{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:58:21Z","timestamp":1786985901787,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T00:00:00Z","timestamp":1674432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["22-15-00216"],"award-info":[{"award-number":["22-15-00216"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Diopside-based ceramic is a perspective biocompatible material with numerous potential applications in the field of bone prosthetics. Implantable devices and materials are often prone to colonization and biofilm formation by pathogens such as Staphylococcus aureus, which in the case of bone grafting leads to osteomyelitis, an infectious bone and bone marrow injury. To lower the risk of bacterial colonization, implanted materials can be impregnated with antimicrobials. In this work, we loaded the antibacterial enzyme lysostaphin on diopside powder and studied the antibacterial and antibiofilm properties of such material to probe the utility of this approach for diopside-based prosthetic materials. Methods: Diopside powder was synthesized by the solid-state method, lysostaphin was loaded on diopside by adsorption, the release of lysostaphin from diopside was monitored by ELISA, and antibacterial and anti-biofilm activity was assessed by standard microbiological procedures. Results and conclusions: Lysostaphin released from diopside powder showed high antibacterial activity against planktonic bacteria and effectively destroyed 24-h staphylococcal biofilms. Diopside-based materials possess a potential for the development of antibacterial bone grafting materials.<\/jats:p>","DOI":"10.3390\/pathogens12020177","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T05:46:11Z","timestamp":1674452771000},"page":"177","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin"],"prefix":"10.3390","volume":"12","author":[{"given":"Alina","family":"Kudinova","sequence":"first","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1436-9015","authenticated-orcid":false,"given":"Alexander","family":"Grishin","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"},{"name":"All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Grunina","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"},{"name":"All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Poponova","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna","family":"Bulygina","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"},{"name":"Center for Biomedical Engineering, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Gromova","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6370-8071","authenticated-orcid":false,"given":"Rajan","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Rudolfs Cimdins Riga Biomaterials Innovations and Development Centre of RTU, Institute of General Chemical Engineering, Faculty of Materials Science and Applied Chemistry, Riga Technical University, Pulka St 3, LV-1007 Riga, Latvia"},{"name":"Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Kipsala Street 6A, LV-1048 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fedor","family":"Senatov","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"},{"name":"Center for Biomedical Engineering, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Karyagina","sequence":"additional","affiliation":[{"name":"Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia"},{"name":"Center for Biomedical Engineering, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"},{"name":"Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1097\/BOT.0000000000000043","article-title":"Can We Trust Intraoperative Culture Results in Nonunions?","volume":"28","author":"Palmer","year":"2014","journal-title":"J. Orthop. Trauma"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e00932-19","DOI":"10.1128\/IAI.00932-19","article-title":"Staphylococcus aureus Osteomyelitis: Bone, Bugs, and Surgery","volume":"88","author":"Urish","year":"2020","journal-title":"Infect. Immun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1056\/NEJMra035415","article-title":"Treatment of Infections Associated with Surgical Implants","volume":"350","author":"Darouiche","year":"2004","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0002-9343(03)00231-6","article-title":"Outcomes of Osteomyelitis among Patients Treated with Outpatient Parenteral Antimicrobial Therapy","volume":"114","author":"Tice","year":"2003","journal-title":"Am. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"212S","DOI":"10.1097\/01.prs.0000222737.09322.77","article-title":"Diagnosis and Treatment of Diabetic Foot Infections","volume":"117","author":"Lipsky","year":"2006","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1002\/jor.24293","article-title":"2018 International Consensus Meeting on Musculoskeletal Infection: Research Priorities from the General Assembly Questions","volume":"37","author":"Schwarz","year":"2019","journal-title":"J. Orthop. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1097\/TA.0b013e31818c6a09","article-title":"Treatment of Long Bone Osteomyelitis With a Mechanically Stable Intramedullar Antibiotic Dispenser: Nineteen Consecutive Cases with a Minimum of 12 Months Follow-Up","volume":"65","author":"Sancineto","year":"2008","journal-title":"J. Trauma Inj. Infect. Crit. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1097\/SAP.0b013e3181cf9fb5","article-title":"The Treatment of Gustilo Grade IIIB Tibia Fractures with Application of Antibiotic Spacer, Flap, and Sequential Distraction Osteogenesis","volume":"64","author":"Hutson","year":"2010","journal-title":"Ann. Plast. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1007\/s00264-016-3142-2","article-title":"Testing of antibiotic releasing implant coatings to fight bacteria in combat-associated osteomyelitis\u2014An in-vitro study","volume":"40","author":"Back","year":"2016","journal-title":"Int. Orthop."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9070216","DOI":"10.1155\/2018\/9070216","article-title":"Management of Osteomyelitis-Induced Massive Tibial Bone Defect by Monolateral External Fixator Combined with Antibiotics-Impregnated Calcium Sulphate: A Retrospective Study","volume":"2018","author":"Qin","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fcimb.2020.00107","article-title":"Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus","volume":"10","author":"Guo","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"742","DOI":"10.3389\/fchem.2019.00742","article-title":"Small Molecule Anti-Biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation","volume":"7","author":"Qvortrup","year":"2019","journal-title":"Front. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.3390\/ph3041139","article-title":"Lysostaphin: A Staphylococcal Bacteriolysin with Potential Clinical Applications","volume":"3","author":"Bastos","year":"2010","journal-title":"Pharmaceuticals"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1128\/AAC.47.11.3407-3414.2003","article-title":"Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus Epidermidis Biofilms on Artificial Surfaces","volume":"47","author":"Wu","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3256","DOI":"10.1128\/AAC.49.8.3256-3263.2005","article-title":"Comparison of Four Methods for Determining Lysostaphin Susceptibility of Various Strains of Staphylococcus aureus","volume":"49","author":"Kusuma","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.mib.2016.05.006","article-title":"Cell Wall Hydrolases and Antibiotics: Exploiting Synergy to Create Efficacious New Antimicrobial Treatments","volume":"33","author":"Wittekind","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Windolf, C.D., L\u00f6gters, T., Scholz, M., Windolf, J., and Floh\u00e9, S. (2014). Lysostaphin-Coated Titan-Implants Preventing Localized Osteitis by Staphylococcus aureus in a Mouse Model. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115940"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"E4960","DOI":"10.1073\/pnas.1801013115","article-title":"Hydrogel Delivery of Lysostaphin Eliminates Orthopedic Implant Infection by Staphylococcus aureus and Supports Fracture Healing","volume":"115","author":"Johnson","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"eaaw1228","DOI":"10.1126\/sciadv.aaw1228","article-title":"Lysostaphin and BMP-2 Co-Delivery Reduces S. aureus Infection and Regenerates Critical-Sized Segmental Bone Defects","volume":"5","author":"Johnson","year":"2019","journal-title":"Sci. Adv."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1098\/rsfs.2012.0012","article-title":"A Review of Protein Adsorption on Bioceramics","volume":"2","author":"Wang","year":"2012","journal-title":"Interface Focus"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.bioactmat.2021.05.032","article-title":"Factors Influencing the Drug Release from Calcium Phosphate Cements","volume":"7","author":"Fosca","year":"2022","journal-title":"Bioact. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1016\/j.apt.2019.06.014","article-title":"Biomineralization, Antibacterial Activity and Mechanical Properties of Biowaste Derived Diopside Nanopowders","volume":"30","author":"Choudhary","year":"2019","journal-title":"Adv. Powder Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"032001","DOI":"10.1088\/1748-6041\/8\/3\/032001","article-title":"A Review of Bioactive Silicate Ceramics","volume":"8","author":"Wu","year":"2013","journal-title":"Biomed. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/jbm.b.30779","article-title":"Degradation, Bioactivity, and Cytocompatibility of Diopside, Akermanite, and Bredigite Ceramics","volume":"83B","author":"Wu","year":"2007","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.actbio.2009.12.022","article-title":"Porous Diopside (CaMgSi2O6) Scaffold: A Promising Bioactive Material for Bone Tissue Engineering","volume":"6","author":"Wu","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1016\/j.biopha.2009.01.007","article-title":"Proliferation and Gene Expression of Osteoblasts Cultured in DMEM Containing the Ionic Products of Dicalcium Silicate Coating","volume":"63","author":"Sun","year":"2009","journal-title":"Biomed. Pharmacother."