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Babesiosis, the disease caused by Babesia infection, has been considered a potential threat to ruminant production due to the grave and enormous impact it brings. About 125 million ruminants are at risk of babesiosis in Southeast Asia (SEA), a region composed of 11 countries. In recent decades, molecular-based diagnostic platforms, such as polymerase chain reaction (PCR) assays, have been a reliable and broadly employed tool in Babesia detection. In this article, the authors compiled and summarized the molecular studies conducted on ruminant babesiosis and mapped the species, including B. bovis, B. bigemina, B. ovata, Babesia sp. Mymensingh, Babesia sp. Hue, and B. ovis, and determined the host diversity of ruminant Babesia in SEA.<\/jats:p>","DOI":"10.3390\/pathogens11080915","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T03:33:20Z","timestamp":1660534400000},"page":"915","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Molecular Reports of Ruminant Babesia in Southeast Asia"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2411-8401","authenticated-orcid":false,"given":"Eloiza May","family":"Galon","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqra","family":"Zafar","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwei","family":"Ji","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Li","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuowei","family":"Ma","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2780-110X","authenticated-orcid":false,"given":"Xuenan","family":"Xuan","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2006.01.035","article-title":"Babesia\u2014A historical overview","volume":"138","author":"Uilenberg","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1016\/j.meegid.2012.07.004","article-title":"Babesia: A world emerging","volume":"12","author":"Schnittger","year":"2012","journal-title":"Infect. Genet. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Abubakar, M.K., and Perera, P. (2019). Economic and health impact of the ticks in production animals. Ticks and Tick-Borne Pathogens, IntechOpen.","DOI":"10.5772\/intechopen.82510"},{"key":"ref_4","unstructured":"Leinbach, T., and Frederick, W. (2021, October 01). Southeast Asia. Encycl. Br., Available online: https:\/\/www.britannica.com\/place\/Southeast-Asia."},{"key":"ref_5","unstructured":"OECD (2017). Food and Agriculture Organization of the United Nations. OECD-FAO Agricultural Outlook 2017\u20132026, OECD."},{"key":"ref_6","first-page":"44","article-title":"Livestock production-current status in South and South-east Asia, future directions and priority areas for research","volume":"Volume 59","author":"Perera","year":"2014","journal-title":"Animal Production and Health Newsletter"},{"key":"ref_7","unstructured":"Food and Agriculture Organization (2021). Food and Agriculture Organization. Food and agriculture corporate statistical database 2019. FAOSTAT Database, Food and Agriculture Organization. Available online: http:\/\/faostat.fao.org\/."},{"key":"ref_8","unstructured":"Lee, T., and Hansen, J. (2021, October 22). Southeast Asia\u2019s growing meat demand and its implications for feedstuffs imports, Available online: https:\/\/www.ers.usda.gov\/amber-waves\/2019\/april\/southeast-asia-s-growing-meat-demand-and-its-implications-for-feedstuffs-imports\/."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40813-020-00159-x","article-title":"Major swine viral diseases: An Asian perspective after the African swine fever introduction","volume":"6","author":"Kedkovid","year":"2020","journal-title":"Porc. Health Manag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1079\/WPS200570","article-title":"Impact of avian influenza outbreaks in the poultry sectors of five South East Asian countries (Cambodia, Indonesia, Lao PDR, Thailand, Viet Nam) outbreak costs, responses and potential long term control","volume":"61","author":"Rushton","year":"2005","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_11","unstructured":"Park, C.-Y., Kumar, U., and San Andres, E.A. (2013). Food Security in Asia and the Pacific, Asian Development Bank."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Tan, L.P., Hamdan, R.H., Hassan, B.N.H., Reduan, M.F.H., Okene, I.A.-A., Loong, S.K., Khoo, J.J., Samsuddin, A.S., and Lee, S.H. (2021). Rhipicephalus tick: A contextual review for Southeast Asia. Pathogens, 10.","DOI":"10.3390\/pathogens10070821"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.pt.2011.12.002","article-title":"Is endemic stability of tick-borne disease in cattle a useful concept?","volume":"28","author":"Jonsson","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"175","DOI":"10.34101\/actaagrar\/1\/9101","article-title":"Perspectives of the livestock sector in the Philippines: A review","volume":"1","author":"Ortega","year":"2021","journal-title":"Acta Agrar. Debr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"968","DOI":"10.5713\/ajas.18.0201","article-title":"Current situation and future prospects for beef production in Thailand \u2014A review","volume":"31","author":"Bunmee","year":"2018","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_16","unstructured":"Hostiou, N., Duy, K.P., Cesaro, J.