{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:21:23Z","timestamp":1793215283276,"version":"4.5.0"},"reference-count":63,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T00:00:00Z","timestamp":1666915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministero della Salute (Italy)","award":["IZS SI 03\/18 RC"],"award-info":[{"award-number":["IZS SI 03\/18 RC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Canine adenovirus type 1 (CAdV-1) is the causative agent of a systemic and potentially fatal viral disease of domestic and wild canids. In Italy, CAdV-1 infection has also been occasionally described in dogs, but information on the epidemiology and its genomic features is still limited. A study was conducted on 291 dogs suspected of infectious gastrointestinal disease. Samples collected from dogs in southern Italy between 2017 and 2020 were analyzed. Virological and histopathological assays were carried out. The presence of CAdVs and other canine viral enteropathogens was investigated, and sequence and phylogenetic analyses were performed. CAdV-1 was detected in six (2.1%) dead stray dogs alone or in mixed infections with other viruses. Gross lesions and histopathological findings referred to CAdV infection were observed, also involving the central nervous system tissues. All inoculated samples were successfully isolated. Sequence analysis evidenced divergences with the circulating strains previously described in Italy and a closer relation with older CAdV-1 strains collected from other countries, suggesting a genetic heterogeneity of CAdV-1 in Italy. The evidence of the circulation of CAdV-1 and its genomic features allows us to have more in-depth knowledge of the epidemiology and evolution of the CAdV-1 genomic variants.<\/jats:p>","DOI":"10.3390\/pathogens11111254","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T09:01:42Z","timestamp":1667120502000},"page":"1254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Study on the Canine Adenovirus Type 1 (CAdV-1) Infection in Domestic Dogs in Southern Italy"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1276-0579","authenticated-orcid":false,"given":"Francesco","family":"Mira","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3461-3353","authenticated-orcid":false,"given":"Roberto","family":"Puleio","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3323-6653","authenticated-orcid":false,"given":"Giorgia","family":"Schir\u00f2","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8535-9458","authenticated-orcid":false,"given":"Lucia","family":"Condorelli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6766-9126","authenticated-orcid":false,"given":"Santina","family":"Di Bella","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2804-4449","authenticated-orcid":false,"given":"Gabriele","family":"Chiaramonte","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-5321","authenticated-orcid":false,"given":"Giuseppa","family":"Purpari","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5768-5782","authenticated-orcid":false,"given":"Vincenza","family":"Cannella","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-6645","authenticated-orcid":false,"given":"Andrea","family":"Balboni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, Alma Mater Studiorum\u2014University of Bologna, Ozzano dell\u2019Emilia, 40064 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Randazzo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Antoci","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8902-6383","authenticated-orcid":false,"given":"Domenico","family":"Vicari","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Guercio","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, Via Gino Marinuzzi n. 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"ref_1","unstructured":"Rubarth, S. (1947). An Acute Virus-Disease with Liver Lesion in Dogs (Hepatitis Contagiosa Canis); a Pathologico-Anatomical and Etiological Investigation. Acta Pathol. Microbiol. Scand., Available online: https:\/\/www.ncbi.nlm.nih.gov\/nlmcatalog?cmd=PureSearch&term=0012136R%5Bnlmid%5D."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1111\/j.1751-0813.1949.tb13403.x","article-title":"Hepatitis Contagiosa Canis (Rubarth) in Australia","volume":"25","author":"Whittem","year":"1949","journal-title":"Aust. Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1136\/vr.62.38.359","article-title":"Viral Hepatitis of Dogs (Rubarth\u2019s Disease). I. Clinical and Pathological Observations on a Spontaneous Epidemic","volume":"62","author":"Parry","year":"1950","journal-title":"Vet. Rec."},{"key":"ref_4","unstructured":"Appel, M.J. (1987). Canine adenovirus type 1 (infectious canine hepatitis virus). Virus Infections of Carnivores, Elsevier Science."