{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T02:18:52Z","timestamp":1787192332396,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860565"],"award-info":[{"award-number":["81860565"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["102"],"award-info":[{"award-number":["102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["YNWR-MY-2019-008"],"award-info":[{"award-number":["YNWR-MY-2019-008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Innovation Team of Natural Focal Diseases Epidemiology in the University of Yunnan Province","award":["81860565"],"award-info":[{"award-number":["81860565"]}]},{"name":"Science and Technology Innovation Team of Natural Focal Diseases Epidemiology in the University of Yunnan Province","award":["102"],"award-info":[{"award-number":["102"]}]},{"name":"Science and Technology Innovation Team of Natural Focal Diseases Epidemiology in the University of Yunnan Province","award":["YNWR-MY-2019-008"],"award-info":[{"award-number":["YNWR-MY-2019-008"]}]},{"name":"the project of \u201cTalent Support Program in Yunnan\u201d","award":["81860565"],"award-info":[{"award-number":["81860565"]}]},{"name":"the project of \u201cTalent Support Program in Yunnan\u201d","award":["102"],"award-info":[{"award-number":["102"]}]},{"name":"the project of \u201cTalent Support Program in Yunnan\u201d","award":["YNWR-MY-2019-008"],"award-info":[{"award-number":["YNWR-MY-2019-008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Small mammals can transmit and serve as a reservoir for Orientia tsutsugamushi (Ot) in nature by carrying infected mites. In Yunnan, one of China\u2019s main foci of scrub typhus, etiological evidence and genetic diversity for Ot is limited. A total of 2538 small mammals were captured seasonally from 2015 to 2016 in the three counties of Yunnan, and the spleen or liver tissue was examined for Ot based on 56 kDa nPCR. The overall prevalence of Ot was 1.77%, ranging from 0.26 to 9.09% across different species. The Gilliam strain was found in 35.6% (16\/45) of the wild small mammals, followed by the Karp 11.1% (5\/45) and TA763 (1\/45) strains, the last of which was discovered in western Yunnan for the first time. In Lianghe, Ot infection rates in wild small mammals were higher than in the other two counties. The infection rates of Eothenomys miletus with Ot were highest in the three dominant species. Ot infection rates in wild small mammals were higher in Lianghe (1200\u20131400 m) and Yulong (2800\u20133000 m). These findings could provide research clues for further confirmation of scrub typhus foci in western Yunnan or other similar natural environments.<\/jats:p>","DOI":"10.3390\/pathogens12010128","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T01:30:26Z","timestamp":1673573426000},"page":"128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Orientia tsutsugamushi Infection in Wild Small Mammals in Western Yunnan Province, China"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0716-1889","authenticated-orcid":false,"given":"Yun-Yan","family":"Luo","sequence":"first","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Nan","family":"He","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru-Dan","family":"Hong","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7662-4915","authenticated-orcid":false,"given":"Jun-Jie","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Qiong","family":"Ai","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Xiang","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Public Health, Dali University, Dali City 671000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jiang, J., and Richards, A.L. (2018). Scrub Typhus: No Longer Restricted to the Tsutsugamushi Triangle. Trop. Med. Infect. Dis., 3.","DOI":"10.3390\/tropicalmed3010011"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Xu, G., Walker, D.H., Jupiter, D., Melby, P.C., and Arcari, C.M. (2017). A review of the global epidemiology of scrub typhus. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0006062"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.3201\/eid2609.200918","article-title":"Molecular Description of a Novel Orientia Species Causing Scrub Typhus in Chile","volume":"26","author":"Abarca","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13071-015-0784-7","article-title":"Detection of Orientia sp. DNA in rodents from Asia, West Africa and Europe","volume":"8","author":"Cosson","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, W.-Y., Wang, L.-Y., Ding, F., Hu, W.-B., Magalhaes, R.J.S., Sun, H.-L., Liu, Y.-X., Liu, Q.-Y., Huang, L.-Y., and Clements, A.C.A. (2013). Scrub Typhus in Mainland China, 2006\u20132012: The Need for Targeted Public Health Interventions. PLoS Neglected Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002493"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"De, W., Jing, K., Huan, Z., Qiong, Z.H., Monagin, C., Min, Z.J., Ping, H., Wen, K.C., and Yan, L.J. (2015). Scrub Typhus, a Disease with Increasing Threat in Guangdong, China. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0113968"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/1748-5967.12486","article-title":"Bimonthly surveillance of wild rodents and chigger mites in urban parks in Daegu from 2018 to 2019","volume":"50","author":"Kim","year":"2020","journal-title":"Entomol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s10661-019-7208-7","article-title":"Distribution of Orientia tsutsugamushi in rodents and mites collected from Central India","volume":"191","author":"Akhunji","year":"2019","journal-title":"Environ. Monit. Assess."