{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:11:10Z","timestamp":1786493470448,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T00:00:00Z","timestamp":1668816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006345","name":"Nicolaus Copernicus University","doi-asserted-by":"publisher","award":["PDB WF 839"],"award-info":[{"award-number":["PDB WF 839"]}],"id":[{"id":"10.13039\/501100006345","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Viral infections, or their reactivations, are one of the most important groups of transplantation complications that can occur among recipients of both hematopoietic cells and solid organ transplants. They are the most commonly caused by cytomegalovirus (CMV). Currently, the use of whole blood or plasma samples is recommended for CMV viral load monitoring. The aim of the study was to assess and compare the level of CMV DNA, depending on the type of clinical material\u2014whole blood or plasma fraction derived from the same patient. The studies were carried out on 156 whole blood samples in which the presence of CMV genetic material was confirmed and the corresponding plasma samples from the same rounds of sampling. CMV DNA was not present in 59 (37.8%) of plasma samples compared to whole blood-positive counterparts. Of the samples positive in both types of clinical specimen, 77 (79.4%) had higher viral DNA levels in the whole blood samples. There were statistically significant differences in the detected CMV DNA load in the whole blood compared to plasma fraction counterparts (p &lt; 0.001). The detected CMV DNA value is usually higher in whole blood compared to plasma samples of the same patient. Due to the variability in CMV viral load depending on the clinical material used for a particular patient, one type of specimen should be always used consequently for CMV viremia monitoring.<\/jats:p>","DOI":"10.3390\/pathogens11111384","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T02:52:21Z","timestamp":1668999141000},"page":"1384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Whole Blood versus Plasma Samples\u2014How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia?"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1132-3505","authenticated-orcid":false,"given":"Mateusz","family":"Rzepka","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-094 Bydgoszcz, Poland"},{"name":"Department of Clinical Microbiology, Antoni Jurasz University Hospital No. 1, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2387-9778","authenticated-orcid":false,"given":"Dagmara","family":"Depka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-094 Bydgoszcz, Poland"},{"name":"Department of Clinical Microbiology, Antoni Jurasz University Hospital No. 1, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Gospodarek-Komkowska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-094 Bydgoszcz, Poland"},{"name":"Department of Clinical Microbiology, Antoni Jurasz University Hospital No. 1, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6787-1378","authenticated-orcid":false,"given":"Tomasz","family":"Bogiel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-094 Bydgoszcz, Poland"},{"name":"Department of Clinical Microbiology, Antoni Jurasz University Hospital No. 1, 85-094 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1159\/000322141","article-title":"Human Cytomegalovirus (HCMV)\u2014Revised*","volume":"37","author":"Schottstedt","year":"2010","journal-title":"Transfus. Med. Hemother."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/rmv.655","article-title":"Review of Cytomegalovirus Seroprevalence and Demographic Characteristics Associated with Infection","volume":"20","author":"Cannon","year":"2010","journal-title":"Rev. Med. Virol."},{"key":"ref_3","first-page":"23","article-title":"Prevention of Maternal Cytomegalovirus Infection: Current Status and Future Prospects","volume":"2","author":"Nyholm","year":"2010","journal-title":"Int. J. Womens Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1542\/peds.72.3.295","article-title":"Cytomegalovirus Infection of Breast Milk and Transmission in Infancy","volume":"72","author":"Dworsky","year":"1983","journal-title":"Pediatrics"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1056\/NEJM198005083021908","article-title":"Breast Milk and the Risk of Cytomegalovirus Infection","volume":"302","author":"Stagno","year":"1980","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cannon, M.J., and Davis, K.F. (2005). Washing Our Hands of the Congenital Cytomegalovirus Disease Epidemic. BMC Public Health, 5.","DOI":"10.1186\/1471-2458-5-70"},{"key":"ref_7","unstructured":"CDC (2022, June 07). About Cytomegalovirus and Congenital CMV Infection, Available online: https:\/\/www.cdc.gov\/cmv\/overview.html."