},{"key":"ref_27","first-page":"613","article-title":"Two Variants of Recombinant Human Bone Morphogenetic Protein-2 (RhBMP-2) with Additional Protein Domains: Synthesis in an Escherichia Coli Heterologous Expression System","volume":"82","author":"Karyagina","year":"2017","journal-title":"Biochemistry"},{"key":"ref_28","first-page":"1277","article-title":"Hybrid Implants Based on Calcium-Magnesium Silicate Ceramics Diopside as a Carrier of Recombinant BMP-2 and Demineralized Bone Matrix as a Scaffold: Dynamics of Reparative Osteogenesis in a Mouse Craniotomy Model","volume":"87","author":"Karyagina","year":"2022","journal-title":"Biochemistry"},{"key":"ref_29","first-page":"502","article-title":"Staphylococcus Simulans Recombinant Lysostaphin: Production, Purification, and Determination of Antistaphylococcal Activity","volume":"81","author":"Boksha","year":"2016","journal-title":"Biochemistry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6519","DOI":"10.1007\/s00253-022-12173-w","article-title":"Influence of NaCl and PH on Lysostaphin Catalytic Activity, Cell Binding, and Bacteriolytic Activity","volume":"106","author":"Konstantinova","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Grishin, A.V., Lavrova, N.V., Lyashchuk, A.M., Strukova, N.V., Generalova, M.S., Ryazanova, A.V., Shestak, N.V., Boksha, I.S., Polyakov, N.B., and Galushkina, Z.M. (2019). The Influence of Dimerization on the Pharmacokinetics and Activity of an Antibacterial Enzyme Lysostaphin. Molecules, 24.","DOI":"10.3390\/molecules24101879"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Grishin, A.V., Shestak, N.V., Lavrova, N.V., Lyashchuk, A.M., Popova, L.I., Strukova, N.V., Generalova, M.S., Ryazanova, A.V., Polyakov, N.B., and Galushkina, Z.M. (2019). Fusion of Lysostaphin to an Albumin Binding Domain Prolongs Its Half-Life and Bactericidal Activity in the Systemic Circulation. Molecules, 24.","DOI":"10.3390\/molecules24162892"},{"key":"ref_33","unstructured":"R Core Team (2013). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: http:\/\/www.R-project.org\/."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"126157","DOI":"10.1016\/j.matchemphys.2022.126157","article-title":"Comparative Investigation on Antibacterial, Biological and Mechanical Behaviour of Monticellite and Diopside Derived from Biowaste for Bone Regeneration","volume":"286","author":"Venkatraman","year":"2022","journal-title":"Mater. Chem. Phys."},{"key":"ref_35","first-page":"e50854","article-title":"Establishing the minimal bactericidal concentration of an antimicrobial agent for planktonic cells (MBC-P) and biofilm cells (MBC-B)","volume":"83","author":"Mah","year":"2014","journal-title":"J. Vis. Exp."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.ceramint.2019.08.290","article-title":"Vancomycin Release Kinetics from Mg\u2013Ca Silicate Porous Microspheres Developed for Controlled Drug Delivery","volume":"46","author":"Zirak","year":"2020","journal-title":"Ceram Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.actbio.2009.09.025","article-title":"Porous Bioactive Diopside (CaMgSi2O6) Ceramic Microspheres for Drug Delivery","volume":"6","author":"Wu","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1128\/AAC.47.2.554-558.2003","article-title":"Improved Pharmacokinetics and Reduced Antibody Reactivity of Lysostaphin Conjugated to Polyethylene Glycol","volume":"47","author":"Walsh","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Abdelkader, K., Gerstmans, H., Saafan, A., Dishisha, T., and Briers, Y. (2019). The Preclinical and Clinical Progress of Bacteriophages and Their Lytic Enzymes: The Parts Are Easier than the Whole. Viruses, 11.","DOI":"10.3390\/v11020096"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ceramint.2021.08.298","article-title":"Fabrication, Drug Delivery Kinetics and Cell Viability Assay of PLGA-Coated Vancomycin-Loaded Silicate Porous Microspheres","volume":"48","author":"Zirak","year":"2022","journal-title":"Ceram. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3883","DOI":"10.2147\/IJN.S162262","article-title":"Nanoporous diopside modulates biocompatibility, degradability and osteogenesis of bioactive scaffolds of gliadin-based composites for new bone formation","volume":"13","author":"Ba","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2217\/fmb.12.97","article-title":"Bacteriophage Endolysins as Novel Antimicrobials","volume":"7","author":"Schmelcher","year":"2012","journal-title":"Future Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105844","DOI":"10.1016\/j.ijantimicag.2019.11.001","article-title":"Endolysins as Emerging Alternative Therapeutic Agents to Counter Drug-Resistant Infections","volume":"55","author":"Gondil","year":"2020","journal-title":"Int. J. Antimicrob. Agents"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/2\/177\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:14:07Z","timestamp":1760120047000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/2\/177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,23]]},"references-count":43,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["pathogens12020177"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12020177","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,23]]}}}