{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:31:34Z","timestamp":1788906694264,"version":"build-2803163510"},"reference-count":82,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"German Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["13GW0456C"],"award-info":[{"award-number":["13GW0456C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"German Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["13GW0422C"],"award-info":[{"award-number":["13GW0422C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Staphylococcus (S.) aureus colonizes up to 30% of all humans and can occasionally cause serious infections. It is not restricted to humans as it can also often be found in livestock and wildlife. Recent studies have shown that wildlife strains of S. aureus usually belong to other clonal complexes than human strains and that they might differ significantly with regard to the prevalence of genes encoding antimicrobial resistance properties and virulence factors. Here, we describe a strain of S. aureus isolated from a European badger (Meles meles). For molecular characterisation, DNA microarray-based technology was combined with various next-generation sequencing (NGS) methods. Bacteriophages from this isolate were induced with Mitomycin C and characterized in detail by transmission electron microscopy (TEM) and NGS. The S. aureus isolate belonged to ST425 and had a novel spa repeat sequence (t20845). It did not carry any resistance genes. The uncommon enterotoxin gene see was detected in one of its three temperate bacteriophages. It was possible to demonstrate the induction of all three prophages, although only one of them was expected to be capable of excision based on its carriage of the excisionase gene xis. All three bacteriophages belonged to the family Siphoviridae. Minor differences in size and shape of their heads were noted in TEM images. The results highlight the ability of S. aureus to colonize or infect different host species successfully, which can be attributed to a variety of virulence factors on mobile genetic elements, such as bacteriophages. As shown in the strain described herein, temperate bacteriophages not only contribute to the fitness of their staphylococcal host by transferring virulence factors, but also increase mobility among themselves by sharing genes for excision and mobilization with other prophages.<\/jats:p>","DOI":"10.3390\/pathogens12050704","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T09:56:18Z","timestamp":1683885378000},"page":"704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterisation of a Staphylococcus aureus Isolate Carrying Phage-Borne Enterotoxin E from a European Badger (Meles meles)"],"prefix":"10.3390","volume":"12","author":[{"given":"Sindy","family":"Burgold-Voigt","sequence":"first","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4368-8709","authenticated-orcid":false,"given":"Stefan","family":"Monecke","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"},{"name":"Institute for Medical Microbiology and Virology, Dresden University Hospital, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9560-0057","authenticated-orcid":false,"given":"Anne","family":"Busch","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care Medicine, University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Bocklisch","sequence":"additional","affiliation":[{"name":"Thuringian State Authority for Food-Safety and Consumer Protection (TLLV), 99947 Bad Langensalza, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha D.","family":"Braun","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Diezel","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Hotzel","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and Zoonoses, 07751 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth M.","family":"Liebler-Tenorio","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Pathogenesis, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Reinicke","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett","family":"Reissig","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Ruppelt-Lorz","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Virology, Dresden University Hospital, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6612-0043","authenticated-orcid":false,"given":"Ralf","family":"Ehricht","sequence":"additional","affiliation":[{"name":"Leibniz-Institute of Photonic Technology (Leibniz-IPHT), 07745 Jena, Germany"},{"name":"InfectoGnostics Research Campus, 07743 Jena, Germany"},{"name":"Institute of Physical Chemistry, Friedrich Schiller University, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"ref_1","unstructured":"Waldvogel, F.A. (1995). Staphylococcus aureus (Including Toxic Shock Syndrome), Churchill Livingstone."