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-3-540-77349-8_4","article-title":"Cytomegalovirus Cell Tropism","volume":"Volume 325","author":"Shenk","year":"2008","journal-title":"Human Cytomegalovirus"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/978-3-540-77349-8_23","article-title":"Manifestations of Human Cytomegalovirus Infection: Proposed Mechanisms of Acute and Chronic Disease","volume":"Volume 325","author":"Shenk","year":"2008","journal-title":"Human Cytomegalovirus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/s41579-021-00582-z","article-title":"Pathogenesis of Human Cytomegalovirus in the Immunocompromised Host","volume":"19","author":"Griffiths","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/978-3-540-77349-8_17","article-title":"Aspects of Human Cytomegalovirus Latency and Reactivation","volume":"Volume 325","author":"Shenk","year":"2008","journal-title":"Human Cytomegalovirus"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1099\/0022-1317-72-9-2059","article-title":"Monocytes Are a Major Site of Persistence of Human Cytomegalovirus in Peripheral Blood Mononuclear Cells","volume":"72","author":"Sissons","year":"1991","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1128\/JVI.73.6.4806-4812.1999","article-title":"Quantitative Analysis of Latent Human Cytomegalovirus","volume":"73","author":"Slobedman","year":"1999","journal-title":"J. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4054","DOI":"10.1128\/JVI.78.8.4054-4062.2004","article-title":"Impact of Human Cytomegalovirus Latent Infection on Myeloid Progenitor Cell Gene Expression","volume":"78","author":"Slobedman","year":"2004","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1056\/NEJMe1401567","article-title":"Taming the Transplantation Troll by Targeting Terminase","volume":"370","author":"Griffiths","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1097\/TP.0000000000002191","article-title":"The Transplantation Society International CMV Consensus Group the Third International Consensus Guidelines on the Management of Cytomegalovirus in Solid-Organ Transplantation","volume":"102","author":"Kotton","year":"2018","journal-title":"Transplantation"},{"key":"ref_17","first-page":"297","article-title":"Recommendations for the Diagnosis and Treatment of CMV Infections. Polish Society of Epidemiology and Infectious Diseases","volume":"70","author":"Niezgoda","year":"2016","journal-title":"Przegl. Epidemiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1182\/blood-2015-11-679639","article-title":"Early Cytomegalovirus Reactivation Remains Associated with Increased Transplant-Related Mortality in the Current Era: A CIBMTR Analysis","volume":"127","author":"Teira","year":"2016","journal-title":"Blood"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.bbmt.2010.03.020","article-title":"Evidence for a Bidirectional Relationship between Cytomegalovirus Replication and Acute Graft-versus-Host Disease","volume":"16","author":"Cantoni","year":"2010","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1097\/TP.0000000000000160","article-title":"Cytomegalovirus Serology and Replication Remain Associated with Solid Organ Graft Rejection and Graft Loss in the Era of Prophylactic Treatment","volume":"98","author":"Stern","year":"2014","journal-title":"Transplantation"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e260","DOI":"10.1016\/S1473-3099(19)30107-0","article-title":"Guidelines for the Management of Cytomegalovirus Infection in Patients with Haematological Malignancies and after Stem Cell Transplantation from the 2017 European Conference on Infections in Leukaemia (ECIL 7)","volume":"19","author":"Ljungman","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/cid\/ciw668","article-title":"Definitions of Cytomegalovirus Infection and Disease in Transplant Patients for Use in Clinical Trials","volume":"64","author":"Ljungman","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.biologicals.2016.04.005","article-title":"Collaborative Study Group A Collaborative Study to Establish the 1st WHO International Standard for Human Cytomegalovirus for Nucleic Acid Amplification Technology","volume":"44","author":"Fryer","year":"2016","journal-title":"Biologicals"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4105","DOI":"10.1128\/JCM.40.11.4105-4113.2002","article-title":"Human Cytomegalovirus DNA in Plasma and Serum Specimens of Renal Transplant Recipients Is Highly Fragmented","volume":"40","author":"Boom","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1093\/infdis\/jix069","article-title":"Determination of the Biological Form of Human Cytomegalovirus DNA in the Plasma of Solid-Organ Transplant Recipients","volume":"215","author":"Tong","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chiopris, G., Veronese, P., Cusenza, F., Procaccianti, M., Perrone, S., Dacc\u00f2, V., Colombo, C., and Esposito, S. (2020). Congenital Cytomegalovirus Infection: Update on Diagnosis and