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(R.O.C.)","doi-asserted-by":"publisher","award":["NMRPG6K6011-12"],"award-info":[{"award-number":["NMRPG6K6011-12"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Periprosthetic joint infection (PJI) is a troublesome clinical issue in total joint arthroplasty (TJA). Although methicillin-resistant Staphylococcus aureus (MRSA) is considered to be the most serious pathogen in PJIs, little is known about the genotypic and phenotypic characteristics of MRSA clones isolated from PJI patients. A total of 36 MRSA isolates from PJI patients were collected at the Chang-Gung Memorial Hospital in Taiwan from May 2016 to October 2019. All MRSA isolates were subjected to genome typing. The prevalence of Panton\u2013Valentine leucocidin (PVL), the antibiotic susceptibility profile, and the biofilm formation ability were compared among different MRSA genogroups. Additionally, demographics and clinical manifestations of patients infected with different MRSA genogroups were investigated. Eight sequence types (STs) were identified among 36 isolated from PJIs. According to the incidence of MRSA genotypes in PJIs, in this study, we divided them into four groups, including ST8 (n = 10), ST59 (n = 8), ST239 (n = 11), and other STs (n = 7). For the antibiotic susceptibility testing, we found that all MRSA isolates in the ST239 group were highly resistant to ciprofloxacin, gentamicin trimethoprim-sulfamethoxazole, and levofloxacin. Additionally, ST239 MRSA also had a higher ability to form biofilm than other groups. Importantly, patients with ST239 infection typically had a fever and exhibited higher levels of inflammatory markers, including C-reactive protein (CRP) and white blood cell count (WBC). Epidemiological investigations revealed that knee PJIs were mainly attributed to infection with ST59 MRSA and increasing trends for infection with ST8 and other ST types of MRSAs in PJI patients were observed from 2016 to 2019. The identification of MRSA genotypes in PJIs may be helpful for the management of PJIs.<\/jats:p>","DOI":"10.3390\/pathogens11070719","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T22:43:00Z","timestamp":1656024180000},"page":"719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Periprosthetic Joint Infections"],"prefix":"10.3390","volume":"11","author":[{"given":"Jiun-Liang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-5631","authenticated-orcid":false,"given":"Tsung-Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Medicine, Chang Gung University, Taoyuan 33303, Taiwan"},{"name":"Division of Infectious Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"},{"name":"Department of Nursing, Chang Gung University of Science and Technology, Chiayi Campus, Chiayi 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Bin","family":"Hsu","sequence":"additional","affiliation":[{"name":"Sports Medicine Center, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiang-Wen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nursing, Division of Basic Medical Sciences, Recurrent Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Puzi City 61363, Taiwan"},{"name":"Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Guishan Dist., Taoyuan 33303, Taiwan"},{"name":"Department of Rehabilitation, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7619-1902","authenticated-orcid":false,"given":"Yao-Chang","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Nursing, Division of Basic Medical Sciences, Recurrent Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Puzi City 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6492-0346","authenticated-orcid":false,"given":"Pey-Jium","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang-Gung University, Taoyuan 33302, Taiwan"},{"name":"Department of Nephrology, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Ti","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Chiayi 61363, Taiwan"},{"name":"College of Medicine, Chang Gung University, Taoyuan 33303, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1016\/S0140-6736(12)60607-2","article-title":"Hip arthroplasty","volume":"380","author":"Pivec","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.2106\/JBJS.N.01141","article-title":"Prevalence of Total Hip and Knee Replacement in the United States","volume":"97","author":"Larson","year":"2015","journal-title":"J. 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