{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T21:01:38Z","timestamp":1780606898095,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,12]],"date-time":"2023-02-12T00:00:00Z","timestamp":1676160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Glaesserella\u00a0(Haemophilus) parasuis, the etiological agent of Gl\u00e4sser\u2019s disease, is an economically significant pathogen commonly associated with serofibrinous polyserositis, arthritis, fibrinous bronchopneumonia and\/or meningitis. This study is the first attempt to molecularly characterize and provide a detailed overview of the genetic variants of G. parasuis present in Malaysia, in reference to its serotype, virulence-associated trimeric autotransporters (vtaA) gene and outer membrane protein P2 (OmpP2) gene. The G. parasuis isolates (n = 11) from clinically sick field samples collected from two major pig producing states (Selangor and Perak) were selected for analysis. Upon multiplex PCR, the majority of the isolates (eight out of 11) were identified to be serotype 5 or 12, and interestingly, serotypes 3, 8 and 15 were also detected, which had never been reported in Malaysia prior to this. Generally, virulent vtaA was detected for all isolates, except for one, which displayed a nonvirulent vtaA. A phylogenetic analysis of the OmpP2 gene revealed that the majority of Malaysian isolates were clustered into genotype 1, which could be further divided into Ia and Ib, while only one isolate was clustered into genotype 2.<\/jats:p>","DOI":"10.3390\/pathogens12020308","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T02:42:33Z","timestamp":1676256153000},"page":"308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Characterization and Phylogenetic Analysis of Outer membrane protein P2 (OmpP2) of Glaesserella (Haemophilus) parasuis Isolates in Central State of Peninsular Malaysia"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6776-226X","authenticated-orcid":false,"given":"Chee Yien","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-2482","authenticated-orcid":false,"given":"Hui Xin","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2674-3959","authenticated-orcid":false,"given":"Chew Yee","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suet Ee","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai Yee","family":"Phang","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2237-1526","authenticated-orcid":false,"given":"Jyhmirn","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine, National Chiayi University, Chiayi City 60004, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1174-0288","authenticated-orcid":false,"given":"Peck Toung","family":"Ooi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6188-0561","authenticated-orcid":false,"given":"Michelle Wai Cheng","family":"Fong","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang 43400, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1099\/ijsem.0.003730","article-title":"Phylogenomic analysis of Haemophilus parasuis and proposed reclassification to Glaesserella parasuis, gen. nov., comb. nov","volume":"70","author":"Dickerman","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.vetmic.2010.04.002","article-title":"Sow vaccination modulates the colonization of piglets by Haemophilus parasuis","volume":"145","author":"Naranjo","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2013.07.027","article-title":"Update on the pathogenesis of Haemophilus parasuis infection and virulence factors","volume":"168","author":"Zhang","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.1128\/JCM.01991-15","article-title":"Development of a multiplex PCR assay for rapid molecular serotyping of Haemophilus parasuis","volume":"53","author":"Howell","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1128\/jcm.30.4.862-865.1992","article-title":"Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts","volume":"30","author":"Kielstein","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.tvjl.2006.10.017","article-title":"Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods","volume":"174","author":"Olvera","year":"2007","journal-title":"Vet. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.vetmic.2009.10.025","article-title":"Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains","volume":"142","author":"Aragon","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1128\/CVI.00168-13","article-title":"Virulence, transmission, and heterologous protection of four isolates of Haemophilus parasuis","volume":"20","author":"Brockmeier","year":"2013","journal-title":"Clin. Vaccine. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Howell, K.J., Weinert, L.A., Chaudhuri, R.R., Luan, S.L., Peters, S.E., Corander, J., Harris, D., Angen, \u00d8., Aragon, V., and Bensaid, A. (2014). The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-1179"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1128\/JCM.39.5.1757-1762.2001","article-title":"Outer membrane proteins and DNA profiles in strains of Haemophilus parasuis recovered from systemic and respiratory sites","volume":"39","author":"Ruiz","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Galofr\u00e9-Mil\u00e0, N., Correa-Fiz, F., Lacouture, S., Gottschalk, M., Strutzberg-Minder, K., Bensaid, A., Pina-Pedrero, S., and Aragon, V. (2017). A robust PCR for the differentiation of potential virulent strains of Haemophilus parasuis. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1041-4"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/s13567-020-00862-1","article-title":"Serotyping and pathotyping of Glaesserella parasuis isolated 2012\u20132019 in Germany comparing different PCR-based methods","volume":"51","author":"Schuwerk","year":"2020","journal-title":"Vet. