{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:00:44Z","timestamp":1787029244210,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,5]],"date-time":"2023-03-05T00:00:00Z","timestamp":1677974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Demodicosis is one of the most important external parasitic diseases found in carnivores. Three species of the Demodex mite inhabit the skin of dogs and related species, D. canis being the most prevalent. This paper describes the first case of infestation with D. injai in a golden jackal in Romania. An emaciated golden jackal female body found in Timi\u0219 County, western Romania, was examined at Parasitology Department of Faculty of Veterinary Medicine, Timi\u0219oara. The gross lesions were present on different regions of the body: feet, tail, axillary and inguinal areas, and skin folds as well, consisting of erythema, extensive severe alopecia with lichenification, seborrhea, and scaling. In order to establish diagnosis, microscopic examination of skin scrapes, trichogram (hair plucking), acetate tape test (impression), fungal culture, and PCR were performed. Both microscopic measurements and PCR analysis have confirmed the presence of D. injai.<\/jats:p>","DOI":"10.3390\/pathogens12030412","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T03:22:05Z","timestamp":1678072925000},"page":"412","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First Morphological and Molecular Identification of Demodex injai in Golden Jackal (Canis aureus Linnaeus, 1758) in Romania"],"prefix":"10.3390","volume":"12","author":[{"given":"Sorin","family":"Morariu","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9585-1403","authenticated-orcid":false,"given":"Florica","family":"Morariu","sequence":"additional","affiliation":[{"name":"Faculty of Bioengineering of Animal Resources, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9351-4726","authenticated-orcid":false,"given":"Ana-Maria","family":"Marin","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Monica Florina","family":"Moraru","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-Cornel","family":"Popovici","sequence":"additional","affiliation":[{"name":"Forestry Faculty, University Transilvania Brasov, 500036 Bra\u0219ov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3004-463X","authenticated-orcid":false,"given":"Mirela","family":"Imre","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-4641","authenticated-orcid":false,"given":"Violeta","family":"Igna","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisa","family":"Mederle","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/vms3.636","article-title":"The golden jackal (Canis aureus) and the African swine fever pandemic: Its role is controversial but not negligible (a diet analysis study)","volume":"8","author":"Kemenszky","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1186\/s13071-017-2329-8","article-title":"A synoptic overview of golden jackal parasites reveals high diversity of species","volume":"10","author":"Gherman","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1016\/j.cub.2015.06.060","article-title":"Genome-wide evidence reveals that African and Eurasian golden jackals are distinct species","volume":"25","author":"Koepfli","year":"2015","journal-title":"Curr. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1053\/tvjl.2000.0572","article-title":"Antibodies to selected canine pathogens and infestation with intestinal helminths in golden jackals (Canis aureus) in Israel","volume":"162","author":"Shamir","year":"2001","journal-title":"Vet. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1186\/1756-3305-2-S1-S7","article-title":"Wild canids as sentinels of ecological health: A conservation medicine perspective","volume":"2","author":"Aguirre","year":"2009","journal-title":"Parasites Vectors"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1017\/S0022149X00015960","article-title":"Helminths of foxes and other wild carnivores from rural areas in Greece","volume":"71","author":"Papadopoulos","year":"1997","journal-title":"J. Helminthol."},{"key":"ref_7","first-page":"199","article-title":"Role of wild carnivores in the epizootology and epidemiology of trichinellosis","volume":"3","author":"Georgieva","year":"2000","journal-title":"Bulg. J. Vet. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"457","DOI":"10.7589\/0090-3558-44.2.457","article-title":"First identification of Trichinella sp. in golden jackal (Canis aureus) in Romania","volume":"44","author":"Blaga","year":"2008","journal-title":"J. Wildl. Dis."},{"key":"ref_9","first-page":"264","article-title":"Studies on feeding habits and parasitological status of red fox, golden jackal, wild cat and stone marten in Sredna Gora, Bulgaria","volume":"5","author":"Kirkova","year":"2011","journal-title":"J. Life Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1556\/avet.2013.058","article-title":"Data on the parasitological status of golden jackal (Canis aureus L., 1758) in Hungary","volume":"62","author":"Lanszki","year":"2014","journal-title":"Acta Vet. Hung."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1186\/s13071-016-1524-3","article-title":"Role of golden jackals (Canis aureus) as natural reservoirs of Dirofilaria spp. in Romania","volume":"9","author":"Matei","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_12","first-page":"389","article-title":"Endoparasitic fauna of red foxes (Vulpes vulpes) and golden jackals (Canis aureus) in Serbia","volume":"61","author":"Becskei","year":"2016","journal-title":"Acta Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1007\/s00436-011-2703-y","article-title":"Hard ticks (Ixodidae) in Romania: Surveillance, host associations, and possible risks for tick-borne diseases","volume":"110","author":"Dumitrache","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1556\/avet.2012.050","article-title":"Molecular evidence of Ehrlichia canis and Rickettsia massiliae in ixodid ticks of carnivores from South Hungary","volume":"61","author":"Hornok","year":"2013","journal-title":"Acta Vet. Hung."