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2831","DOI":"10.1007\/s00705-014-2114-3","article-title":"Ratification Vote on Taxonomic Proposals to the International Committee on Taxonomy of Viruses (2014)","volume":"159","author":"Adams","year":"2014","journal-title":"Arch. Virol."},{"key":"ref_6","first-page":"234","article-title":"Evaluation of a Canine Adenovirus Type 2 Strain as a Replacement for Infectious Canine Hepatitis Vaccine","volume":"177","author":"Bass","year":"1980","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Bergmann, M., Freisl, M., Zablotski, Y., Speck, S., Truyen, U., and Hartmann, K. (2020). Antibody Response to Canine Adenovirus-2 Virus Vaccination in Healthy Adult Dogs. Viruses, 12.","DOI":"10.3390\/v12101198"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1354\/vp.44-4-536","article-title":"Canine Adenovirus Type 1 Infection of a Eurasian River Otter (Lutra lutra)","volume":"44","author":"Park","year":"2007","journal-title":"Vet. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.cvsm.2008.02.006","article-title":"Canine Adenoviruses and Herpesvirus","volume":"38","author":"Decaro","year":"2008","journal-title":"Vet. Clin. North. Am. Small Anim. Pract."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"642","DOI":"10.7589\/2017-10-245","article-title":"Infectious Canine Hepatitis in a Brown Bear (Ursus arctos horribilis) from Alaska, USA","volume":"54","author":"Knowles","year":"2018","journal-title":"J. Wildl. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s11259-019-09746-y","article-title":"Unique Genetic Features of Canine Adenovirus Type 1 (CAdV-1) Infecting Red Foxes (Vulpes vulpes) in Northern Norway and Arctic Foxes (Vulpes lagopus) in Svalbard","volume":"43","author":"Balboni","year":"2019","journal-title":"Vet. Res. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"935","DOI":"10.7589\/2018-12-295","article-title":"Multicentric Molecular and Pathologic Study on Canine Adenovirus Type 1 in Red Foxes (Vulpes vulpes) in Three European Countries","volume":"55","author":"Verin","year":"2019","journal-title":"J. Wildl. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1111\/tbed.14010","article-title":"Wolf (Canis lupus) as Canine Adenovirus Type 1 (CAdV-1) Sentinel for the Endangered Cantabrian Brown Bear (Ursus arctos arctos)","volume":"69","author":"Oleaga","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Balboni, A., Urbani, L., Delogu, M., Musto, C., Fontana, M.C., Merialdi, G., Lucifora, G., Terrusi, A., Dondi, F., and Battilani, M. (2021). Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus). Animals, 11.","DOI":"10.3390\/ani11082198"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1111\/tbed.13917","article-title":"Circulation of Diverse Protoparvoviruses in Wild Carnivores, Italy","volume":"68","author":"Ndiana","year":"2020","journal-title":"Transbound Emerg Dis"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.vetmic.2012.11.015","article-title":"Molecular Epidemiology of Canine Adenovirus Type 1 and Type 2 in Free-Ranging Red Foxes (Vulpes vulpes) in Italy","volume":"162","author":"Balboni","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.rvsc.2014.10.010","article-title":"Investigation of the Presence of Canine Adenovirus (CAdV) in Owned Dogs in Northern Italy","volume":"97","author":"Balboni","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"737","DOI":"10.7589\/2018-08-206","article-title":"Genetic Characterization of Canine Adenovirus Type 1 Detected by Real-Time Polymerase Chain Reaction in an Oral Sample of an Italian Wolf (Canis lupus)","volume":"55","author":"Balboni","year":"2019","journal-title":"J. Wildl. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.vetmic.2018.05.025","article-title":"Sequential Circulation of Canine Adenoviruses 1 and 2 in Captive Wild Carnivores, France","volume":"221","author":"Dowgier","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1111\/tbed.13013","article-title":"Canine Adenovirus Type 1 (CAdV-1) in Free-Ranging European Brown Bear (Ursus arctos arctos): A Threat for Cantabrian Population?","volume":"65","author":"Royo","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1292\/jvms.55.699","article-title":"Dual Infection with Canine Distemper Virus and Infectious Canine Hepatitis Virus (Canine Adenovirus Type 1) in a Dog","volume":"55","author":"Kobayashi","year":"1993","journal-title":"J. Vet. Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1024\/0036-7281.a000015","article-title":"Hepatitis contagiosa canis Infektion bei 4 Hunden in der Schweiz","volume":"152","author":"Decaro","year":"2010","journal-title":"Schweiz. Arch. Tierheilk."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1016\/j.bjm.2018.02.006","article-title":"Identification of Enteric Viruses Circulating in a Dog Population with Low Vaccine Coverage","volume":"49","author":"Alves","year":"2018","journal-title":"Braz. J. