{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:51:07Z","timestamp":1789005067617,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,12]],"date-time":"2023-02-12T00:00:00Z","timestamp":1676160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union under the European Social Fund"},{"name":"GHTM\u2013UID\/Multi\/04413\/2013"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Neoehrlichia mikurensis is a new emerging tick-borne Gram-negative bacterium, belonging to the family Anaplasmataceae, the main vector of which in Europe is the tick Ixodes ricinus. N. mikurensis is responsible for neoehrlichiosis, occurring mostly in patients with underlying diseases. In the present study, a total of 348 I. ricinus and Dermacentor reticulatus ticks collected in north-eastern Poland were analyzed for the prevalence of N. mikurensis. A total of 140 questing ticks (124 of I. ricinus ticks and 16 D. reticulatus) collected with the flagging method and 208 ticks (105 and 103 I. ricinus and D. reticulatus, respectively) removed from dogs were selected for the study. cDNA (questing ticks) and total DNA (questing and feeding ticks) were analyzed by qPCR targeting the 16S rRNA gene of N. mikurensis. Positive samples were further analyzed by nested PCR and sequencing. The prevalence differed between ticks collected from vegetation (19.3%; 27\/140) and ticks removed from dogs (6.7%; 14\/208). The presence of the pathogen in questing and feeding D. reticulatus ticks was proven in Poland for the first time. In summary, our research showed that infections of ticks of both the most common tick species I. ricinus and D. reticulatus in north-eastern Poland are present and ticks collected from urban areas were more often infected than ticks from suburban and natural areas. The detection of N. mikurensis in I. ricinus and D. reticulatus ticks from north-eastern Poland indicates potential transmission risk for tick-bitten humans at this latitude.<\/jats:p>","DOI":"10.3390\/pathogens12020307","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T02:42:33Z","timestamp":1676256153000},"page":"307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Neoehrlichia mikurensis\u2014A New Emerging Tick-Borne Pathogen in North-Eastern Poland?"],"prefix":"10.3390","volume":"12","author":[{"given":"Magdalena","family":"Szczotko","sequence":"first","affiliation":[{"name":"Students\u2019 Parasitology \u201cVermis\u201d Science Club, Department of Medical Biology, Collegium Medicum, School of Public Health, University of Warmia and Mazury, 10-719 Olsztyn, Poland"},{"name":"Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-8958","authenticated-orcid":false,"given":"Katarzyna","family":"Kubiak","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Collegium Medicum, School of Public Health, University of Warmia and Mazury in Olsztyn, Zolnierska 14c, 10-561 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142aw Mariusz","family":"Michalski","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Invasive Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7841-3938","authenticated-orcid":false,"given":"Leonardo","family":"Moerbeck","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"},{"name":"Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5512-9093","authenticated-orcid":false,"given":"Sandra","family":"Antunes","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"},{"name":"Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4570-2060","authenticated-orcid":false,"given":"Ana","family":"Domingos","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"},{"name":"Institute of Hygiene and Tropical Medicine, NOVA University, 1349-008 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9986-3847","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Dmitryjuk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-018-3168-y","article-title":"\u201cCandidatus Neoehrlichia Mikurensis\u201d in Ixodes ricinus ticks collected near the Arctic Circle in Norway","volume":"11","author":"Larsson","year":"2018","journal-title":"Parasit. 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