-D., Thanh, H.L.T., Duteurtre, G., Tien, D.N., Bonnet, P., and Cournut, S. (2016). The Transition of Animal Farming in Vietnam: From Semi-Subsistence to Commercial Systems, Centre de Coop\u00e9ration Internationale en Recherche Agronomique pour le D\u00e9veloppement (CIRAD). Available online: https:\/\/agritrop.cirad.fr\/583049\/1\/P31.pdf."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5149","DOI":"10.19082\/5149","article-title":"Molecular epidemiology of infectious diseases","volume":"9","author":"Eybpoosh","year":"2017","journal-title":"Electron. Physician"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.2174\/092986712799828355","article-title":"Current advances in detection and treatment of babesiosis","volume":"19","author":"Mosqueda","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Alvarez, J.A., Rojas, C., and Figueroa, J.V. (2019). Diagnostic tools for the identification of Babesia sp. in persistently infected cattle. Pathogens, 8.","DOI":"10.3390\/pathogens8030143"},{"key":"ref_20","first-page":"48","article-title":"A preliminary study on application of polymerase chain reaction in diagnosis of haemosporidiosis in cattle","volume":"6","author":"Hau","year":"1999","journal-title":"Vet. Sci. Tech."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1196\/annals.1373.076","article-title":"Molecular sequence evidence for the reclassification of some Babesia species","volume":"1081","author":"Allsopp","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.pt.2019.01.007","article-title":"Babesia life cycle\u2014When phylogeny meets biology","volume":"35","author":"Jalovecka","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-6851(99)00167-X","article-title":"A unique Babesia bovis spherical body protein is conserved among geographic isolates and localizes to the infected erythrocyte membrane","volume":"105","author":"Ruef","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.pt.2010.06.012","article-title":"Recent insights into alteration of red blood cells by Babesia bovis: Moovin\u2019 forward","volume":"26","author":"Gohil","year":"2010","journal-title":"Trends Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Allred, D. (2019). Variable and variant protein multigene families in Babesia bovis persistence. Pathogens, 8.","DOI":"10.3390\/pathogens8020076"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"104161","DOI":"10.1016\/j.meegid.2019.104161","article-title":"Use of molecular markers can help to understand the genetic diversity of Babesia bovis","volume":"79","author":"Simas","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0304-4017(93)90008-B","article-title":"Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood","volume":"50","author":"Figueroa","year":"1993","journal-title":"Vet. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.meegid.2017.08.009","article-title":"Low levels of genetic diversity associated with evidence of negative selection on the Babesia bovis apical membrane antigen 1 from parasite opulations in Thailand","volume":"54","author":"Rittipornlertrak","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1292\/jvms.12-0329","article-title":"A PCR based survey of Babesia ovata in cattle from various Asian, African and South American countries","volume":"75","author":"Yoshinari","year":"2013","journal-title":"J. Vet. Med. Sci."},{"key":"ref_30","first-page":"2233","article-title":"Host range and geographical distribution of Babesia sp. Mymensingh","volume":"67","author":"Sivakumar","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetpar.2012.01.008","article-title":"Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay","volume":"187","author":"Sivakumar","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vetpar.2009.12.013","article-title":"Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection","volume":"169","author":"AbouLaila","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.vetpar.2011.01.041","article-title":"Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand","volume":"178","author":"Terkawi","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1007\/s00436-012-2960-4","article-title":"Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand","volume":"111","author":"Cao","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1196\/annals.1428.038","article-title":"Improved molecular tools for detection of Babesia bigemina","volume":"1149","author":"Petrigh","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0166-6851(92)90097-4","article-title":"Ribosomal DNA sequence comparison of Babesia and Theileria","volume":"54","author":"Ellis","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.exppara.2012.11.005","article-title":"Development of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle","volume":"133","author":"Simuunza","year":"2013","journal-title":"Exp. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1128\/JCM.00220-12","article-title":"PCR detection of Babesia ovata from cattle reared in Japan and clinical significance of coinfection with Theileria orientalis","volume":"50","author":"Sivakumar","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e00895-18","DOI":"10.1128\/JCM.00895-18","article-title":"Genetic analysis of Babesia isolates from cattle with clinical babesiosis in Sri Lanka","volume":"56","author":"Sivakumar","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.vetpar.2005.05.057","article-title":"Development of a polymerase chain reaction method for diagnosis of Babesia ovis infection in sheep and goats","volume":"133","author":"Altay","year":"2005","journal-title":"Vet. Parasitol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1292\/jvms.13-0221","article-title":"PCR detection and genetic diversity of bovine hemoprotozoan parasites in Vietnam","volume":"75","author":"Sivakumar","year":"2013","journal-title":"J. Vet. Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1292\/jvms.16-0099","article-title":"Epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in Vietnam","volume":"78","author":"Weerasooriya","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1292\/jvms.17-0549","article-title":"Serological and molecular surveys of Babesia bovis and Babesia bigemina among native cattle and cattle imported from Thailand in Hue, Vietnam","volume":"80","author":"Sivakumar","year":"2018","journal-title":"J. Vet. Med. Sci."},{"key":"ref_44","first-page":"406","article-title":"Molecular and seroepidemiological survey of Babesia bovis and Babesia bigemina infections in cattle and water buffaloes in the central region of Vietnam","volume":"31","author":"Li","year":"2014","journal-title":"Trop. Biomed."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.meegid.2014.12.035","article-title":"Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes","volume":"30","author":"Yokoyama","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.vetpar.2015.05.019","article-title":"Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon island, the Philippines","volume":"212","author":"Ochirkhuu","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetpar.2013.02.013","article-title":"Molecular survey of bovine vector-borne pathogens in Cebu, Philippines","volume":"196","author":"Sivakumar","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1292\/jvms.12-0425","article-title":"Epidemiological survey of Babesia bovis and Babesia bigemina infections of cattle in Philippines","volume":"75","author":"Yu","year":"2013","journal-title":"J. Vet. Med. Sci."},{"key":"ref_49","first-page":"309","article-title":"Prevalence of babesiosis (Babesia bovis and Babesia bigemina) in cattle and water buffalo in Nueva Ecija, Philippines using nested polymerase chain reaction","volume":"63","author":"Herrera","year":"2017","journal-title":"Ann. Parasitol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1292\/jvms.19-0636","article-title":"Molecular survey of tick-borne pathogens infecting backyard cattle and water buffaloes in Quezon province, Philippines","volume":"82","author":"Galon","year":"2020","journal-title":"J. Vet. Med. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Prado, I.C.B., Capuno, L.X.B., Collera, P.D.L.P., Cabralda, A.P.D., De Ramos, K.A.S., Bernardo, J.M.G., Divina, B.P., Masatani, T., Tanaka, T., and Galay, R.L. (2022). Molecular detection and characterization of Babesia and Theileria in cattle and water buffaloes from southern Luzon, Philippines. Microorganisms, 10.","DOI":"10.3390\/microorganisms10040678"},{"key":"ref_52","first-page":"188","article-title":"Molecular evidence of Babesia spp. in cattle in the Philippines","volume":"14","author":"Foronda","year":"2010","journal-title":"Online J. Vet. Res."},{"key":"ref_53","first-page":"10","article-title":"Prevalence and risk factors of Babesia infections in cattle trespassing natural forest areas in Salakpra wildlife sanctuary, Kanchanaburi province","volume":"37","author":"Simking","year":"2014","journal-title":"J. Trop. Med. Parasitol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.parint.2015.10.005","article-title":"Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand","volume":"65","author":"Jirapattharasate","year":"2016","journal-title":"Parasitol. Internat."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1007\/s00436-016-5345-2","article-title":"Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand","volume":"116","author":"Jirapattharasate","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref_56","first-page":"32","article-title":"Detection of blood parasites infection in native cattle in Chiang Mai under the participatory one health disease and detection project","volume":"35","author":"Rittisut","year":"2018","journal-title":"J. Agric. Res. Ext."