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Here, we investigated Rickettsia, Anaplasmataceae, and Apicomplexan pathogens in 59 and 96 adult ticks collected from dogs and cattle, respectively, in Shangombo, a town at the Zambia\u2013Angola border. We detected Richkettsia africae and Rickettsia aeschilimannii in 15.6% of Amblyomma variegatum and 41.7% of Hyalomma truncatum ticks, respectively. Ehrlichia minasensis was detected in 18.8% of H. truncatum, and Candidatus Midichloria mitochondrii was determined in Hyalomma marginatum. We also detected Babesia caballi and Theileria velifera in A. variegatum ticks with a 4.4% and 6.7% prevalence, respectively. In addition, Hepatozoon canis was detected in 6.5% of Rhipicephalus lunulatus and 4.3% of Rhipicephalus sanguineus. Coinfection of R. aeshilimannii and E. minasensis were observed in 4.2% of H. truncatum. This is the first report of Ca. M. mitochondrii and E. minasensis, and the second report of B. caballi, in the country. Rickettsia africae and R. aeschlimannii are pathogenic to humans, and E. minasensis, B. caballi, T. velifera, and H. canis are pathogenic to animals. Therefore, individuals, clinicians, veterinarians, and pet owners should be aware of the distribution of these pathogens in the area.<\/jats:p>","DOI":"10.3390\/pathogens11050566","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T21:52:11Z","timestamp":1652219531000},"page":"566","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection of Tick-Borne Bacterial and Protozoan Pathogens in Ticks from the Zambia\u2013Angola Border"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8621-5472","authenticated-orcid":false,"given":"Yongjin","family":"Qiu","sequence":"first","affiliation":[{"name":"Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Simuunza","sequence":"additional","affiliation":[{"name":"Department of Disease Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9478-0439","authenticated-orcid":false,"given":"Masahiro","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Ndebe","sequence":"additional","affiliation":[{"name":"Department of Disease Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngonda","family":"Saasa","sequence":"additional","affiliation":[{"name":"Department of Disease Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penjani","family":"Kapila","sequence":"additional","affiliation":[{"name":"Department of Disease Control, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayato","family":"Furumoto","sequence":"additional","affiliation":[{"name":"Japan International Cooperation Agency in Zambia, P.O. Box 30027, Lusaka 10101, Zambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0338-2484","authenticated-orcid":false,"given":"Alice C. C.","family":"Lau","sequence":"additional","affiliation":[{"name":"Laboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, N 18 W 9, Kita-ku, Sapporo 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3105-7603","authenticated-orcid":false,"given":"Ryo","family":"Nakao","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, N 18 W 9, Kita-ku, Sapporo 060-0818, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2464-6642","authenticated-orcid":false,"given":"Ayato","family":"Takada","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"One Health Research Center, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2569-2755","authenticated-orcid":false,"given":"Hirofumi","family":"Sawa","sequence":"additional","affiliation":[{"name":"Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"One Health Research Center, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"},{"name":"Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, N 20 W 10, Kita-ku, Sapporo 001-0020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,10]]},"reference":[{"key":"ref_1","first-page":"6938","article-title":"Overview: Ticks as vectors of pathogens that cause disease in humans and animals","volume":"13","author":"Venzal","year":"2008","journal-title":"Front. 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