},{"key":"ref_2","first-page":"354","article-title":"Staphylococcus, Micrococcus and other catalase-positive cocci","volume":"Volume 1","author":"Jorgensen","year":"2015","journal-title":"Manual of Clinical Microbiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cimid.2015.11.003","article-title":"Coagulase-positive Staphylococcus isolated from wildlife: Identification, molecular characterization and evaluation of resistance profiles with focus on a methicillin-resistant strain","volume":"44","author":"Nowakiewicz","year":"2016","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_4","first-page":"2222","article-title":"Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife","volume":"68","author":"Loncaric","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/vde.12116","article-title":"mecC- and mecA-positive meticillin-resistant Staphylococcus aureus (MRSA) isolated from livestock sharing habitat with wildlife previously tested positive for mecC-positive MRSA","volume":"25","author":"Loncaric","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Monecke, S., Gavier-Wid\u00e9n, D., Hotzel, H., Peters, M., Guenther, S., Lazaris, A., Loncaric, I., M\u00fcller, E., Reissig, A., and Ruppelt-Lorz, A. (2016). Diversity of Staphylococcus aureus Isolates in European Wildlife. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0168433"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.7589\/2011-10-295","article-title":"Methicillin-resistant Staphylococcus aureus in central Iowa wildlife","volume":"48","author":"Wardyn","year":"2012","journal-title":"J. Wildl. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.vetmic.2012.10.025","article-title":"Association of a lukM-positive clone of Staphylococcus aureus with fatal exudative dermatitis in red squirrels (Sciurus vulgaris)","volume":"162","author":"Simpson","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1136\/vr.b4887","article-title":"Mortality in red squirrels (Sciurus vulgaris) associated with exudative dermatitis","volume":"167","author":"Simpson","year":"2010","journal-title":"Vet. Rec."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.vetmic.2014.02.034","article-title":"Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal","volume":"171","author":"Sousa","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1093\/jac\/dku100","article-title":"Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain","volume":"69","author":"Benito","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Price, L.B., Stegger, M., Hasman, H., Aziz, M., Larsen, J., Andersen, P.S., Pearson, T., Waters, A.E., Foster, J., and Schupp, J. (mBio, 2012). Staphylococcus aureus CC398: Host adaptation and emergence of methicillin resistance in livestock, mBio, in press.","DOI":"10.1128\/mBio.00305-11"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.vetmic.2009.06.014","article-title":"Unexpected sequence types in livestock associated methicillin-resistant Staphylococcus aureus (MRSA): MRSA ST9 and a single locus variant of ST9 in pig farming in China","volume":"139","author":"Wagenaar","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/j.1469-0691.2008.01970.x","article-title":"MRSA in livestock animals-an epidemic waiting to happen?","volume":"14","author":"Wulf","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.vetmic.2015.11.013","article-title":"The clinical impact of livestock-associated methicillin-resistant Staphylococcus aureus of the clonal complex 398 for humans","volume":"200","author":"Becker","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7151","DOI":"10.1128\/AEM.00561-11","article-title":"Characterization of methicillin-resistant Staphylococcus aureus isolates from food and food products of poultry origin in Germany","volume":"77","author":"Kadlec","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19545","DOI":"10.1073\/pnas.0909285106","article-title":"Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus","volume":"106","author":"Lowder","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.vetmic.2018.03.020","article-title":"Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from zoo and wild animals","volume":"218","author":"Thomas","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1111\/lam.12471","article-title":"Experimental studies of microbial populations and incidence of zoonotic pathogens in the faeces of red deer (Cervus elaphus)","volume":"61","author":"Gnat","year":"2015","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Abdullahi, I.N., Fern\u00e1ndez-Fern\u00e1ndez, R., Ju\u00e1rez-Fern\u00e1ndez, G., Mart\u00ednez-\u00c1lvarez, S., Eguiz\u00e1bal, P., Zarazaga, M., Lozano, C., and Torres, C. (2021). Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with \u201cOne Health\u201d Concern. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121556"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.vetmic.2017.06.004","article-title":"High occurrence of mecC-MRSA in wild hedgehogs (Erinaceus europaeus) in Sweden","volume":"207","author":"Bengtsson","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Monecke, S., Gavier-Widen, D., Mattsson, R., Rangstrup-Christensen, L., Lazaris, A., Coleman, D.C., Shore, A.C., and Ehricht, R. (2013). Detection of mecC-positive Staphylococcus aureus (CC130-MRSA-XI) in diseased European hedgehogs (Erinaceus europaeus) in Sweden. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066166"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Rasmussen, S.L., Larsen, J., van Wijk, R.E., Jones, O.R., Berg, T.B., Angen, \u00d8., and Larsen, A.R. (2019). European hedgehogs (Erinaceus europaeus) as a natural reservoir of methicillin-resistant Staphylococcus aureus carrying mecC in Denmark. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222031"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1017\/S0022172400045174","article-title":"Dermatophyte lesions in the hedgehog as a reservoir of penicillin-resistant staphylococci","volume":"63","author":"Smith","year":"1965","journal-title":"J. Hyg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/s41586-021-04265-w","article-title":"Emergence of methicillin resistance predates the clinical use of antibiotics","volume":"602","author":"Larsen","year":"2022","journal-title":"Nature"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Dube, F., S\u00f6derlund, R., Lampinen Salomonsson, M., Troell, K., and B\u00f6rjesson, S. (2021). Benzylpenicillin-producing Trichophyton erinacei and methicillin resistant Staphylococcus aureus carrying the mecC gene on European hedgehogs\u2014A pilot-study. BMC Microbiol., 21.","DOI":"10.1186\/s12866-021-02260-9"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1271\/bbb.68.981","article-title":"Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins: Structures, pore-forming mechanism, and organization of the genes","volume":"68","author":"Kaneko","year":"2004","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1128\/CMR.13.1.16","article-title":"Exotoxins of Staphylococcus aureus","volume":"13","author":"Dinges","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"24394","DOI":"10.1038\/s41598-021-03823-6","article-title":"Staphylococcus aureus isolates from Eurasian Beavers (Castor fiber) carry a novel phage-borne bicomponent leukocidin related to the Panton-Valentine leukocidin","volume":"11","author":"Monecke","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"964358","DOI":"10.3389\/fgene.2022.964358","article-title":"Case Report: Necrotizing fasciitis caused by Staphylococcus aureus positive for a new sequence variant of exfoliative toxin E","volume":"13","author":"Sabat","year":"2022","journal-title":"Front. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1271\/bbb.61.1960","article-title":"Panton-valentine leukocidin genes in a phage-like particle isolated from mitomycin C-treated Staphylococcus aureus V8 (ATCC 49775)","volume":"61","author":"Kaneko","year":"1997","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0378-1119(98)00278-9","article-title":"Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage phiPVL carrying Panton-Valentine leukocidin genes","volume":"215","author":"Kaneko","year":"1998","journal-title":"Gene"},{"key":"ref_33","first-page":"1679","article-title":"Staphylococcus aureus bacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and enterotoxin A: Molecular mechanism of triple conversion","volume":"135","author":"Coleman","year":"1989","journal-title":"J. Gen. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1111\/j.1574-6976.2010.00261.x","article-title":"Lateral genetic transfer and the construction of genetic exchange communities","volume":"35","author":"Skippington","year":"2011","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4594","DOI":"10.1038\/ncomms5594","article-title":"Cooperative secretions facilitate host range expansion in bacteria","volume":"5","author":"McNally","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.tim.2017.05.011","article-title":"Transfer of Antibiotic Resistance in Staphylococcus aureus","volume":"25","author":"Haaber","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"40660","DOI":"10.1038\/srep40660","article-title":"Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus","volume":"7","author":"Koop","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1111\/j.1439-0450.2006.00977.x","article-title":"International dissemination of a high virulence rabbit Staphylococcus aureus clone","volume":"53","author":"Vancraeynest","year":"2006","journal-title":"J. Vet. Med. B Infect. Dis. Vet. Public Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/S1473-3099(11)70126-8","article-title":"Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: A descriptive study","volume":"11","author":"Holden","year":"2011","journal-title":"Lancet Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1128\/AEM.03189-12","article-title":"Genotypic and Phenotypic Characterization of Staphylococcus aureus Isolates from Wild Boars","volume":"79","author":"Meemken","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1111\/1758-2229.12191","article-title":"Detection of mecC-Methicillin-resistant Staphylococcus aureus isolates in river water: A potential role for water in the environmental dissemination","volume":"6","author":"Harrison","year":"2014","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4865","DOI":"10.1128\/AEM.00647-14","article-title":"Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain","volume":"80","author":"Porrero","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s00248-019-01328-4","article-title":"Detection of MRSA of Lineages