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.vetpar.2013.07.015","article-title":"The molecular prevalence and MSA-2b gene-based genetic dversity of Babesia bovis in dairy cattle in Thailand","volume":"197","author":"Simking","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Sawitri, D.H., Wardhana, A.H., Ekawasti, F., and Dewi, D.A. (2021, January 21\u201322). Parasitological and molecular detection of babesiosis in cattle and buffalo in west and central Java. Proceedings of the 9th International Seminar on Tropical Animal Production (ISTAP 2021), Yogyakarta, Indonesia.","DOI":"10.2991\/absr.k.220207.028"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13071-017-2502-0","article-title":"Molecular and serological detection of bovine babesiosis in Indonesia","volume":"10","author":"Guswanto","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ttbdis.2015.10.010","article-title":"Molecular survey of Babesia infections in cattle from different areas of Myanmar","volume":"7","author":"Bawm","year":"2016","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"101653","DOI":"10.1016\/j.ttbdis.2021.101653","article-title":"The molecular prevalence, distribution and risk factors associated with Babesia bigemina infection in Peninsular Malaysia","volume":"12","author":"Sharma","year":"2021","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_62","first-page":"13","article-title":"Molecular prevalence and species co-Infection of bovine haemoparasites in Peninsular Malaysia","volume":"8","author":"Maizatul","year":"2017","journal-title":"Malays. J. Vet. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","article-title":"Babesiosis of cattle","volume":"129","author":"Bock","year":"2004","journal-title":"Parasitology"},{"key":"ref_64","unstructured":"McLeod, R., and Kristjanson, P. (1999). Final report of joint eSYS\/ILRI\/ACIAR Tick Cost project\u2014Economic impact of ticks and tick-borne diseases to livestock in Africa, Asia and Australia. Int. Livest. Res. Inst. Nairobi., 3."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Florin-Christensen, M., and Schnittger, L. (2018). Babesia in domestic ruminants. Parasitic Protozoa of Farm Animals and Pets, Springer International Publishing.","DOI":"10.1007\/978-3-319-70132-5"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1292\/jvms.57.671","article-title":"Morphological, serological and antigenic characteristics, and protein profile of newly isolated Japanese bovine Babesia parasite with particular reference to those of B. ovata","volume":"57","author":"Ohta","year":"1995","journal-title":"J. Vet. Med. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2005.02.014","article-title":"Molecular phylogenetic studies on an unnamed bovine Babesia sp. based on small subunit ribosomal RNA gene sequences","volume":"133","author":"Luo","year":"2005","journal-title":"Vet. Parasitol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e231","DOI":"10.1111\/tbed.12745","article-title":"Molecular identification of tick-borne pathogens infecting cattle in Mymensingh district of Bangladesh reveals emerging species of Anaplasma and Babesia","volume":"65","author":"Roy","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1007\/s00436-022-07424-8","article-title":"The piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: Species compilation, molecular phylogeny, and evolutionary insights","volume":"121","author":"Schnittger","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1128\/CMR.16.4.622-636.2003","article-title":"Babesia divergens, a bovine blood parasite of veterinary and zoonotic importance","volume":"16","author":"Zintl","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.vetpar.2016.10.006","article-title":"Babesia ovata: Taxonomy, phylogeny and epidemiology","volume":"229","author":"Sivakumar","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1128\/JCM.00713-13","article-title":"Clinical bovine piroplasmosis caused by Babesia occultans in Italy","volume":"51","author":"Decaro","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1007\/s00436-021-07119-6","article-title":"First report of Babesia occultans in two symptomatic cows in Iran","volume":"120","author":"Noaman","year":"2021","journal-title":"Parasitol. Res."},{"key":"ref_74","unstructured":"Borghese, A., and Mazzi, M. (2005). Buffalo population and strategies in the world. Buffalo Production and Research, FAO regional office for Europe interregional cooperative research network on buffalo (ESCORENA)."},{"key":"ref_75","first-page":"55","article-title":"Babesiosis in an unusual case of Murrah buffalo with six functional teats","volume":"21","author":"Singla","year":"2002","journal-title":"Buffalo Bull."