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.ttbdis.2012.10.040","article-title":"A golden jackal (Canis aureus) from Austria bearing Hepatozoon canis-import due to immigration into a non-endemic area?","volume":"4","author":"Duscher","year":"2013","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10493-017-0108-z","article-title":"Ixodid ticks parasitizing wild carnivores in Romania","volume":"71","author":"Dumitrache","year":"2017","journal-title":"Exp. Appl. Acarol."},{"key":"ref_17","first-page":"10","article-title":"Concurrent parasitation with Sarcoptes and Demodex in a golden jackal","volume":"64","author":"Berkovitz","year":"2009","journal-title":"Isr. J. Vet. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3329\/bjvm.v12i2.21295","article-title":"Identifying diseases of golden jackals of Bangladesh Agricultural University campus, Mymensingh, Bangladesh","volume":"12","author":"Yousuf","year":"2014","journal-title":"Bangl. J. Vet. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"433","DOI":"10.7589\/2015-07-196","article-title":"Demodicosis in chamois (Rupicapra rupicapra subsp. rupicapra) in the Italian Alps, 2013\u20132014","volume":"52","author":"Salvadori","year":"2016","journal-title":"J. Wildl. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ringwaldt, E.M., Brook, B.W., Carver, S., and Buettel, J.C. (2021). The patterns and causes of dermatitis in terrestrial and semi-aquatic mammalian wildlife. Animals, 11.","DOI":"10.3390\/ani11061691"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1186\/s13071-017-2234-1","article-title":"A review of Sarcoptes scabiei: Past, present and future","volume":"10","author":"Arlian","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/tbed.14082","article-title":"Sarcoptic mange: An emerging panzootic in wildlife","volume":"69","author":"Escobar","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"367-e83","DOI":"10.1111\/vde.12030","article-title":"A molecular technique for the detection and differentiation of Demodex mites on cats","volume":"24","author":"Frank","year":"2013","journal-title":"Vet. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ilie, M.S., Imre, M., Giubega, S., Luca, I., Florea, T., and Morariu, S. (2021). Feline demodicosis case report-First molecular characterization of Demodex mites in Romania. Pathogens, 10.","DOI":"10.3390\/pathogens10111474"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"487-e80","DOI":"10.1111\/vde.12141","article-title":"PCR amplification and DNA sequence identification of an unusual morphological form of Demodex cati in a cat","volume":"25","author":"Bernstein","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1603\/0022-2585-40.2.146","article-title":"Demodex injai: A new species of hair follicle mite (Acari: Demodecidae) from domestig dog (Canidae)","volume":"40","author":"Desch","year":"2003","journal-title":"J Med. Entomol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"427-e65","DOI":"10.1111\/vde.12136","article-title":"Immunology and pathogenesis of canine demodicosis","volume":"25","author":"Ferrer","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3387","DOI":"10.5433\/1679-0359.2017v38n5p3387","article-title":"The epidemiological and clinical aspects of Demodex injai demodicosis in dogs: A report of eight cases","volume":"38","author":"Paulo","year":"2017","journal-title":"Semin. Cienc. Agrar."},{"key":"ref_29","first-page":"335","article-title":"Demodex sp. (Acari, Demodecidae) and demodecosis in dogs: Characteristics, symptoms, occurrence","volume":"54","author":"Izdebska","year":"2010","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.20546\/ijcmas.2020.912.253","article-title":"Comparative morphometric analysis for differentiation of three Demodex mite species causing canine demodicosis","volume":"9","author":"Chaudhary","year":"2020","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"286-e66","DOI":"10.1111\/vde.12010","article-title":"PCR amplification and DNA sequencing of Demodex injai from otic secretions of a dog","volume":"24","author":"Milosevic","year":"2013","journal-title":"Vet. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"623","DOI":"10.2460\/javma.2002.220.623","article-title":"Large-bodied Demodex mite infestation in 4 dogs","volume":"220","author":"Hillier","year":"2002","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1365-3164.2009.00755.x","article-title":"Demodex injai infestation and dorsal greasy skin and hair in eight wirehaired fox terrier dogs","volume":"20","author":"Ordeix","year":"2009","journal-title":"Vet. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/vde.12183","article-title":"Longitudinal assessment of T-lymphocyte subpopulations during generalized demodicosis in dogs and their relationship with remission","volume":"26","author":"Oliveira","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_35","first-page":"39","article-title":"Study regarding the role and medical and social implications of Demodex spp. parasitism in dogs and humans","volume":"30","author":"Mederle","year":"2020","journal-title":"Rev. Rom. Med. Vet."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/3\/412\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:48:13Z","timestamp":1760122093000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/3\/412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,5]]},"references-count":35,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["pathogens12030412"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12030412","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,5]]}}}