CC130-mecC and CC398-mecA and Staphylococcus delphini-lnu(A) in Magpies and Cinereous Vultures in Spain","volume":"78","author":"Alonso","year":"2019","journal-title":"Microb. Ecol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Heaton, C.J., Gerbig, G.R., Sensius, L.D., Patel, V., and Smith, T.C. (2020). Staphylococcus aureus Epidemiology in Wildlife: A Systematic Review. Antibiotics, 9.","DOI":"10.3390\/antibiotics9020089"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e1659","DOI":"10.1111\/tbed.14500","article-title":"Survey of Staphylococcus aureus carriage by free-living red deer (Cervus elaphus): Evidence of human and domestic animal lineages","volume":"69","author":"Luzzago","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.3389\/fmicb.2018.01211","article-title":"Molecular Characteristics of Community-Associated Staphylococcus aureus Isolates From Pediatric Patients With Bloodstream Infections Between 2012 and 2017 in Shanghai, China","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Rasmussen, G., Monecke, S., Brus, O., Ehricht, R., and Soderquist, B. (2014). Long term molecular epidemiology of methicillin-susceptible Staphylococcus aureus bacteremia isolates in Sweden. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0114276"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/JCM.00972-11","article-title":"Involvement of methicillin-susceptible Staphylococcus aureus related to sequence type 25 and harboring pvl genes in a case of carotid cavernous fistula after community-associated sepsis","volume":"50","author":"Damasco","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Seijas, C., Mascar\u00f3s, P., Lizana, V., Mart\u00ed-Marco, A., Arnau-Bonachera, A., Chillida-Mart\u00ednez, E., Cardells, J., Selva, L., Viana, D., and Corpa, J.M. (2023). Genomic Characterization of Staphylococcus aureus in Wildlife. Animals, 13.","DOI":"10.3390\/ani13061064"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"596891","DOI":"10.3389\/fmicb.2021.596891","article-title":"Estimation of the Prevalence of Antimicrobial Resistance in Badgers (Meles meles) and Foxes (Vulpes vulpes) in Northern Ireland","volume":"12","author":"Couzens","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1017\/S0950268800030417","article-title":"Tuberculosis: The disease and its epidemiology in the badger, a review","volume":"103","author":"Cheeseman","year":"1989","journal-title":"Epidemiol. Infect."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"105","DOI":"10.20506\/rst.36.1.2614","article-title":"The political economy of bovine tuberculosis in Great Britain","volume":"36","author":"Bennett","year":"2017","journal-title":"Rev. Sci. Tech."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1038\/1841079b0","article-title":"Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C","volume":"184","author":"Otsuji","year":"1959","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","article-title":"Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus","volume":"38","author":"Enright","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5442","DOI":"10.1128\/JCM.41.12.5442-5448.2003","article-title":"Typing of methicillin-mesistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management","volume":"41","author":"Harmsen","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-1-60327-164-6_3","article-title":"Isolation of Phage via Induction of Lysogens","volume":"501","author":"Raya","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"723958","DOI":"10.3389\/fgene.2021.723958","article-title":"Characterisation and molecular analysis of an unusual chimeric methicillin resistant Staphylococcus aureus strain and its bacteriophages","volume":"12","author":"Monecke","year":"2021","journal-title":"Front. Genet."},{"key":"ref_58","unstructured":"Hagemann, R. (1990). Gentechnologische Arbeitsmethoden\u2014Ein Handbuch, Akademie-Verlag."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e00684-15","DOI":"10.1128\/genomeA.00684-15","article-title":"Whole-Genome Sequences of 15 Strains of Staphylococcus aureus subsp. aureus Isolated from Foodstuff and Human Clinical Samples","volume":"3","author":"Crovadore","year":"2015","journal-title":"Genome Announc."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.ijid.2018.11.067","article-title":"Staphylococcus aureus nasal and intestinal carriage by free-ranging red deer: Evidence of human, domestic and wild animal lineages","volume":"79","author":"Luzzago","year":"2019","journal-title":"International. J. Infect. Dis."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3765","DOI":"10.1128\/AAC.00187-11","article-title":"Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus","volume":"55","author":"Shore","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3316","DOI":"10.3390\/v4123316","article-title":"The Staphylococci phages family: An overview","volume":"4","author":"Deghorain","year":"2012","journal-title":"Viruses"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1365-2958.1991.tb00768.x","article-title":"Insertional inactivation of the Staphylococcus aureus beta-toxin by bacteriophage phi 13 occurs by site- and orientation-specific integration of the phi 13 genome","volume":"5","author":"Coleman","year":"1991","journal-title":"Mol. