{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T23:09:05Z","timestamp":1768777745063,"version":"3.49.0"},"reference-count":73,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,30]],"date-time":"2022-05-30T00:00:00Z","timestamp":1653868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004686","name":"Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University","doi-asserted-by":"publisher","award":["GRANT239"],"award-info":[{"award-number":["GRANT239"]}],"id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Uropathogenic Escherichia coli is the most common cause of urinary tract infections, resulting in about 150 million reported annual cases. With multidrug resistance on the rise and the need for global and region surveillance, this investigation looks at the UPEC isolates collected for a 3-year period, with a view of ascertaining their antimicrobial susceptibility patterns and associated virulence determinants. The identification of bacteria isolates, antimicrobial susceptibility, and extended-spectrum beta-lactamases (ESBLs) production was determined with a Vitek 2 Compact Automated System (BioMerieux, Marcy L\u2019Etoile, France). ESBLs were confirmed by the combined disc test (CDT) and basic biochemical test. The isolates were distributed into A (11%), B1 (6%), B2 (62.4%), and D (20.6%). Resistance to the penicillin group was high, between 88% and 100%. Additionally, resistance was high to cephalosporins (100%) in 2017 and 2018. The isolates were all sensitive to tigecycline, while resistance against imipenem and meropenem was low, at 4\u201312% in 2017 and 2018 and 0% in 2019. The results also showed that ESBL isolates were seen in 2017 and 2018. They were confirmed positive to CTX\/CLA (88.5%) and CAZ\/CLA (85%). By 2019, the number of resistant isolates reduced, showing only 4% ESBL isolates. Two virulence genes, fimH (46%) and papE\/F (15%), were detected among the isolates by PCR. In conclusion, this study found that phylogroups B2 and D carried the most virulence genes as well as MDR and ESBL characteristics, suggesting the UPEC strains to be extraintestinal pathogens responsible for UTIs.<\/jats:p>","DOI":"10.3390\/pathogens11060631","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:25:12Z","timestamp":1653956712000},"page":"631","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A Three-Year Look at the Phylogenetic Profile, Antimicrobial Resistance, and Associated Virulence Genes of Uropathogenic Escherichia coli"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9201-6215","authenticated-orcid":false,"given":"Lorina I.","family":"Badger-Emeka","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naheed","family":"Kausar","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edric","family":"Estrella","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenda Belgira","family":"Angeles","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1086\/318850","article-title":"Urinary tract infections: Disease panorama and challenges","volume":"183","author":"Stamm","year":"2001","journal-title":"J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1086\/511866","article-title":"Adherence to the Infectious Diseases Society of America guidelines in the treatment of uncomplicated urinary tract infection","volume":"44","author":"Taur","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_3","first-page":"1","article-title":"Ambulatory care visits to physician offices, hospital outpatient departments, and emergency de-partments: United States, 2001\u20132002","volume":"13","author":"Schappert","year":"2006","journal-title":"Vital. Health Stat."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"592","DOI":"10.4161\/viru.22364","article-title":"Molecular blueprint of uropathogenic Escherichia coli virulence provides clues toward the development of anti-virulence therapeutics","volume":"3","author":"Kostakioti","year":"2012","journal-title":"Virulence"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1097\/QCO.0000000000000228","article-title":"Global epidemiology of urinary tract infections","volume":"29","author":"Tandogdu","year":"2016","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_6","first-page":"103","article-title":"Prevalence and predictors of antibiotic prescription errors in an emergency de-partment, Central Saudi Arabia","volume":"7","author":"Alanazi","year":"2015","journal-title":"Drug Healthc. Patient Saf."