{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:23:34Z","timestamp":1788344614364,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The School of Veterinary Medicine, Louisiana State University","award":["PG009641"],"award-info":[{"award-number":["PG009641"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Feline infectious peritonitis (FIP) virus is the most common infectious cause of uveitis in cats. Confirmatory diagnosis is usually only reached at postmortem examination. The relationship between the histologic inflammatory pattern, which depends on the stage of the disease, and the likelihood of detection of the viral antigen and\/or RNA has not been investigated. We hypothesized that viral detection rate by either immunohistochemistry, in situ hybridization or RT-qPCR is dependent upon the predominant type of uveal inflammatory response (i.e., pyogranulomatous vs. plasmacytic). Thus, the aims of this study were to evaluate cases of FIP-induced uveitis, localize the viral antigen and RNA, and assess the relationship between the inflammatory pattern (macrophage- vs. plasma cell-rich) and the likelihood of detecting the FIP antigen and\/or RNA. We evaluated 30 cats with FIP-induced uveitis. The viral antigen and\/or RNA were detected within uveal macrophages in 11\/30 cases, of which 8 tested positive by RT-qPCR. Correlation analysis determined a weak to moderate but significant negative correlation between the degree of plasmacytic uveal inflammation and the likelihood of detecting the FIP antigen and RNA. This study suggests that predominance of plasmacytic inflammation in cases of FIP uveitis reduces the odds of a confirmatory diagnosis through the viral detection methods available.<\/jats:p>","DOI":"10.3390\/pathogens11080883","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T04:16:55Z","timestamp":1660018615000},"page":"883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Relationship between Uveal Inflammation and Viral Detection in 30 Cats with Feline Infectious Peritonitis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3864-5915","authenticated-orcid":false,"given":"Mariano","family":"Carossino","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Del Piero","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongha","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7215-5306","authenticated-orcid":false,"given":"David B.","family":"Needle","sequence":"additional","affiliation":[{"name":"New Hampshire Veterinary Diagnostic Laboratory, College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH 03824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan M.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77845, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald R.","family":"Riis","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Maes","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel G.","family":"Wise","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keenan","family":"Mullaney","sequence":"additional","affiliation":[{"name":"Washtenaw Technical Middle College, Ann Arbor, MI 48105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Ferracone","sequence":"additional","affiliation":[{"name":"PennVet New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-4391","authenticated-orcid":false,"given":"Ingeborg M.","family":"Langohr","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/S0195-5616(00)05002-6","article-title":"Feline infectious peritonitis","volume":"30","author":"Andrew","year":"2000","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.3201\/eid1509.081573","article-title":"Genetics and pathogenesis of feline infectious peritonitis virus","volume":"15","author":"Brown","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1099\/vir.0.016485-0","article-title":"Feline infectious peritonitis: Insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene","volume":"91","author":"Chang","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1177\/0300985814522077","article-title":"Feline infectious peritonitis: Still an enigma?","volume":"51","author":"Kipar","year":"2014","journal-title":"Vet. Pathol."},{"key":"ref_5","first-page":"609465","article-title":"Feline and canine coronaviruses: Common genetic and pathobiological features","volume":"2011","year":"2011","journal-title":"Adv. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jfms.2008.09.008","article-title":"A review of feline infectious peritonitis virus infection: 1963\u20132008","volume":"11","author":"Pedersen","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"14122","DOI":"10.1128\/JVI.79.22.14122-14130.2005","article-title":"Acquisition of macrophage tropism during the pathogenesis of feline infectious peritonitis is determined by mutations in the feline coronavirus spike protein","volume":"79","author":"Rottier","year":"2005","journal-title":"J. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.virusres.2010.12.020","article-title":"Mutation of neutralizing\/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: Influences of viral replication in monocytes\/macrophages and virulence in cats","volume":"156","author":"Takano","year":"2011","journal-title":"Virus Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.virusres.2011.04.031","article-title":"Intriguing interplay between feline infectious peritonitis virus and its receptors during entry in primary feline monocytes","volume":"160","author":"Desmarets","year":"2011","journal-title":"Virus Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"109849","DOI":"10.1155\/2011\/109849","article-title":"The paradox of feline coronavirus pathogenesis: A review","volume":"2011","author":"Myrrha","year":"2011","journal-title":"Adv. