{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:43:00Z","timestamp":1760229780181,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-1067"],"award-info":[{"award-number":["075-15-2021-1067"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The mature serine-type IgA1 protease from Neisseria meningitidis serogroup B strain H44\/76 (IgA1pr1_28\u20131004) is considered here as the basis for creating a candidate vaccine against meningococcal meningitis. In this work, we examine the primary structure similarity of IgA1 proteases from various strains of a number of Gram-negative bacteria (N. meningitidis, Neisseria gonorrhoeae, Haemophilus influenzae) in order to find a structural groundwork for creating a broad-spectrum vaccine based on fragments of this enzyme. BLAST has shown high similarity between the primary structure of IgA1pr1_28\u20131004 and hypothetical sequences of mature IgA1 proteases from N. meningitidis (in 1060 out of 1061 examined strains), N. gonorrhoeae (in all 602 examined strains) and H. influenzae (in no less than 137 out of 521 examined strains). For these enzymes, common regions of sequence correspond to IgA1pr1_28\u20131004 fragments 28\u201384, 146\u2013193, 253\u2013539, 567\u2013628, 639\u2013795 and 811\u20131004, with identity of at least 85%. We believe that these fragments can be used in the development of a vaccine to prevent diseases caused by pathogenic strains of N. meningitidis and N. gonorrhoeae as well as a significant number of strains of H. influenzae.<\/jats:p>","DOI":"10.3390\/pathogens11070734","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T01:48:38Z","timestamp":1656467318000},"page":"734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Highly Similar Sequences of Mature IgA1 Proteases from Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7100-6432","authenticated-orcid":false,"given":"David","family":"Karlinsky","sequence":"first","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri","family":"Prokopenko","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexei","family":"Zinchenko","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa","family":"Zhigis","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Kotelnikova","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lev","family":"Rumsh","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0384-6568","authenticated-orcid":false,"given":"Ivan","family":"Smirnov","sequence":"additional","affiliation":[{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, 16\/10, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1016\/B978-0-7020-4088-7.00093-6","article-title":"Bacterial meningitis","volume":"121","author":"Heckenberg","year":"2014","journal-title":"Handb. Clin. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.biocel.2005.10.005","article-title":"IgA1 protease","volume":"38","author":"Mistry","year":"2006","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1128\/IAI.66.1.181-190.1998","article-title":"A Comprehensive Genetic Study of Streptococcal Immunoglobulin A1 Proteases: Evidence for Recombination within and between Species","volume":"66","author":"Poulsen","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1099\/mic.0.082511-0","article-title":"Variable processing of the IgA protease autotransporter at the cell surface of Neisseria meningitidis","volume":"160","author":"Arenas","year":"2014","journal-title":"Microbiology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1128\/IAI.01671-06","article-title":"Relaxed Cleavage Specificity of an Immunoglobulin A1 Protease from Neisseria meningitidis","volume":"75","author":"Vitovski","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1111\/j.1365-2958.1995.tb02263.x","article-title":"Comparative characterization of the iga gene encoding IgA1 protease in Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae","volume":"15","author":"Lomholt","year":"1995","journal-title":"Mol. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1001\/jama.2012.85","article-title":"Immunogenicity and Tolerability of Recombinant Serogroup B Meningococcal Vaccine Administered With or Without Routine Infant Vaccinations According to Different Immunization Schedules: A Randomized Controlled Trial","volume":"307","author":"Gossger","year":"2012","journal-title":"JAMA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S54","DOI":"10.1086\/648966","article-title":"Review of Meningococcal Group B Vaccines","volume":"50","author":"Granoff","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.4161\/hv.24129","article-title":"Vaccines against meningococcal serogroup B disease containing outer membrane vesicles (OMV): Lessons from past programs and implications for the future","volume":"9","author":"Holst","year":"2013","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1134\/S106816202104021X","article-title":"IgA1 Protease as a Vaccine Basis for Prevention of Bacterial Meningitis","volume":"47","author":"Zhigis","year":"2021","journal-title":"Russ. J. Bioorg. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1134\/S1068162010010085","article-title":"Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis","volume":"36","author":"Yagudaeva","year":"2010","journal-title":"Russ. J. Bioorg. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1134\/S1990750813040033","article-title":"Protective properties of recombinant IgA1 protease from meningococcus","volume":"7","author":"Alliluev","year":"2013","journal-title":"Biochem. Mosc. Suppl. Ser. B Biomed. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.micinf.2019.02.003","article-title":"Protective potency of recombinant meningococcal IgA1 protease and its structural derivatives upon animal invasion with meningococcal and pneumococcal infections","volume":"21","author":"Kotelnikova","year":"2019","journal-title":"Microbes Infect."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1134\/S1068162018010193","article-title":"Immunogenic and Protective Properties of Neisseria meningitidis IgA1 Protease and of Its Truncated Fragments","volume":"44","author":"Zinchenko","year":"2018","journal-title":"Russ. J. Bioorg. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10517-016-3422-2","article-title":"Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins","volume":"161","author":"Kotelnikova","year":"2016","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.micinf.2015.12.005","article-title":"Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2","volume":"18","author":"Fu","year":"2016","journal-title":"Microbes Infect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1111\/j.1742-4658.2005.04945.x","article-title":"Protein database searches using compositionally adjusted substitution matrices","volume":"272","author":"Altschul","year":"2005","journal-title":"FEBS J."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"The UniProt Consortium, Bateman, A., Martin, M.-J., Orchard, S., Magrane, M., Agivetova, R., Ahmad, S., Alpi, E., Bowler-Barnett, E.H., and Britto, R. (2021). UniProt: The universal protein knowledgebase in 2021. Nucleic Acids Res., 49, D480\u2013D489.","DOI":"10.1093\/nar\/gkaa1100"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"256","DOI":"10.3389\/fcimb.2017.00256","article-title":"Biological Functions of the Secretome of Neisseria meningitidis","volume":"7","author":"Tommassen","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3389\/fcimb.2011.00005","article-title":"Structure and function of the Haemophilus influenzae autotransporters","volume":"1","author":"Spahich","year":"2011","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1093\/infdis\/jiv299","article-title":"Expression of IgA Proteases by Haemophilus influenzae in the Respiratory Tract of Adults With Chronic Obstructive Pulmonary Disease","volume":"212","author":"Murphy","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: Massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"Varadi","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"D10","DOI":"10.1093\/nar\/gkaa892","article-title":"Database resources of the National Center for Biotechnology Information","volume":"49","author":"Sayers","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"D733","DOI":"10.1093\/nar\/gkv1189","article-title":"Reference sequence (RefSeq) database at NCBI: Current status, taxonomic expansion, and functional annotation","volume":"44","author":"Wright","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"D106","DOI":"10.1093\/nar\/gkab1051","article-title":"The European Nucleotide Archive in 2021","volume":"50","author":"Cummins","year":"2022","journal-title":"Nucleic Acids Res."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/7\/734\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:39:28Z","timestamp":1760139568000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/7\/734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,28]]},"references-count":27,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["pathogens11070734"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11070734","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2022,6,28]]}}}