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1136\/bmj.332.7533.94","article-title":"Urinary tract infections in women: Diagnosis and management in primary care","volume":"332","author":"Car","year":"2006","journal-title":"BMJ"},{"key":"ref_8","unstructured":"Michael, S. (2013). Chapter 9\u2014Uropathogenic Escherichia coli. Donnenberg, Escherichia coli, Elsevier Inc., Academic Press. [2nd ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.2147\/TCRM.S178855","article-title":"An evaluation of community-acquired urinary tract infection and appropriateness of treatment in an emergency department in Saudi Arabia","volume":"14","author":"Alanazi","year":"2018","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_10","first-page":"326","article-title":"Urinary tract infection. Diabetics and non-diabetic patients","volume":"22","author":"Akbar","year":"2001","journal-title":"Saudi Med. J."},{"key":"ref_11","unstructured":"General Authority for Statistics (2022, May 03). Statistical Yearbook of 2016, Kingdom of Saudi Arabia: General Authority for Statistics, Available online: https:\/\/www.stats.gov.sa\/en\/169."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"497","DOI":"10.15537\/smj.2015.4.10770","article-title":"Urinary tract infection in children younger than 5 years. Etiology and asso-ciated urological anomalies","volume":"36","author":"Garout","year":"2015","journal-title":"Saudi Med. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13756-015-0085-0","article-title":"Multidrug resistant Enterobacteriaceae and extended spectrum \u03b2-lactamase producing Escherichia coli: A cross-sectional study in National Kidney Center, Nepal","volume":"4","author":"Yadav","year":"2015","journal-title":"Antimicrob Resist. Infect Control."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1038\/s41598-020-79791-0","article-title":"Comprehensive study of antimicrobial suscep-tibility pattern and extended spectrum beta-lactamase (ESBL) prevalence in bacteria isolated from urine samples","volume":"11","author":"Gharavi","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"157","DOI":"10.18683\/germs.2020.1201","article-title":"A retrospective cross-sectional study of urinary tract infections and prevalence of antibiotic resistant pathogens in patients with diabetes mellitus from a public hospital in Surabaya, Indonesia","volume":"10","author":"Norafika","year":"2020","journal-title":"Germs"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"650","DOI":"10.3390\/clinpract11030080","article-title":"Antimicrobial Resistance Patterns and ESBL of Uropathogens Isolated from Adult Females in Najran Region of Saudi Arabia","volume":"11","author":"Alasmary","year":"2021","journal-title":"Clin. Pract."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3026851","DOI":"10.1155\/2018\/3026851","article-title":"Prevalence of Multidrug Resistance and Extended-Spectrum \u03b2-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia","volume":"2018","author":"Alqasim","year":"2018","journal-title":"Int. J. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"681473","DOI":"10.1155\/2012\/681473","article-title":"Role of Uropathogenic Escherichia coli Virulence Factors in Development of Urinary Tract Infection and Kidney Damage","volume":"2012","author":"Bien","year":"2012","journal-title":"Int. J. Nephrol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"eaaf1283","DOI":"10.1126\/scitranslmed.aaf1283","article-title":"Bacterial virulence phenotypes of Escherichia coli and host susceptibility determine risk for urinary tract infections","volume":"9","author":"Schreiber","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"ref_20","first-page":"e9936","article-title":"The Association of Virulence Determinants of Uropathogenic Escherichia coli With Antibiotic Resistance","volume":"7","author":"Asadi","year":"2014","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Shah, C., Baral, R., Bartaula, B., and Shrestha, L.B. (2019). Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1587-3"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1093\/jac\/dkv188","article-title":"Antibiotic-resistant ST38, ST131 and ST405 strains are the leading uropathogenic Escherichia coli clones in Riyadh, Saudi Arabia","volume":"70","author":"Alghoribi","year":"2015","journal-title":"J. Antimicrob. Chemother"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1086\/377455","article-title":"Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and\/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans","volume":"188","author":"Johnson","year":"2003","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.sjbs.2019.09.020","article-title":"Prevalence and molecular characteristics of sequence type 131 clone among clinical uropathogenic Escherichia coli isolates in Riyadh, Saudi Arabia","volume":"27","author":"Alqasim","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.2147\/IDR.S299846","article-title":"Antimicrobial Surveillance for Bacterial Uropathogens in Hail, Saudi Arabia: A Five-Year Multi-center Retrospective Study","volume":"14","author":"Bazaid","year":"2021","journal-title":"Infect. Drug Resist."