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/j.cvsm.2011.08.004","article-title":"Feline coronavirus in multicat environments","volume":"41","author":"Drechsler","year":"2011","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1053\/j.ctsap.2004.12.016","article-title":"Feline uveitis: Diagnosis and treatment","volume":"20","author":"Colitz","year":"2005","journal-title":"Clin. Tech. Small Anim. Pract."},{"key":"ref_13","first-page":"417","article-title":"Ocular manifestations of feline infectious peritonitis","volume":"159","author":"Doherty","year":"1971","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/vop.12324","article-title":"Causes of endogenous uveitis in cats presented to referral clinics in North Carolina","volume":"19","author":"Jinks","year":"2016","journal-title":"Vet. Ophthalmol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"135","DOI":"10.2460\/javma.1991.198.01.135","article-title":"Histopathologic study of uveitis in cats: 139 cases (1978\u20131988)","volume":"198","author":"Peiffer","year":"1991","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1136\/vr.129.10.209","article-title":"Feline infectious peritonitis: A review of clinicopathological changes in 65 cases, and a critical assessment of their diagnostic value","volume":"129","author":"Sparkes","year":"1991","journal-title":"Vet. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jfms.2010.09.014","article-title":"Clinicopathological findings and disease staging of feline infectious peritonitis: 51 cases from 2003 to 2009 in Taiwan","volume":"13","author":"Tsai","year":"2011","journal-title":"J. Feline Med. Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1177\/0300985817728557","article-title":"Feline Infectious Peritonitis: Immunohistochemical Features of Ocular Inflammation and the Distribution of Viral Antigens in Structures of the Eye","volume":"54","author":"Ziolkowska","year":"2017","journal-title":"Vet. Pathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1177\/1040638718755833","article-title":"A retrospective study of the neuropathology and diagnosis of naturally occurring feline infectious peritonitis","volume":"30","author":"Rissi","year":"2018","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1177\/1098612X18762883","article-title":"Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls","volume":"21","author":"Sangl","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1177\/1040638718756460","article-title":"Comparison of the performance of laboratory tests in the diagnosis of feline infectious peritonitis","volume":"30","author":"Stranieri","year":"2018","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Stranieri, A., Scavone, D., Paltrinieri, S., Giordano, A., Bonsembiante, F., Ferro, S., Gelain, M.E., Meazzi, S., and Lauzi, S. (2020). Concordance between Histology, Immunohistochemistry, and RT-PCR in the Diagnosis of Feline Infectious Peritonitis. Pathogens, 9.","DOI":"10.3390\/pathogens9100852"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1177\/1098612X18758592","article-title":"Diagnosis of feline infectious peritonitis: Update on evidence supporting available tests","volume":"20","author":"Tasker","year":"2018","journal-title":"J. Feline Med. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Dubielzig, R.R., Ketring, K., McLellan, G.J., and Albert, D.M. (2010). The uvea. Veterinary Ocular Pathology: A Comparative Review, Elsevier.","DOI":"10.1016\/B978-0-7020-2797-0.00009-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1128\/jvi.66.11.6695-6705.1992","article-title":"Monoclonal antibody analysis of neutralization and antibody-dependent enhancement of feline infectious peritonitis virus","volume":"66","author":"Corapi","year":"1992","journal-title":"J. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.cvsm.2020.05.002","article-title":"Feline Infectious Peritonitis: Update on Pathogenesis, Diagnostics, and Treatment","volume":"50","author":"Kennedy","year":"2020","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1099\/0022-1317-74-4-745","article-title":"Identification of antigenic sites mediating antibody-dependent enhancement of feline infectious peritonitis virus infectivity","volume":"74","author":"Olsen","year":"1993","journal-title":"J. Gen. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1128\/jvi.66.2.956-965.1992","article-title":"Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages","volume":"66","author":"Olsen","year":"1992","journal-title":"J. Virol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.virusres.2011.12.020","article-title":"Feline infectious peritonitis: Role of the feline coronavirus 3c gene in intestinal tropism and pathogenicity based upon isolates from resident and adopted shelter cats","volume":"165","author":"Pedersen","year":"2012","journal-title":"Virus Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s00705-009-0371-3","article-title":"Neutrophil survival factors (TNF-alpha, GM-CSF, and G-CSF) produced by macrophages in cats infected with feline infectious peritonitis virus contribute to the pathogenesis of granulomatous lesions","volume":"154","author":"Takano","year":"2009","journal-title":"Arch. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.virusres.2011.03.027","article-title":"Vascular endothelial growth factor (VEGF), produced by feline infectious peritonitis (FIP) virus-infected monocytes and macrophages, induces vascular permeability and effusion in cats with FIP","volume":"158","author":"Takano","year":"2011","journal-title":"Virus Res."},{"key":"ref_32","first-page":"729","article-title":"Meningoencephalitis and panophthalmitis in feline infectious peritonitis","volume":"160","author":"Slauson","year":"1972","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1939-1676.1998.tb02144.x","article-title":"Diagnostic features of clinical neurologic feline infectious peritonitis","volume":"12","author":"Foley","year":"1998","journal-title":"J. Vet. Intern. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0165-2427(95)05459-J","article-title":"Immunohistological demonstration of feline infectious peritonitis virus antigen in paraffin-embedded tissues using feline ascites or murine monoclonal antibodies","volume":"49","author":"Tammer","year":"1995","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1177\/0300985813505879","article-title":"When tissue antigens and antibodies get along: Revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique","volume":"51","author":"Miller","year":"2014","journal-title":"Vet. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1007\/s00705-020-04737-w","article-title":"Detection of SARS-CoV-2 by RNAscope(I) in situ hybridization and immunohistochemistry techniques","volume":"165","author":"Carossino","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3125","DOI":"10.1007\/s00705-016-3014-5","article-title":"Detection of equine arteritis virus by two chromogenic RNA in situ hybridization assays (conventional and RNAscoI(R))) and assessment of their performance in tissues from aborted equine fetuses","volume":"161","author":"Carossino","year":"2016","journal-title":"Arch. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Denes, L., Horvath, D.G., Duran, O., Ratkhjen, P.H., Kraft, C., Acs, B., Szasz, A.M., Rumenapf, T., Papp, M., and Ladinig, A. (2021). In Situ Hybridization of PRRSV-1 Combined with Digital Image Analysis in Lung Tissues of Pigs Challenged with PRRSV-1. Vet. Sci., 8.","DOI":"10.3390\/vetsci8100235"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1080\/22221751.2020.1833687","article-title":"SARS-CoV-2 infection, disease and transmission in domestic cats","volume":"9","author":"Gaudreault","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1177\/0300985815594852","article-title":"Localization of Bovine Papillomavirus Nucleic Acid in Equine Sarcoids","volume":"53","author":"Gaynor","year":"2016","journal-title":"Vet. Pathol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lumley, S., Hunter, L., Emery, K., Hewson, R., Fooks, A.R., Horton, D.L., and Johnson, N. (2021). Detection of Rift Valley Fever Virus RNA in Formalin-Fixed Mosquitoes by In Situ Hybridization (RNAIpe((R))). Viruses, 13.","DOI":"10.3390\/v13061079"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"491-e147","DOI":"10.1111\/vde.12787","article-title":"Quantitative real time polymerase chain reaction (qRT-PCR) and RNAscope in situ hybridization (RNA-ISH) as effective tools to diagnose feline herpesvirus-1-associated dermatitis","volume":"30","author":"Mazzei","year":"2019","journal-title":"Vet. Dermatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1177\/0300985818798085","article-title":"In Situ Hybridization for Localization of Ovine Herpesvirus 2, the Agent of Sheep-Associated Malignant Catarrhal Fever, in Formalin-Fixed Tissues","volume":"56","author":"Pesavento","year":"2019","journal-title":"Vet. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/PAI.0000000000000568","article-title":"RNA In Situ Hybridization for Epstein-Barr Virus and Cytomegalovirus: Comparison With In Situ Hybridization and Immunohistochemistry","volume":"27","author":"Roe","year":"2019","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1177\/1040638720915449","article-title":"In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma","volume":"32","author":"Szilasi","year":"2020","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0165-2427(98)00158-5","article-title":"Cellular composition, coronavirus antigen expression and production of specific antibodies in lesions in feline infectious peritonitis","volume":"65","author":"Kipar","year":"1998","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9975(98)80067-4","article-title":"Fatal enteritis associated with coronavirus infection in cats","volume":"119","author":"Kipar","year":"1998","journal-title":"J. Comp. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1080\/22221751.2021.1902753","article-title":"Experimental re-infected cats do not transmit SARS-CoV-2","volume":"10","author":"Gaudreault","year":"2021","journal-title":"Emerg. Microbes Infect."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/8\/883\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:04:52Z","timestamp":1760141092000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/8\/883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,5]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["pathogens11080883"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11080883","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,5]]}}}