{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:12:25Z","timestamp":1792789945370,"version":"4.5.0"},"reference-count":91,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"E.H. PhD grants from AUF, USEK, and CNRS-L"},{"name":"SAARA (r\u00e9seau Sant\u00e9 Animale Auvergne Rh\u00f4ne Alpes) 2021 project funds and the P\u00f4le EVAAS (P\u00f4le Expertise V\u00e9t\u00e9rinaire et Agronomique Animaux Sauvages) project 1.3"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Rodents are the primary reservoirs for pathogenic Leptospira species, which cause leptospirosis. Among the key potential carriers are water voles, whose population outbreaks can consequently pose a major threat to human and animal health. We studied the prevalence, prominence, and epidemiology of pathogenic Leptospira species in water voles in central France. First, 46 voles were captured, and DNA was extracted from kidney, lung, liver, blood, and urine and tested for the presence of Leptospira using three molecular methods: PCR, O-antigen typing, and variable number tandem repeat (VNTR) typing. We also attempted to culture leptospires from kidney and urine samples. In addition, we investigated leptospiral antibodies in serum samples from 60 sheep using microscopic agglutination testing. These animals co-occurred with the voles, so we sought to assess their degree of exposure and involvement in pathogen dynamics. The overall prevalence of infection was 76.1% (CI95% [61.2%, 87.4%]). The only strain found was L. kirschneri serogroup Grippotyphosa and a similar VNTR profile was acquired. Leptospires were successfully cultured from kidney and urine samples for four voles. Three sheep had low antibody titers against the Leptospira serogroup Grippotyphosa. Taken together, our results suggest the exclusive carriage of L. kirschneri serogroup Grippotyphosa among water voles in central France. Nevertheless, their ability to act as reservoir hosts that transmit the pathogen to co-occurring livestock remains unclear and merits further research.<\/jats:p>","DOI":"10.3390\/pathogens12020260","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T03:34:30Z","timestamp":1675654470000},"page":"260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of Pathogenic Leptospira kirschneri Serogroup Grippotyphosa in Water Voles (Arvicola terrestris) from Ruminant Pastures in Puy-de-D\u00f4me, Central France"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1674-8817","authenticated-orcid":false,"given":"Elena","family":"Harran","sequence":"first","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"},{"name":"Faculty of Arts and Sciences, Holy Spirit University of Kaslik (USEK), Jounieh P.O. Box 446, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Pinot","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeli","family":"Kodjo","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zouheira","family":"Djelouadji","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine","family":"Le Gudayer","sequence":"additional","affiliation":[{"name":"Laboratoire des Leptospires et d\u2019Analyses V\u00e9t\u00e9rinaires, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soro","family":"Sionfoungo Daouda","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Groud","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3371-0952","authenticated-orcid":false,"given":"Virginie","family":"Lattard","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-3765","authenticated-orcid":false,"given":"Florence","family":"Ayral","sequence":"additional","affiliation":[{"name":"USC 1233-RS2GP, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"},{"name":"Laboratoire des Leptospires et d\u2019Analyses V\u00e9t\u00e9rinaires, VetAgro Sup, Universit\u00e9 de Lyon, 69280 Marcy L\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. 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