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13477","DOI":"10.1038\/s41598-018-31540-0","article-title":"Canine Morbillivirus (Canine Distemper Virus) with Concomitant Canine Adenovirus, Canine Parvovirus-2, and Neospora Caninum in Puppies: A Retrospective Immunohistochemical Study","volume":"8","author":"Headley","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hornsey, S.J., Philibert, H., Godson, D.L., and Snead, E.C.R. (2019). Canine Adenovirus Type 1 Causing Neurological Signs in a 5-Week-Old Puppy. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2173-5"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1046\/j.1439-0450.2001.00466.x","article-title":"Severe Enteric Disease in an Animal Shelter Associated with Dual Infections by Canine Adenovirus Type 1 and Canine Coronavirus","volume":"48","author":"Pratelli","year":"2001","journal-title":"J. Vet. Med. B"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.rvsc.2006.11.009","article-title":"Infectious Canine Hepatitis: An \u201cOld\u201d Disease Reemerging in Italy","volume":"83","author":"Decaro","year":"2007","journal-title":"Res. Vet. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.tvjl.2017.05.011","article-title":"Novel Sequence Variants of Viral Hexon and Fibre Genes in Two Dogs with Canine Adenovirus Type 1-Associated Disease","volume":"223","author":"Balboni","year":"2017","journal-title":"Vet. J."},{"key":"ref_29","first-page":"57","article-title":"Canine Adenovirus Type 1 and Pasteurella Pneumotropica Co-infection in a Puppy","volume":"52","author":"Pintore","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"80","DOI":"10.2478\/acve-2018-0007","article-title":"Investigation on Circulation in Dogs from Sicily (Italy) by Biomolecular Assay","volume":"68","author":"Purpari","year":"2018","journal-title":"Acta Vet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1023\/A:1006417203856","article-title":"Detection and Differentiation of CAV-1 and CAV-2 by Polymerase Chain Reaction","volume":"25","author":"Hu","year":"2001","journal-title":"Vet. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s11262-008-0314-1","article-title":"Molecular Characterization of Canine Parvovirus-2 Variants Circulating in Tunisia","volume":"38","author":"Touihri","year":"2009","journal-title":"Virus Genes"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1006\/viro.1993.1217","article-title":"Dolphin and Porpoise Morbilliviruses Are Genetically Distinct from Phocine Distemper Virus","volume":"193","author":"Barrett","year":"1993","journal-title":"Virology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1006\/mcpr.2001.0364","article-title":"Variation of the Sequence in the Gene Encoding for Transmembrane Protein M of Canine Coronavirus (CCV)","volume":"15","author":"Pratelli","year":"2001","journal-title":"Mol. Cell. Probes"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1002\/jmv.21228","article-title":"Enhancement of Detection and Quantification of Rotavirus in Stool Using a Modified Real-Time RT-PCR Assay","volume":"80","author":"Freeman","year":"2008","journal-title":"J. Med. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1111\/tbed.13379","article-title":"Nearly Full-length Genome Characterization of Canine Parvovirus Strains Circulating in Nigeria","volume":"67","author":"Ogbu","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1128\/JCM.42.4.1797-1799.2004","article-title":"Two Genotypes of Canine Coronavirus Simultaneously Detected in the Fecal Samples of Dogs with Diarrhea","volume":"42","author":"Pratelli","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.virusres.2008.12.009","article-title":"Sequence Analysis of Divergent Canine Coronavirus Strains Present in a UK Dog Population","volume":"141","author":"Erles","year":"2009","journal-title":"Virus Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"534","DOI":"10.3389\/fvets.2020.00534","article-title":"Novel Genotype Definition and the First Epidemiological Investigation of Canine Adenovirus Type 2 in Dogs in Central China","volume":"7","author":"Ji","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_40","first-page":"95","article-title":"Bioedit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program For Windows 95\/98\/ Nt","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/10665270050081478","article-title":"A Greedy Algorithm for Aligning DNA Sequences","volume":"7","author":"Zhang","year":"2000","journal-title":"J. Comput. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e00225-17","DOI":"10.1128\/genomeA.00225-17","article-title":"Genome Sequence of Canine Adenovirus Type 1 Isolated from a Wolf (Canis lupus) in Southern Italy","volume":"5","author":"Pizzurro","year":"2017","journal-title":"Genome Announc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/6207297","article-title":"Lifestyle as Risk Factor for Infectious Causes of Death in Young Dogs: A Retrospective Study in Southern Italy (2015\u20132017)","volume":"2020","author":"Cardillo","year":"2020","journal-title":"Vet. Med. Int."