},{"key":"ref_26","first-page":"393","article-title":"Clinical and Laboratory Profile of Urinary Tract Infections Associated with Extended Spectrum \u03b2-Lactamase Producing Escherichia coli and Klebsiella pneumoniae","volume":"46","author":"Younis","year":"2016","journal-title":"Ann. Clin. Lab Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1159\/000198698","article-title":"Antimicrobial susceptibility pattern of bacterial pathogens causing urinary tract infections in a Saudi Arabian hospital","volume":"55","author":"Anani","year":"2009","journal-title":"Chemotherapy"},{"key":"ref_28","first-page":"854","article-title":"Antibiotic resistance pattern and empirical therapy for urinary tract infections in children","volume":"29","year":"2008","journal-title":"Saudi Med. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"239","DOI":"10.5144\/0256-4947.2005.239","article-title":"Prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing Esche-richia coli and Klebsiella pneumoniae in a general hospital","volume":"25","author":"Kader","year":"2005","journal-title":"Ann. Saudi. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"261","DOI":"10.5144\/0256-4947.1988.261","article-title":"Urinary tract infection at a University Hospital in Saudi Arabia: Incidence, microbiology, and antimicrobial susceptibility","volume":"8","author":"Eltahawy","year":"1988","journal-title":"Ann. Saudi Med."},{"key":"ref_31","unstructured":"CLSI (2020). Performance Standards for Antimicrobial Susceptibility Testing, CLSI supplement M100; Clinical and La-bor-atory Standards Institute. [30th ed.]. Available online: https:\/\/www.nih.org.pk\/wp-content\/uploads\/2021\/02\/CLSI2020.pdf."},{"key":"ref_32","unstructured":"Clinical Laboratory Standards Institute (2012). Methods for Dilution Antimicrobial Susceptibility Testing for Bacteria That Grows Aerobically, Clinical Laboratory Standards Institute. [9th ed.]. (M07-A9)."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"70","DOI":"10.4103\/0974-2727.72152","article-title":"An approach to uropathogenic Escherichia coli in urinary tract infec-tions","volume":"2","author":"Ranjan","year":"2010","journal-title":"J. Lab Physicians"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1099\/00222615-41-2-127","article-title":"Virulence-associated factors in Escherichia coli strains isolated from children with urinary tract infections","volume":"41","author":"Siegfried","year":"1994","journal-title":"J. Med. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/AEM.66.10.4555-4558.2000","article-title":"Rapid and simple determination of the Escherichia coli phylogenetic group","volume":"66","author":"Clermont","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1128\/jcm.30.5.1189-1193.1992","article-title":"Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction","volume":"30","author":"Archambaud","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1574-695X.1995.tb00179.x","article-title":"Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction","volume":"12","author":"Yamamoto","year":"1995","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1086\/315217","article-title":"Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise","volume":"181","author":"Johnson","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1128\/IAI.68.5.3040-3047.2000","article-title":"Molecular epidemiological and phylogenetic associations of two novel putative virulence genes, iha and iroN (E. coli), among Escherichia coli isolates from patients with urosepsis","volume":"68","author":"Johnson","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Dadi, B.R., Abebe, T., Zhang, L., Mihret, A., Abebe, W., and Amogne, W. (2020). Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia. BMC Infect. Dis., 20.","DOI":"10.1186\/s12879-020-4844-z"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Mazumder, R., Abdullah, A., Ahmed, D., and Hussain, A. (2020). High Prevalence of blaCTX-M-15 Gene among Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli Isolates Causing Extraintestinal Infections in Bangladesh. Antibiotics, 9.","DOI":"10.3390\/antibiotics9110796"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/lam.12517","article-title":"Phylogenetic group distributions, virulence factors and antimicrobial resistance properties of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in South Korea","volume":"62","author":"Lee","year":"2016","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"846219","DOI":"10.1155\/2015\/846219","article-title":"Phylogenetic groups of Escherichia coli strains from patients with urinary tract infection in Iran based on the new Clermont phylotyping method","volume":"2015","author":"Iranpour","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Al-Zahrani, J., Al Dossari, K., Gabr, A.H., Ahmed, A.-F., Al Shahrani, S.A., and Al-Ghamdi, S. (2019). Antimicrobial resistance patterns of Uropathogens isolated from adult women with acute uncomplicated cystitis. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1612-6"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"946","DOI":"10.3855\/jidc.10553","article-title":"The antibiotic susceptibility patterns of uropathogens isolated in Qassim, Saudi Arabia","volume":"12","author":"Adam","year":"2018","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"570238","DOI":"10.3389\/fphar.2020.570238","article-title":"Evaluation of a Multidisciplinary Antimicrobial Stewardship Program in a Saudi Critical Care Unit: A Quasi-Experimental Study","volume":"11","author":"Haseeb","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s13756-020-00751-4","article-title":"The impact of antimicrobial stewardship program implementation at four tertiary private hospitals: Results of a five-years pre-post analysis","volume":"9","author":"Alhumaid","year":"2020","journal-title":"Antimicrob Resist. Infect. Control"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"935","DOI":"10.15537\/smj.2016.9.16139","article-title":"Antimicrobial resistance in Saudi Arabia. An urgent call for an immediate action","volume":"37","author":"Zowawi","year":"2016","journal-title":"Saudi Med. J."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Alghamdi, S., Berrou, I., Aslanpour, Z., Mutlaq, A., Haseeb, A., Albanghali, M., Hammad, M.A., and Shebl, N. (2021). Antimicrobial Stewardship Programmes in Saudi Hospitals: Evidence from a National Survey. Antibiotics, 10.","DOI":"10.3390\/antibiotics10020193"},{"key":"ref_50","first-page":"e14981","article-title":"Public Perception Toward Ministry of Health Regulations for Antibiotic Dispensing and Its Impact on Pharmacy and Family Physician Visits","volume":"13","author":"AlOtieschan","year":"2021","journal-title":"Cureus"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.eursup.2016.04.003","article-title":"Treatment of urinary tract infections and antibiotic stewardship","volume":"15","author":"Bartoletti","year":"2016","journal-title":"Eur. Urol. Suppl."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"403","DOI":"10.33073\/pjm-2019-048","article-title":"Antibiotic Resistance Among Uropathogenic Escherichia coli","volume":"68","author":"Kot","year":"2019","journal-title":"Pol. J. Microbiol"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1007\/s11033-018-4254-0","article-title":"The genetic background of antibiotic resistance among clinical uropathogenic Escherichia coli strains","volume":"45","author":"Baraniak","year":"2018","journal-title":"Mol. Biol. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2174\/1874279301810010079","article-title":"Antimicrobial resistance of uropathogenic Escherichia coli from elderly patients at a general hospital, Argentina","volume":"10","author":"Delpech","year":"2018","journal-title":"Open Infect. Dis. J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"63.e1","DOI":"10.1016\/j.cmi.2015.08.019","article-title":"Comparative in vitro activity of oral antimicrobial agents against Enterobacteriaceae from patients with community acquired urinary tract infections in three European countries","volume":"22","author":"Kresken","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1590\/S1678-9946201658002","article-title":"Antibiotic resistance patterns of urinary tract infections in a north-eastern Brazilian capital","volume":"58","author":"Cunha","year":"2016","journal-title":"Rev. Inst. Med. Trop S\u00e3o Paulo"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.jhin.2016.12.008","article-title":"coli bacteraemia sentinel surveillance group. Epidemiology of Escherichia coli bacteraemia in England: Results of an enhanced sentinel surveil-lance programme","volume":"95","author":"Abernethy","year":"2017","journal-title":"J. Hosp. Infect."