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/j.ttbdis.2018.04.012","article-title":"Mitigated clinical disease in water buffaloes experimentally infected with Babesia bovis","volume":"9","author":"Benitez","year":"2018","journal-title":"Ticks Tick-borne Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.3389\/fmicb.2017.01323","article-title":"A historical overview of research on Babesia orientalis, a protozoan parasite infecting water buffalo","volume":"8","author":"He","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.cyto.2009.02.006","article-title":"Comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses","volume":"46","author":"Mingala","year":"2009","journal-title":"Cytokine"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.ttbdis.2019.03.016","article-title":"First molecular detection and characterization of tick-borne pathogens in water buffaloes in Bohol, Philippines","volume":"10","author":"Galon","year":"2019","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3844\/ajavsp.2020.291.299","article-title":"Population, world production and quality of sheep and goat products","volume":"15","author":"Mazinani","year":"2020","journal-title":"Am. J. Anim. Vet. Sci."},{"key":"ref_81","unstructured":"Kumar, S., and Roy, M.M. (2013). Small ruminant\u2019s role in sustaining rural livelihoods in arid and semiarid regions and their potential for commercialization. New Paradigms in Livestock Production from Traditional to Commercial Farming and Beyond, Agrotech Publishing Academy."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1080\/00480169.2020.1719373","article-title":"The critical importance of planned small ruminant livestock health and production in addressing global challenges surrounding food production and poverty alleviation","volume":"68","author":"Sargison","year":"2020","journal-title":"N. Z. Vet. J."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1016\/0020-7519(95)00071-9","article-title":"Costs of major parasites to the Australian livestock industries","volume":"25","author":"Mcleod","year":"1995","journal-title":"Int. J. Parasitol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/vbz.2016.1955","article-title":"Guidelines for the detection of Babesia and Theileria parasites","volume":"17","author":"Lempereur","year":"2017","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0304-4017(97)00143-X","article-title":"Some epizootiological and clinical aspects of ovine babesiosis caused by Babesia ovis\u2014A review","volume":"74","author":"Yeruham","year":"1998","journal-title":"Vet. Parasitol."},{"key":"ref_86","first-page":"115","article-title":"Tick-borne diseases of sheep and goats and their related vectors in Iran","volume":"39","year":"1997","journal-title":"Parassitologia"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Stuen, S. (2020). Haemoparasites\u2014Challenging and wasting infections in small ruminants: A review. Animals, 10.","DOI":"10.3390\/ani10112179"},{"key":"ref_88","unstructured":"Smith, M.C., and Sherman, D.M. (2011). Goat Medicine, John Wiley and Sons."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01652176.1981.9693787","article-title":"Babesia crassa n. sp. (Sporozoa, Babesiidae) of domestic sheep in Iran","volume":"3","author":"Uilenberg","year":"1981","journal-title":"Vet. Q."},{"key":"ref_90","first-page":"99","article-title":"Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp.","volume":"39","author":"Friedhoff","year":"1997","journal-title":"Parassitologia"},{"key":"ref_91","unstructured":"National Research Center for Protozoan Diseases, Galon, E.M., Yba\u00f1ez, R.H., Macalanda, A.M., Liu, M., Tumwebaze, M.A., Byamukama, B., Ji, S., Zafar, I., and Li, J. (2022). First Molecular Confirmation of Caprine Piroplasma in the Philippine, Obihiro University of Agriculture and Veterinary Medicine. submitted."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"105957","DOI":"10.1016\/j.actatropica.2021.105957","article-title":"Development of a novel multiplex PCR assay for the detection and differentiation of Plasmodium caprae from Theileria luwenshuni and Babesia spp. in goats","volume":"220","author":"Tu","year":"2021","journal-title":"Acta Trop."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"774","DOI":"10.14202\/vetworld.2019.774-777","article-title":"Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines","volume":"12","author":"Arrabis","year":"2019","journal-title":"Vet. World"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Udonsom, R., Mahittikorn, A., and Jirapattharasate, C. (2022). Molecular detection and genetic diversity of tick-borne pathogens in goats from the southern part of Thailand. Pathogens, 11.","DOI":"10.3390\/pathogens11040477"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/8\/915\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:08:40Z","timestamp":1760141320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/8\/915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,14]]},"references-count":94,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["pathogens11080915"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11080915","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,14]]}}}