{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:43:32Z","timestamp":1791333812812,"version":"4.3.1"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior, Brasil (CAPES)","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"DOI":"10.13039\/501100004901","name":"FAPEMIG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"FAPEMIG (Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais)","doi-asserted-by":"publisher","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CNPq","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CNPq","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"Brazilian Ministry of Health (SVS-DEIDT and SCTIE-DECIT)","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Brazilian Ministry of Health (SVS-DEIDT and SCTIE-DECIT)","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]},{"name":"INCT-EM","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"INCT-EM","award":["APQ-02760-17"],"award-info":[{"award-number":["APQ-02760-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Arboviruses (an acronym for \u201carthropod-borne virus\u201d), such as dengue, yellow fever, Zika, and Chikungunya, are important human pathogens transmitted by mosquitoes. These viruses impose a growing burden on public health. Despite laboratory mice having been used for decades for understanding the basic biological phenomena of these viruses, it was only recently that researchers started to develop immunocompromised animals to study the pathogenesis of arboviruses and their transmission in a way that parallels natural cycles. Here, we show that the AG129 mouse (IFN \u03b1\/\u03b2\/\u03b3 R\u2212\/\u2212) is a suitable and comprehensive vertebrate model for studying the mosquito vector competence for the major arboviruses of medical importance, namely the dengue virus (DENV), yellow fever virus (YFV), Zika virus (ZIKV), Mayaro virus (MAYV), and Chikungunya virus (CHIKV). We found that, after intraperitoneal injection, AG129 mice developed a transient viremia lasting several days, peaking on day two or three post infection, for all five arboviruses tested in this study. Furthermore, we found that the observed viremia was ample enough to infect Aedes aegypti during a blood meal from the AG129 infected mice. Finally, we demonstrated that infected mosquitoes could transmit each of the tested arboviruses back to na\u00efve AG129 mice, completing a full transmission cycle of these vector-borne viruses. Together, our data show that A129 mice are a simple and comprehensive vertebrate model for studies of vector competence, as well as investigations into other aspects of mosquito biology that can affect virus\u2013host interactions.<\/jats:p>","DOI":"10.3390\/pathogens11080879","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T23:33:01Z","timestamp":1659569581000},"page":"879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["AG129 Mice as a Comprehensive Model for the Experimental Assessment of Mosquito Vector Competence for Arboviruses"],"prefix":"10.3390","volume":"11","author":[{"given":"L\u00edvia V. R.","family":"Baldon","sequence":"first","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana F.","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via V.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda O.","family":"Rezende","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siad C. G.","family":"Amadou","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627-Pampulha, Belo Horizonte 31270-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3286-2011","authenticated-orcid":false,"given":"Thiago H. J. F.","family":"Leite","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627-Pampulha, Belo Horizonte 31270-901, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcele N.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3457-3320","authenticated-orcid":false,"given":"Jo\u00e3o T.","family":"Marques","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627-Pampulha, Belo Horizonte 31270-901, Brazil"},{"name":"Facult\u00e9 des Sciences de la Vie, Universit\u00e9 de Strasbourg, CNRS UPR9022, Inserm U1257, 67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano A.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Mosquitos Vetores: Endossimbiontes e Intera\u00e7\u00e3o Pat\u00f3geno-Vetor, Instituto Ren\u00e9 Rachou-Fiocruz, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro G. 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