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"416","DOI":"10.5455\/msm.2016.28.416-419","article-title":"Antibiotic Resistance in Urinary Isolates of Escherichia coli","volume":"28","author":"Abduzaimovic","year":"2016","journal-title":"Mater Sociomed"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e01834\u2013e17","DOI":"10.1128\/JCM.01834-17","article-title":"Persistent Pandemic Lineages of Uropathogenic Escherichia coli in a College Community from 1999 to 2017","volume":"56","author":"Yamaji","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.2147\/IDR.S201849","article-title":"Changing antibiotic susceptibility pattern in uropathogenic Escherichia coli over a period of 5 years in a tertiary care center","volume":"12","author":"Prasada","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"5744","DOI":"10.1128\/AAC.00402-12","article-title":"Experience with fosfomycin for treatment of urinary tract infections due to multidrug-resistant organisms","volume":"56","author":"Neuner","year":"2012","journal-title":"Antimicrob. Agents Chemother"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"220","DOI":"10.3855\/jidc.9517","article-title":"Antimicrobial susceptibility of microorganisms causing Urinary Tract Infections in Saudi Arabia","volume":"12","author":"Balkhi","year":"2018","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12941-016-0177-6","article-title":"The occurrence of ESBL-producing Escherichia coli carrying aminoglycoside resistance genes in urinary tract infections in Saudi Arabia","volume":"16","author":"Alyamani","year":"2017","journal-title":"Ann. Clin. Microbiol. An-timicrob."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/j.1469-0691.2007.01855.x","article-title":"Prevalence and clonality of extended-spectrum beta-lactamases in Asia","volume":"14","author":"Hawkey","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1582-4934.2001.tb00164.x","article-title":"Prevalence of virulence genes in Escherichia coli strains isolated from Romanian adult urinary tract infection cases","volume":"5","author":"Usein","year":"2001","journal-title":"J. Cell Mol. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/ja.2014.72","article-title":"Drug resistance and virulence of uropa-tho-genic Escherichia coli from Shanghai, China","volume":"67","author":"Wang","year":"2014","journal-title":"J. Antibiot (Tokyo)"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3855\/jidc.7903","article-title":"Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia","volume":"11","author":"Munkhdelger","year":"2017","journal-title":"J. Infect. Dev. Ctries"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Khairy, R.M., Mohamed, E.S., Ghany, H.M.A., and Abdelrahim, S.S. (2019). Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrheagenic E. coli strains isolated from community acquired infections. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222441"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"485","DOI":"10.11622\/smedj.2016153","article-title":"Urinary tract infections in adults","volume":"57","author":"Tan","year":"2016","journal-title":"Singapore Med. J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1476-0711-12-8","article-title":"Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties","volume":"12","author":"Momtaz","year":"2013","journal-title":"Ann. Clin. Microbiol. Antimicrob"},{"key":"ref_71","first-page":"DC01","article-title":"Genotypic Characterization of Virulence Factors in Escherichia coli Isolated from Patients with Acute Cystitis, Pyelonephritis and Asymptomatic Bacteriuria","volume":"10","author":"Tabasi","year":"2016","journal-title":"J. Clin. Diagn Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e450","DOI":"10.1016\/j.ijid.2013.01.025","article-title":"Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection","volume":"17","author":"Tarchouna","year":"2013","journal-title":"Int. J. Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.yexmp.2008.03.007","article-title":"Origins and virulence mechanisms of uropathogenic Escherichia coli","volume":"85","author":"Wiles","year":"2008","journal-title":"Exp. Mol. Pathol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/6\/631\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:22:10Z","timestamp":1760138530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/6\/631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,30]]},"references-count":73,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["pathogens11060631"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11060631","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,30]]}}}