Treatment. Microorganisms, 8.","DOI":"10.3390\/microorganisms8101516"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Waters, S., Lee, S., Lloyd, M., Irish, A., and Price, P. (2019). The Detection of CMV in Saliva Can Mark a Systemic Infection with CMV in Renal Transplant Recipients. IJMS, 20.","DOI":"10.3390\/ijms20205230"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1097\/TP.0b013e3181ff8719","article-title":"The Clinical Utility of Whole Blood Versus Plasma Cytomegalovirus Viral Load Assays for Monitoring Therapeutic Response","volume":"91","author":"Lisboa","year":"2011","journal-title":"Transplantation"},{"key":"ref_29","first-page":"116","article-title":"CMV Viral Load Measurements in Whole Blood and Plasma\u2014Which Is Best Following Renal Transplantation?","volume":"70","author":"Breuer","year":"2000","journal-title":"Transplantation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jcv.2006.01.002","article-title":"Whole Blood Real-Time Quantitative PCR for Cytomegalovirus Infection Follow-up in Transplant Recipients","volume":"36","author":"Garrigue","year":"2006","journal-title":"J. Clin. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1097\/00007890-200203270-00025","article-title":"The Clinical Use of Various Blood Compartments for Cytomegalovirus (CMV) DNA Quantitation in Transplant Recipients with CMV Disease","volume":"73","author":"Razonable","year":"2002","journal-title":"Transplantation"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1097\/PDM.0b013e3181599242","article-title":"Cytomegalovirus DNA Measurement in Blood and Plasma Using Roche LightCycler CMV Quantification Reagents","volume":"17","author":"Tang","year":"2008","journal-title":"Diagn. Mol. Pathol."},{"key":"ref_33","first-page":"186","article-title":"Comparison of Two Molecular Assays for Detection of Cytomegalovirus DNA in Whole Blood and Plasma Samples from Transplant Recipients","volume":"39","author":"Costa","year":"2016","journal-title":"New Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.jviromet.2008.08.012","article-title":"Detection of CMV DNA: Is EDTA Whole Blood Superior to EDTA Plasma?","volume":"154","author":"Koidl","year":"2008","journal-title":"J. Virol. Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1172\/JCI116635","article-title":"Circulating Endothelial Giant Cells Permissive for Human Cytomegalovirus (HCMV) Are Detected in Disseminated HCMV Infections with Organ Involvement","volume":"92","author":"Percivalle","year":"1993","journal-title":"J. Clin. Investig."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.diagmicrobio.2018.11.012","article-title":"Factors Influencing Cytomegalovirus DNA Load Measurements in Whole Blood and Plasma Specimens from Allogeneic Hematopoietic Stem Cell Transplant Recipients","volume":"94","author":"Vinuesa","year":"2019","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/JCM.03435-14","article-title":"Monitoring of Cytomegalovirus Viral Loads by Two Molecular Assays in Whole-Blood and Plasma Samples from Hematopoietic Stem Cell Transplant Recipients","volume":"53","author":"Babady","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1016\/j.bbmt.2018.03.005","article-title":"Cytomegalovirus and Epstein-Barr Virus DNA Kinetics in Whole Blood and Plasma of Allogeneic Hematopoietic Stem Cell Transplantation Recipients","volume":"24","author":"Lazzarotto","year":"2018","journal-title":"Biol. Blood Marrow Transplant."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1093\/cid\/ciw370","article-title":"Are We There Yet? Impact of the First International Standard for Cytomegalovirus DNA on the Harmonization of Results Reported on Plasma Samples","volume":"63","author":"Preiksaitis","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1128\/JCM.01499-07","article-title":"Prediction of Cytomegalovirus (CMV) Plasma Load from Evaluation of CMV Whole-Blood Load in Samples from Renal Transplant Recipients","volume":"46","author":"Garrigue","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1093\/cid\/cis212","article-title":"Interpreting Quantitative Cytomegalovirus DNA Testing: Understanding the Laboratory Perspective","volume":"54","author":"Kraft","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1128\/CMR.00015-13","article-title":"Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation","volume":"26","author":"Razonable","year":"2013","journal-title":"Clin. Microbiol. Rev."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/11\/1384\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:59Z","timestamp":1760145719000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/11\/11\/1384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,19]]},"references-count":42,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["pathogens11111384"],"URL":"https:\/\/doi.org\/10.3390\/pathogens11111384","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,19]]}}}