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s13071-019-3751-x","article-title":"Scrub typhus ecology: A systematic review of Orientia in vectors and hosts","volume":"12","author":"Elliott","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"616","DOI":"10.4269\/ajtmh.17-0070","article-title":"Molecular Epidemiology of an Orientia tsutsugamushi Gene Encoding a 56-kDa Type-Specific Antigen in Chiggers, Small Mammals, and Patients from the Southwest Region of Korea","volume":"98","author":"Park","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4269\/ajtmh.2003.69.519","article-title":"Occurrence of Orientia tsutsugamushi in Small Mammals from Thailand","volume":"69","author":"Coleman","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1089\/vbz.2015.1831","article-title":"Prevalence and Phylogenetic Analysis of Orientia tsutsugamushi in Small Mammals in Hanoi, Vietnam","volume":"16","author":"Hotta","year":"2016","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.meegid.2017.10.014","article-title":"Orientia tsutsugamushi infection in rodents in Anhui Province of China","volume":"56","author":"Latif","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.3201\/eid1907.120984","article-title":"Genetic Variants of Orientia tsutsugamushiin Domestic Rodents, Northern China","volume":"19","author":"Zhang","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1007\/s12013-013-9646-0","article-title":"Consistency of the Key Genotypes of Orientia tsutsugamushi in Scrub Typhus Patients, Rodents, and Chiggers from a New Endemic Focus of Northern China","volume":"67","author":"Liu","year":"2013","journal-title":"Cell Biochem. Biophys."},{"key":"ref_16","first-page":"255","article-title":"Investigation on scrub typhus in Yongshan county, Yunnan Province, China","volume":"34","author":"Ya","year":"2018","journal-title":"Chin. J. Zoonoses."},{"key":"ref_17","first-page":"32","article-title":"Investigation of several bacterial pathogens on the wild small mammals in Gongshan area, Yunnan province","volume":"25","author":"Li","year":"2018","journal-title":"Acta Parasitol. Med. Entomol. Sin."},{"key":"ref_18","first-page":"145","article-title":"Investigation of Orientia tsutsugamushi infection in rodents and human in Yunnan","volume":"31","author":"Ya","year":"2016","journal-title":"Dis. Surveil."},{"key":"ref_19","first-page":"29901","article-title":"Comparative Analysis of Small Mammal and Parasitic Flea Composition in Yulong and Jianchuan Plague Foci in Yunnan Province, China: A Cross-Sectional Study","volume":"37","author":"Yin","year":"2021","journal-title":"Biomed. J. Sci. Tech. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Olson, N.D., and Morrow, J.B. (2012). DNA extract characterization process for microbial detection methods development and validation. BMC Res. Notes, 5.","DOI":"10.1186\/1756-0500-5-668"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1093\/molbev\/mst012","article-title":"Building Phylogenetic Trees from Molecular Data with MEGA","volume":"30","author":"Hall","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wei, Y., Huang, Y., Li, X., Ma, Y., Tao, X., Wu, X., and Yang, Z. (2017). Climate variability, animal reservoir and transmission of scrub typhus in Southern China. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005447"},{"key":"ref_24","first-page":"196","article-title":"Investigation on rickettsiae natural infection in mice and vector arthropods in partial areas of Yunnan","volume":"23","author":"Ya","year":"2019","journal-title":"Chin. J. Dis. Control. Pre."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"20737","DOI":"10.1038\/srep20737","article-title":"Determination of Scrub typhus Suggests a New Epidemic Focus in the Anhui Province of China","volume":"6","author":"Cao","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_26","first-page":"165","article-title":"Flea species diversity, spatial distribution and the relations to human plague in Yunnan Southwest moutain farmlan","volume":"28","author":"Dong","year":"2021","journal-title":"Acta Parasitol. Med. Entomol. Sin."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41598-020-73690-0","article-title":"The relationship between fleas and small mammals in households of the Western Yunnan Province, China","volume":"10","author":"Yin","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s13071-015-1221-7","article-title":"Surveillance of potential hosts and vectors of scrub typhus in Taiwan","volume":"8","author":"Kuo","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_29","first-page":"19","article-title":"Epidemiology and Diversity of Rickettsiales Bacteria in Humans and Animals in Jiangsu and Jiangxi provinces, China","volume":"9","author":"Lu","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"559","DOI":"10.3347\/kjp.2020.58.5.559","article-title":"Epidemiological Characteristics of Rodents and Chiggers with Orientia tsutsugamushi in the Republic of Korea","volume":"58","author":"Park","year":"2020","journal-title":"Korean J. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.ijid.2021.02.069","article-title":"Descriptive epidemiology of rickettsial infections in Japan: Scrub typhus and Japanese spotted fever, 2007\u20132016","volume":"105","author":"Kinoshita","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Li, T., Yang, Z., Dong, Z., and Wang, M. (2014). Meteorological factors and risk of scrub typhus in Guangzhou, southern China, 2006\u20132012. BMC Infect. Dis., 14.","DOI":"10.1186\/1471-2334-14-139"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wu, Y.-C., Qian, Q., Magalhaes, R.J.S., Han, Z.-H., Hu, W.-B., Haque, U., Weppelmann, T.A., Wang, Y., Liu, Y.-X., and Li, X.-L. (2016). Spatiotemporal Dynamics of Scrub Typhus Transmission in Mainland China, 2006-2014. PLoS Neglected Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004875"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1128\/AEM.02796-10","article-title":"Genetic Typing, Based on the 56-Kilodalton Type-Specific Antigen Gene, of Orientia tsutsugamushi Strains Isolated from Chiggers Collected from Wild-Caught Rodents in Taiwan","volume":"77","author":"Lin","year":"2011","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/1\/128\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:04:29Z","timestamp":1760119469000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/1\/128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,12]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["pathogens12010128"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12010128","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,12]]}}}