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though previous works showed molecular evidence of hemotropic Mycoplasma spp. (hemoplasmas) in ring-tailed coatis (Nasua nasua) from Brazil, Bartonella sp. has not been reported in these mammals so far. The present study aimed to detect the above-mentioned agents in coatis\u2019 blood and associated ectoparasites, assessing the association between these infections and red blood parameters. Between March 2018 and January 2019, coati (n = 97) blood samples, Amblyomma sp. ticks (2242 individual ticks, resulting in 265 pools), and Neotrichodectes pallidus louse (n = 59) were collected in forested urban areas from midwestern Brazil. DNA extracted from coatis\u2019 blood, and ectoparasite samples were submitted to quantitative PCR (qPCR) (16S rRNA) and conventional PCR (cPCR) (16S rRNA and 23S rRNA) for hemoplasmas and qPCR (nuoG gene) and culturing (only blood) for Bartonella spp. Two different hemoplasma genotypes were detected in blood samples: 71% coatis positive for myc1 and 17% positive for myc2. While 10% of ticks were positive for hemoplasmas (myc1), no louse was positive. The estimated bacterial load of hemoplasmas showed no association with anemia indicators. All coatis were negative for Bartonella sp. in qPCR assay and culturing, albeit two Amblyomma sp. larvae pools, and 2 A. dubitatum nymph pools were positive in the qPCR. The present work showed a high occurrence of hemoplasmas, with two distinct hemoplasma genotypes, in coatis from forested urban areas in midwestern Brazil.<\/jats:p>","DOI":"10.3390\/pathogens12040538","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T02:23:46Z","timestamp":1680143026000},"page":"538","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Survey of Hemotropic Mycoplasma spp. and Bartonella spp. in Coatis (Nasua nasua) from Central-Western Brazil"],"prefix":"10.3390","volume":"12","author":[{"given":"L\u00edvia","family":"Perles","sequence":"first","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s\/n, Zona Rural, Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanessa Teixeira Gomes","family":"Barreto","sequence":"additional","affiliation":[{"name":"Post-Graduation of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande 13471-410, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2032-8129","authenticated-orcid":false,"given":"Filipe Martins","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande 13471-410, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leidiane Lima","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Science, School of Veterinary Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-220, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Carvalho","family":"de Macedo","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande 13471-410, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darci Moraes","family":"Barros-Battesti","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s\/n, Zona Rural, Jaboticabal 14884-900, Brazil"},{"name":"Department of Preventive Veterinary Medicine and Animal Science, School of Veterinary Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-220, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2404-8765","authenticated-orcid":false,"given":"Heitor Miraglia","family":"Herrera","sequence":"additional","affiliation":[{"name":"Post-Graduation of Ecology and Conservation, Mato Grosso do Sul Federal University, Campo Grande 13471-410, Brazil"},{"name":"Laboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande 13471-410, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s\/n, Zona Rural, Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, S\u00e3o Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s\/n, Zona Rural, Jaboticabal 14884-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00779-20","DOI":"10.1128\/AEM.00779-20","article-title":"Hemoplasmas are endemic and cause asymptomatic infection in the endangered Darwin\u2019s fox (Lycalopex fulvipes)","volume":"86","author":"Cevidanes","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3236","DOI":"10.1111\/tbed.13932","article-title":"Worldwide occurrence of haemoplasmas in wildlife: Insights into the patterns of infection, transmission, pathology and zoonotic potential","volume":"68","author":"Volokhov","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_3","first-page":"1","article-title":"Prevalence, Genotype Richness, and Coinfection Patterns of Hemotropic Mycoplasmas in Raccoons (Procyon lotor) on Environmentally Protected and Urbanized Barrier Islands","volume":"83","author":"Volokhov","year":"2017","journal-title":"Appli. Environ. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.actatropica.2017.03.030","article-title":"Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland","volume":"171","author":"Sousa","year":"2017","journal-title":"Acta Trop."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cimid.2017.06.004","article-title":"Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua)","volume":"53","author":"Cubilla","year":"2017","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.1111\/tbed.14311","article-title":"\u2018Candidatus Mycoplasma haematonasua\u2019 and tick-borne pathogens in ring-tailed coatis (Nasua nasua, Linnaeus, 1976) from the Igua\u00e7u National Park, Paran\u00e1 State, southern Brazil","volume":"68","author":"Collere","year":"2021","journal-title":"Transb. Emerg. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1939-165X.2004.tb00342.x","article-title":"Hemotrophic mycoplasmas (hemoplasmas): A review and new insights into pathogenic potential","volume":"33","author":"Messick","year":"2004","journal-title":"Vet. Clin. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1476-4431.2009.00496.x","article-title":"Bartonellosis: An emerging infectious disease of zoonotic importance to animals and human beings","volume":"20","author":"Breitschwerdt","year":"2010","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1128\/CMR.00013-17","article-title":"Bartonella species, an emerging cause of blood-culture-negative endocarditis","volume":"30","author":"Okaro","year":"2017","journal-title":"Clin. Microbial. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1365-2915.2008.00713.x","article-title":"Vector transmission of Bartonella species with emphasis on the potential for tick transmission","volume":"22","author":"Billeter","year":"2008","journal-title":"Med. Vet. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wechtaisong, W., Bonnet, S.I., Lien, Y.Y., Chuang, S.T., and Tsai, Y.L. (2020). Transmission of Bartonella henselae within Rhipicephalus sanguineus: Data on the Potential Vector Role of the Tick. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008664"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.3201\/eid1907.130010","article-title":"Bartonella species in raccoons and feral cats, Georgia, USA","volume":"19","author":"Hwang","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"844","DOI":"10.7589\/2016-01-015","article-title":"Bartonella rochalimae and B. vinsonii subsp. berkhoffii in wild carnivores from colorado, USA","volume":"52","author":"Bai","year":"2016","journal-title":"J. Wildl. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Perles, L., Martins, T.F., Barreto, W.T.G., Carvalho de Macedo, G., Herrera, H.M., Mathias, L.A., Labruna, M.B., Barros-Battesti, D.M., Machado, R.Z., and Andr\u00e9, M.R. (2022). Diversity and Seasonal Dynamics of Ticks on Ring-Tailed Coatis Nasua nasua (Carnivora: Procyonidae) in Two Urban Areas from Midwestern Brazil. Animals, 12.","DOI":"10.3390\/ani12030293"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"389","DOI":"10.2981\/09-104","article-title":"Estimating age of carnivores from the Pantanal region of Brazil","volume":"16","author":"Olifiers","year":"2010","journal-title":"Wildl. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ttbdis.2010.03.002","article-title":"Nymphs of the genus Amblyomma (Acari: Ixodidae) of Brazil: Descriptions, redescriptions, and identification key","volume":"1","author":"Martins","year":"2010","journal-title":"Ticks Tick-borne Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101252","DOI":"10.1016\/j.ttbdis.2019.06.012","article-title":"Ticks (Ixodida: Argasidae, Ixodidae) of Brazil: Updated species checklist and taxonomic keys","volume":"10","author":"Martins","year":"2019","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"375","DOI":"10.7589\/0090-3558-42.2.375","article-title":"Molecular characterization of a Babesia species identified in a North American raccoon","volume":"42","author":"Birkenheuer","year":"2006","journal-title":"J. Wild. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10034","DOI":"10.1073\/pnas.91.21.10034","article-title":"Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences","volume":"91","author":"Black","year":"1994","journal-title":"PNAS"},{"key":"ref_20","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer","year":"1994","journal-title":"Mol. Biol. Biotech."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4049","DOI":"10.1128\/JCM.01478-09","article-title":"Development and application of a universal hemoplasma screening assay based on the SYBR Green PCR principle","volume":"47","author":"Willi","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3237","DOI":"10.1128\/JCM.01125-13","article-title":"Infection with hemotropic Mycoplasma species in patients with or without extensive arthropod or animal contact","volume":"51","author":"Maggi","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","first-page":"1","article-title":"Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil","volume":"23","author":"Mongruel","year":"2020","journal-title":"Braz. J. Vet. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1111\/tbed.13767","article-title":"Genetic diversity of emerging hemotropic mycoplasmas in domestic pigs from Brazil","volume":"68","author":"Sonalio","year":"2021","journal-title":"Transb. Emerg. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1128\/JCM.43.6.2651-2655.2005","article-title":"Novel chemically modified liquid medium that will support the growth of seven Bartonella species","volume":"43","author":"Maggi","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.mimet.2007.01.010","article-title":"A combined approach for the enhanced detection and isolation of Bartonella species in dog blood samples: Pre-enrichment liquid culture followed by PCR and subculture onto agar plates","volume":"69","author":"Duncan","year":"2007","journal-title":"J. Microbiol. Meth."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1128\/JB.182.10.2928-2936.2000","article-title":"Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis","volume":"182","author":"Keim","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_29","first-page":"783","article-title":"Assessment of a quantitative 5\u2032 nuclease real-time polymerase chain reaction using the nicotinamide adenine dinucleotide dehydrogenase gamma subunit (nuoG) for Bartonella species in domiciled and stray cats in Brazil","volume":"18","author":"Dumler","year":"2015","journal-title":"J. Feline. Med. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106037","DOI":"10.1016\/j.actatropica.2021.106037","article-title":"Genetic diversity and Multilocus Sequence Typing Analysis of Bartonella henselae in domestic cats from Southeastern Brazil","volume":"222","author":"Furquim","year":"2021","journal-title":"Acta Trop."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"106742","DOI":"10.1016\/j.actatropica.2022.106742","article-title":"Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil","volume":"237","author":"Dias","year":"2022","journal-title":"Acta Trop."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"Sanger","year":"1977","journal-title":"PNAS"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1101\/gr.8.3.175","article-title":"Base-calling of automated sequencer traces usingPhred. I. Accuracy assessment","volume":"8","author":"Ewing","year":"1998","journal-title":"Genome Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1093\/bioinformatics\/btg180","article-title":"MrBayes 3: Bayesian phylogenetic inference under mixed models","volume":"12","author":"Ronquist","year":"2003","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Stover, B.C., and Muller, K.F. (2010). TreeGraph 2: Combining and visualizing evidence from different phylogenetic analyses. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-7"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Santos, F.M., Macedo, G.C., Barreto, W.T.G., Oliveira-Santos, L.G.R., Garcia, C.M., Miranda Mour\u00e3o, G.D., and Miraglia Herrera, H. (2018). Outcomes of Trypanosoma cruzi and Trypanosoma evansi infections on health of Southern coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis) in the Brazilian Pantanal. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0205613"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jfms.2007.12.007","article-title":"Use of the real-time PCR to detect Mycoplasma haemofelis and \u2018Candidatus Mycoplasma haemominutum\u2019 in the saliva and salivary glands of hemoplasma infected cats","volume":"10","author":"Dean","year":"2008","journal-title":"J. Feline Med. Surg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3714","DOI":"10.1111\/mec.14826","article-title":"Haemoplasmas in wild rodents: Routes of transmission and infection dynamics","volume":"27","author":"Cohen","year":"2018","journal-title":"Mol. Ecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1111\/jvim.15517","article-title":"Evidence for vertical transmission of Mycoplasma haemocanis, but not Ehrlichia ewingii, in a dog","volume":"33","author":"Lashnits","year":"2019","journal-title":"J. Vet. Int. Med."},{"key":"ref_40","first-page":"1","article-title":"Detection of Mycoplasma suis in pre-suckling piglets indicates a vertical transmission","volume":"15","author":"Stadler","year":"2019","journal-title":"BCM Vet. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3347\/kjp.2020.58.5.565","article-title":"Clinical Case of a Transfusion-Associated Canine Mycoplasma haemocanis Infection in the Republic of Korea: A Case Report","volume":"58","author":"Kim","year":"2020","journal-title":"Korean J. Parasitol."},{"key":"ref_42","first-page":"82","article-title":"Density and survivorship of the South American coati (Nasua nasua) in urban areas in Central\u2013Western Brazil","volume":"31","author":"Barreto","year":"2021","journal-title":"Hystrix Italian J. Mammol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1136\/vr.151.3.82","article-title":"Retrospective study of 46 cases of feline haemobartonellosis in Israel and their relationships with FeLV and FIV infections","volume":"151","author":"Harrus","year":"2002","journal-title":"Vet. Rec."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1136\/vr.152.7.193","article-title":"Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and \u2018Candidatus Mycoplasma haemominutum\u2019 in cats in the United Kingdom","volume":"152","author":"Tasker","year":"2003","journal-title":"Vet. Rec."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1292\/jvms.11-0113","article-title":"Molecular identification of \u2018Candidatus Mycoplasma haemovis\u2019 in sheep with hemolytic anemia","volume":"73","author":"Suzuki","year":"2011","journal-title":"J. Vet. Med. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.jfms.2003.12.003","article-title":"Diagnosis of feline haemoplasma infection in Australian cats using a real-time PCR assay","volume":"6","author":"Tasker","year":"2004","journal-title":"J. Feline Med. Surg."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1128\/JCM.44.3.961-969.2006","article-title":"Prevalence, risk factor analysis, and follow-up of infections caused by three feline hemoplasma species in cats in Switzerland","volume":"44","author":"Willi","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.3201\/eid1707.101867","article-title":"Bartonella spp. in Bats, Guatemala","volume":"17","author":"Bai","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1016\/j.meegid.2012.06.009","article-title":"Global distribution and genetic diversity of Bartonella in bat flies (Hippoboscoidea, Streblidae, Nycteribiidae)","volume":"12","author":"Morse","year":"2012","journal-title":"Infec. Genet. Evol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/4\/538\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:06:37Z","timestamp":1760123197000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/4\/538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,30]]},"references-count":49,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["pathogens12040538"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12040538","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,30]]}}}