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1128\/JB.188.4.1310-1315.2006","article-title":"The Innate Immune Modulators Staphylococcal Complement Inhibitor and Chemotaxis Inhibitory Protein of Staphylococcus aureus Are Located on {beta}-Hemolysin-Converting Bacteriophages","volume":"188","author":"Rooijakkers","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"O840","DOI":"10.1111\/1469-0691.12679","article-title":"Typing of Panton-Valentine leukocidin-encoding phages carried by methicillin-susceptible and methicillin-resistant Staphylococcus aureus from Italy","volume":"20","author":"Sanchini","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Dokland, T. (2019). Molecular Piracy: Redirection of Bacteriophage Capsid Assembly by Mobile Genetic Elements. Viruses, 11.","DOI":"10.3390\/v11111003"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"6506","DOI":"10.1073\/pnas.1711842115","article-title":"The biomass distribution on Earth","volume":"115","author":"Phillips","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"824","DOI":"10.3201\/eid2105.141920","article-title":"Canine distemper in endangered Ethiopian wolves","volume":"21","author":"Gordon","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/tbed.12445","article-title":"Isolation and Potential for Transmission of Mycobacterium bovis at Human-livestock-wildlife Interface of the Serengeti Ecosystem, Northern Tanzania","volume":"64","author":"Katale","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.vetmic.2005.11.035","article-title":"Wildlife tuberculosis in South African conservation areas: Implications and challenges","volume":"112","author":"Michel","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/iai.4.5.593-595.1971","article-title":"Identification of enterotoxin E","volume":"4","author":"Bergdoll","year":"1971","journal-title":"Infect. Immun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"19528","DOI":"10.2807\/ese.15.13.19528-en","article-title":"First evidence of a food poisoning outbreak due to staphylococcal enterotoxin type E, France, 2009","volume":"15","author":"Ostyn","year":"2010","journal-title":"Eurosurveillance"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/S0140-6736(72)92830-9","article-title":"Food-poisoning due to staphylococcal enterotoxin E","volume":"2","author":"Morris","year":"1972","journal-title":"Lancet"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Monecke, S., Coombs, G., Shore, A.C., Coleman, D.C., Akpaka, P., Borg, M., Chow, H., Ip, M., Jatzwauk, L., and Jonas, D. (2011). A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017936"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.3168\/jds.2018-15373","article-title":"Short communication: Characterization of enterotoxin-producing Staphylococcus aureus isolated from mastitic cows","volume":"102","author":"Grispoldi","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Zhou, Z., Zhang, M., Li, H., Yang, H., Li, X., Song, X., and Wang, Z. (2017). Prevalence and molecular characterization of Staphylococcus aureus isolated from goats in Chongqing, China. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1272-4"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"12127","DOI":"10.1073\/pnas.1405336111","article-title":"Pervasive domestication of defective prophages by bacteria","volume":"111","author":"Bobay","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_78","unstructured":"Dawkins, R. (1994). Das Egoistische Gen (The Selfish Gene, German Ed.), Spektrum Verlag."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"4987","DOI":"10.1128\/IAI.70.9.4987-4996.2002","article-title":"Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus","volume":"70","author":"Peacock","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1128\/AAC.50.1.171-177.2006","article-title":"Ciprofloxacin and trimethoprim cause phage induction and virulence modulation in Staphylococcus aureus","volume":"50","author":"Goerke","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"46319","DOI":"10.1038\/srep46319","article-title":"Staphylococcus sciuri bacteriophages double-convert for staphylokinase and phospholipase, mediate interspecies plasmid transduction, and package mecA gene","volume":"7","author":"Zeman","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Sutcliffe, S.G., Shamash, M., Hynes, A.P., and Maurice, C.F. (2021). Common Oral Medications Lead to Prophage Induction in Bacterial Isolates from the Human Gut. Viruses, 13.","DOI":"10.3390\/v13030455"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/5\/704\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:33:52Z","timestamp":1760124832000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/5\/704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,12]]},"references-count":82,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["pathogens12050704"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12050704","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,12]]}}}