuxi Red Cross Blood Center, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei 230000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu-Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Wuxi 9th Affiliated Hospital of Soochow University (Wuxi 9th People\u2019s Hospital), Wuxi 214000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,7]]},"reference":[{"key":"ref_1","unstructured":"WHO (2022, May 01). 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