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.tim.2005.03.004","article-title":"Trimeric autotransporters: A distinct subfamily of autotransporter proteins","volume":"13","author":"Cotter","year":"2005","journal-title":"Trends Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"108595","DOI":"10.1016\/j.vetmic.2020.108595","article-title":"Update on Gl\u00e4sser\u2019s disease: How to control the disease under restrictive use of antimicrobials","volume":"242","author":"Maldonado","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1128\/JB.00703-08","article-title":"Trimeric autotransporters of Haemophilus parasuis: Generation of an extensive passenger domain repertoire specific for pathogenic strains","volume":"191","author":"Pina","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1016\/j.vaccine.2011.01.105","article-title":"Immunogenicity and protection against Haemophilus parasuis infection after vaccination with recombinant virulence associated trimeric autotransporters (VtaA)","volume":"29","author":"Olvera","year":"2011","journal-title":"Vaccine"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetmic.2012.08.019","article-title":"The OmpA family of proteins: Roles in bacterial pathogenesis and immunity","volume":"163","author":"Confer","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.vetimm.2016.09.002","article-title":"Haemophilus parasuis vaccines","volume":"180","author":"Liu","year":"2016","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5988","DOI":"10.1128\/JB.00469-09","article-title":"Characterization and comparative analysis of the genes encoding Haemophilus parasuis outer membrane proteins P2 and P5","volume":"191","author":"Mullins","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5271","DOI":"10.1016\/j.vaccine.2009.06.051","article-title":"Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5","volume":"27","author":"Zhou","year":"2009","journal-title":"Vaccine"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.rvsc.2011.08.019","article-title":"Molecular cloning, sequencing, and expression of the outer membrane protein P2 gene of Haemophilus parasuis","volume":"93","author":"Li","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4236\/eng.2012.410B040","article-title":"The omp2 gene of HPS-type bacteria cloning and sequence analysis isolates from Sichuan Province","volume":"5","author":"Li","year":"2012","journal-title":"Engineering"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.vetmic.2012.01.023","article-title":"Outer membrane protein P2 of the Haemophilus parasuis SC096 strain contributes to adherence to porcine alveolar macrophages cells","volume":"158","author":"Zhang","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.tvjl.2013.12.010","article-title":"The outer membrane protein P2 (OmpP2) of Haemophilus parasuis induces proinflammatory cytokine mRNA expression in porcine alveolar macrophages","volume":"199","author":"Zhou","year":"2014","journal-title":"Vet. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.1128\/JB.186.17.5741-5752.2004","article-title":"Sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompA) of Mannheimia (Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi","volume":"186","author":"Davies","year":"2004","journal-title":"J. Bacteriol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1086\/375724","article-title":"Horizontal transfer of the gene encoding outer membrane protein P2 of nontypeable Haemophilus influenzae, in a patient with chronic obstructive pulmonary disease","volume":"188","author":"Hiltke","year":"2003","journal-title":"J. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2003.12.001","article-title":"Haemophilus parasuis: New trends on diagnosis, epidemiology and control","volume":"99","author":"Oliveira","year":"2004","journal-title":"Vet. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1128\/iai.60.12.5204-5211.1992","article-title":"Molecular analysis of the P2 porin protein of nontypeable Haemophilus influenzae","volume":"60","author":"Sikkema","year":"1992","journal-title":"Infect. Immun."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1292\/jvms.16-0519","article-title":"Genetic variants and phylogenetic analysis of Haemophilus parasuis (HPS) OMPP2 detected in Sichuan, China from 2013 to 2015","volume":"79","author":"Wu","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_32","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"11030","DOI":"10.1073\/pnas.0404206101","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1093\/molbev\/msz312","article-title":"Molecular evolutionary genetics analysis (MEGA) for macOS","volume":"37","author":"Stecher","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_35","first-page":"22","article-title":"Analysis of efficacy obtained with a trivalent inactivated Haemophilus parasuis serovars 4, 5, and 12 vaccine and commercial vaccines against Gl\u00e4sser\u2019s disease in piglets","volume":"81","author":"Zhao","year":"2017","journal-title":"Can. J. Vet. Res."},{"key":"ref_36","unstructured":"Department of Veterinary Services (2023, January 27). List of Approved Veterinary Vaccines, Available online: https:\/\/www.dvs.gov.my\/dvs\/resources\/user_1\/DVS%20pdf\/zooka\/2018\/03.05.2018\/List_of_Approved_Veterinary_Vaccine_updated_May_2018.pdf."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13567-021-00935-9","article-title":"Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR","volume":"52","author":"Macedo","year":"2021","journal-title":"Vet. Res."},{"key":"ref_38","unstructured":"Singh, M.S.G., and Fong, R.W.J. (2014, January 3\u20134). Swine breeding and production in Malaysia. Proceedings of the International Symposium on Recent Progress in Swine Breeding and Raising Technologies, Tainan, Taiwan."},{"key":"ref_39","unstructured":"Choo, L.C. (2017, January 27\u201329). Malaysia Country Report. Proceedings of the OIE Second Regional Workshop on Swine Disease Control, Beijing, China."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1574-6968.2011.02433.x","article-title":"Serum resistance in Haemophilus parasuis SC096 strain requires outer membrane protein P2 expression","volume":"326","author":"Zhang","year":"2012","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Scherrer, S., Rademacher, F., Stephan, R., and Peterhans, S. (2022). Identification of Glaesserella parasuis and Differentiation of Its 15 Serovars Using High-Resolution Melting Assays. Pathogens, 11.","DOI":"10.3390\/pathogens11070752"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/2\/308\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:32:44Z","timestamp":1760121164000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/2\/308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,12]]},"references-count":41,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["pathogens12020308"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12020308","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,12]]}}}