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Piegari, G., Cardillo, L., Alfano, F., Vangone, L., Iovane, V., and Fusco, G. (2020). Pathological, Bacteriological and Virological Findings in Sudden and Unexpected Deaths in Young Dogs. Animals, 10.","DOI":"10.3390\/ani10071134"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.vaccine.2006.10.020","article-title":"Occurrence of Severe Gastroenteritis in Pups after Canine Parvovirus Vaccine Administration: A Clinical and Laboratory Diagnostic Dilemma","volume":"25","author":"Decaro","year":"2007","journal-title":"Vaccine"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.vetmic.2016.09.006","article-title":"Enteropathogen Infections in Canine Puppies: (Co-)Occurrence, Clinical Relevance and Risk Factors","volume":"195","author":"Duijvestijn","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.vetmic.2017.04.007","article-title":"A Molecular Survey for Selected Viral Enteropathogens Revealed a Limited Role of Canine Circovirus in the Development of Canine Acute Gastroenteritis","volume":"204","author":"Dowgier","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1177\/104063870501700111","article-title":"Diagnosis of Infectious Canine Hepatitis Virus (CAV-1) Infection in Puppies with Encephalopathy","volume":"17","author":"Caudell","year":"2005","journal-title":"J. Vet. Diagn. Invest."},{"key":"ref_50","unstructured":"Sykes, J.E., and Greene, C.E. (2013). Infectious Diseases of the Dog and Cat, Elsevier Health Sciences. [4th ed.]."},{"key":"ref_51","first-page":"51","article-title":"Studies on Infectious Canine Hepatitis I: Histopathological Studies on Experimental Cases","volume":"5","author":"Fujimoto","year":"1957","journal-title":"Jpn. J. Vet. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jviromet.2015.05.009","article-title":"Development of a SYBR Green Real-Time PCR Assay with Melting Curve Analysis for Simultaneous Detection and Differentiation of Canine Adenovirus Type 1 and Type 2","volume":"222","author":"Balboni","year":"2015","journal-title":"J. Virol. Methods"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/tbed.14474","article-title":"Co-Circulation of Five Species of Dog Parvoviruses and Canine Adenovirus Type 1 among Gray Wolves (Canis lupus) in Northern Canada","volume":"69","author":"Canuti","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1439-0442.1956.tb00118.x","article-title":"Studies on Infectious Hepatitis in the Dog (I)","volume":"3","author":"Ochi","year":"2010","journal-title":"Zentralbl. Veterin\u00e4rmed."},{"key":"ref_55","first-page":"225","article-title":"Update on Canine Distemper Virus (CDV) Strains of Arctic-like Lineage Detected in Dogs in Italy","volume":"54","author":"Mira","year":"2018","journal-title":"Vet. Ital."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1111\/tbed.13283","article-title":"Spreading of Canine Parvovirus Type 2c Mutants of Asian Origin in Southern Italy","volume":"66","author":"Mira","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/tbed.12747","article-title":"Introduction of Asian Canine Parvovirus in Europe through Dog Importation","volume":"65","author":"Mira","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1136\/vr.103559","article-title":"Infectious canine hepatitis in red foxes (Vulpes vulpes) in wildlife rescue centres in the UK","volume":"178","author":"Walker","year":"2016","journal-title":"Vet. Rec."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"284","DOI":"10.21825\/vdt.v89i5.16956","article-title":"Infectious canine hepatitis, not only in the textbooks: A brief review and three case reports","volume":"89","author":"Chiers","year":"2020","journal-title":"Vlaams. Diergeneeskd. Tijdschr."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"243","DOI":"10.5326\/JAAHA-MS-6741","article-title":"2017 AAHA Canine Vaccination Guidelines","volume":"53","author":"Ford","year":"2017","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_61","first-page":"E1","article-title":"Vaccination Guidelines Group (VGG) of the World Small Animal Veterinary Association (WSAVA). WSAVA Guidelines for the vaccination of dogs and cats","volume":"57","author":"Day","year":"2016","journal-title":"J. Small Anim. Pract."},{"key":"ref_62","unstructured":"Dall\u2019Ara, P. (2020). Vaccini e vaccinazioni core del cane. Vaccini e Vaccinazioni degli Animali da Compagnia, EDRA."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.meegid.2016.10.020","article-title":"Lethal Distemper in Badgers (Meles meles) Following Epidemic in Dogs and Wolves","volume":"46","author":"Zaccaria","year":"2016","journal-title":"Infect. Genet. 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