{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:27:31Z","timestamp":1772252851577,"version":"3.50.1"},"reference-count":60,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CNPq","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"CNPq","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CAPES","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"CAPES","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"FAPEMIG","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"FAPEMIG","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"UFSJ","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"UFSJ","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"UnB","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"UnB","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"UFMG","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"UFMG","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"FAPDF","award":["APQ-408675\/2018-7"],"award-info":[{"award-number":["APQ-408675\/2018-7"]}]},{"name":"FAPDF","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. Methods: a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of L. infantum infection using canine (n = 140) and human (n = 145) sera samples. Results: in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). Conclusions: the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.<\/jats:p>","DOI":"10.3390\/pathogens12020302","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T02:42:33Z","timestamp":1676256153000},"page":"302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis"],"prefix":"10.3390","volume":"12","author":[{"given":"Daniel Silva","family":"Dias","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1293-8365","authenticated-orcid":false,"given":"Juliana Martins","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Aparecida Fernandes","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Sanchez","family":"Machado","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7852-0300","authenticated-orcid":false,"given":"Fernanda Fonseca","family":"Ramos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lais Moreira","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3374-5418","authenticated-orcid":false,"given":"Ana Alice Maia","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia das Intera\u00e7\u00f5es Celulares, Departamento de Morfologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana de Sousa","family":"Ramos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isadora Braga","family":"Gandra","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flaviane Silva","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelli dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonatas Oliveira da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8394-4802","authenticated-orcid":false,"given":"Miguel Angel","family":"Ch\u00e1vez-Fumagalli","sequence":"additional","affiliation":[{"name":"Computational Biology and Chemistry Research Group, Vicerrectorado de Investigaci\u00f3n, Universidad Cat\u00f3lica de Santa Mar\u00eda, Urb. San Jos\u00e9 S\/N, Arequipa 04000, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Gon\u00e7alves","family":"Teixeira-Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Infecto-Parasit\u00e1rias, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Thereza","family":"Chaves","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. 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Prof. Alfredo Balena, 190, Belo Horizonte 30130-100, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-3030","authenticated-orcid":false,"given":"Alexsandro Sobreira","family":"Galdino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Microrganismos, Universidade Federal de S\u00e3o Jo\u00e3o Del-Rei (UFSJ), Campus Centro Oeste, Divin\u00f3polis 35501-296, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, January 11). Leishmaniasis, 2022. Available online: https:\/\/www.who.int\/data\/gho\/data\/themes\/topics\/topic-details\/GHO\/leishmaniasis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Alvar, J., V\u00e9lez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., and Boer, M. 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