{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:39:09Z","timestamp":1788881949669,"version":"build-2803163510"},"reference-count":76,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia (FCT)","award":["PTDC\/CVT-CVT\/29073\/2017"],"award-info":[{"award-number":["PTDC\/CVT-CVT\/29073\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25\/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoGBartonella DNA in sampled cats ranged from 2.78 \u00d7 10 to 1.03 \u00d7 105 copies\/\u00b5L. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.<\/jats:p>","DOI":"10.3390\/pathogens11070749","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T01:40:36Z","timestamp":1656639636000},"page":"749","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal"],"prefix":"10.3390","volume":"11","author":[{"given":"Estefania","family":"Torrej\u00f3n","sequence":"first","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Seron","family":"Sanches","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Escola de Ci\u00eancias da Vida, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, Rua Imaculada Concei\u00e7\u00e3o 1155, Curitiba 80215-901, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7841-3938","authenticated-orcid":false,"given":"Leonardo","family":"Moerbeck","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenira","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Veterin\u00e1ria, Universidade Estadual Paulista (FCAV-UNESP), Via de Acesso Paulo Donato Castellane, s\/n, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4570-2060","authenticated-orcid":false,"given":"Ana","family":"Domingos","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5512-9093","authenticated-orcid":false,"given":"Sandra","family":"Antunes","sequence":"additional","affiliation":[{"name":"Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"},{"name":"Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1476-4431.2009.00496.x","article-title":"Bartonellosis: An Emerging Infectious Disease of Zoonotic Importance to Animals and Human Beings","volume":"20","author":"Breitschwerdt","year":"2010","journal-title":"J. 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