{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:11:00Z","timestamp":1760148660040,"version":"build-2065373602"},"reference-count":40,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n- Facultad de Medicina Veterinaria y Zootecnia, \u201cPrograma de Apoyo al Fortalecimiento de la investigaci\u00f3n,\u201d CONACYT","award":["12-2019"],"award-info":[{"award-number":["12-2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Ticks and tick-borne diseases affect livestock productivity and cause significant economic losses. Therefore, surveillance of these pathogens and vectors is paramount to reducing these effects in livestock. This study aimed to identify Anaplasma marginale and Borrelia burgdorferi sensu lato in ticks collected from cattle. Molecular biology techniques were utilized to identify A. marginale for both types of samples, i.e., ticks and bovine blood. Serology of cattle using indirect immunofluorescence assay (IFA) was conducted to determine antibodies to B. burgdorferi s.l. from seven locations in Nuevo Leon, Mexico, between 2015 and 2017. From 404 bovines, 2880 ticks were collected: Rhipicephalus microplus (2391 females and 395 males), Amblyomma spp. (51 females and 42 males) and Dermacentor\u00a0variabilis (1 female). Rhipicephalus\u00a0microplus represented the largest specimens captured, with 96.7% within the seven study sites. PCR processed only 15% (442) of tick samples to identify A. marginale. Field genera proportions were followed to select testing tick numbers. Results showed that 9.9% (44\/442) of A.\u00a0maginale infected the pooled tick species, whereas the highest percent corresponded to 9.4% (38\/404) in R. microplus. Regarding the molecular analysis of blood samples, 214 of 337 (63.5%) were positive for A.\u00a0maginale. In each of the seven locations, at least one bovine sample tested positive for A.\u00a0maginale. Borrelia\u00a0burgdorferi s.l. was not found either in the ticks or serum samples. Two A.marginale DNA nucleotide sequences obtained in this study were deposited in the GenBank with the following accession numbers OR050501 cattle, and OR050500 R.microplus tick. Results of this work point to current distribution of bovine anaplasmosis in northern Mexico.<\/jats:p>","DOI":"10.3390\/pathogens12060784","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T02:39:47Z","timestamp":1685587187000},"page":"784","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and Serological Identification of Anaplasma marginale and Borrelia burgdorferi in Cattle and Ticks from Nuevo Leon, Northern Mexico"],"prefix":"10.3390","volume":"12","author":[{"given":"Jos\u00e9 \u00c1ngel","family":"Ortiz-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Escobedo 66054, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4807-5146","authenticated-orcid":false,"given":"Jorge Jes\u00fas","family":"Rodr\u00edguez-Rojas","sequence":"additional","affiliation":[{"name":"Unidad de Pat\u00f3genos y Vectores, Centro de Investigaci\u00f3n y Desarrollo en Ciencias de la Salud, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 66460, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas Jaime","family":"Hern\u00e1ndez-Escare\u00f1o","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Escobedo 66054, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3495-0528","authenticated-orcid":false,"given":"Kame-A","family":"Galan-Huerta","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmicay Medicina Molecular, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 66460, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Alfonso","family":"Rebollar-T\u00e9llez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas Laboratorio de Entomolog\u00eda M\u00e9dica, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Moreno-Degollado","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Escobedo 66054, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.","family":"Medina-De la Garza","sequence":"additional","affiliation":[{"name":"Unidad de Pat\u00f3genos y Vectores, Centro de Investigaci\u00f3n y Desarrollo en Ciencias de la Salud, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 66460, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2626-7227","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"S\u00e1nchez-Casas","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Escobedo 66054, NL, Mexico"},{"name":"Unidad de Pat\u00f3genos y Vectores, Centro de Investigaci\u00f3n y Desarrollo en Ciencias de la Salud, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 66460, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8605-9630","authenticated-orcid":false,"given":"Ildefonso","family":"Fern\u00e1ndez-Salas","sequence":"additional","affiliation":[{"name":"Unidad de Pat\u00f3genos y Vectores, Centro de Investigaci\u00f3n y Desarrollo en Ciencias de la Salud, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey 66460, NL, Mexico"},{"name":"Facultad de Ciencias Biol\u00f3gicas Laboratorio de Entomolog\u00eda M\u00e9dica, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, San Nicol\u00e1s de los Garza 66455, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1007\/s00436-015-4698-2","article-title":"Anaplasma marginale and Anaplasma phagocytophilum: Rickettsiales pathogens of veterinary and public health significance","volume":"114","author":"Atif","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.vetpar.2009.09.012","article-title":"The natural history of Anaplasma marginale","volume":"167","author":"Kocan","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1099\/00207713-34-4-496","article-title":"Borrelia burgdorferi sp. nov.: Etiological agent of Lyme disease","volume":"34","author":"Johnson","year":"1984","journal-title":"Int. J. Syst. Bacterio."},{"key":"ref_4","first-page":"253","article-title":"Lyme borreliosis in cattle and horses: A review of the literature","volume":"82","author":"Parker","year":"1992","journal-title":"Cornell. Vet."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Colunga-Salas, P., S\u00e1nchez-Montes, S., Volkow, P., Ru\u00edz-Remigio, A., and Becker, I. (2020). Lyme disease and relapsing fever in Mexico: An overview of human and wildlife infections. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0238496"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"101967","DOI":"10.1016\/j.ttbdis.2022.101967","article-title":"Prevalence of bovine Babesia spp., Anaplasma marginale, and their co-infections in Latin America: Systematic review-meta-analysis","volume":"13","author":"Ferreira","year":"2022","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1016\/j.meegid.2009.09.007","article-title":"Molecular epidemiology of bovine anaplasmosis with a particular focus in Mexico","volume":"9","author":"Ortiz","year":"2009","journal-title":"Infect Genet Evol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.vetpar.2008.08.015","article-title":"Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area","volume":"158","author":"Medrano","year":"2008","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1111\/j.1469-0691.2009.02776.x","article-title":"Demonstration of Borrelia burgdorferi sensu stricto infection in ticks from the northeast of Mexico","volume":"15","author":"Vargas","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_10","unstructured":"Instituto Nacional de Estad\u00edstica, Geograf\u00eda e Inform\u00e1tica (2022, December 05). Encuesta Nacional Agropecuaria. Available online: https:\/\/www.inegi.org.mx\/programas\/ena\/2019\/."},{"key":"ref_11","unstructured":"(2022, December 05). Instituto Nacional de Estad\u00edstica y Geograf\u00eda. Available online: https:\/\/cuentame.inegi.org.mx\/monografias\/informacion\/nl\/territorio\/clima.aspx?tema=me&e=19."},{"key":"ref_12","first-page":"16","article-title":"The Amblyomma (Acari: Ixodida: Ixodidae) of Mexico: Identification keys, distribution and hosts","volume":"2998","author":"Robbins","year":"2011","journal-title":"Zootaxa"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3897\/zookeys.569.7221","article-title":"The Dermacentor (Acari, Ixodida, Ixodidae) of Mexico: Hosts, geographical distribution and new records","volume":"569","author":"Robbins","year":"2016","journal-title":"Zookeys"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11646\/zootaxa.2528.1.1","article-title":"The Argasidae, Ixodidae and Nuttalliellidae (Acari: Ixodida) of the world: A list of valid species names","volume":"2528","author":"Guglielmone","year":"2010","journal-title":"Zootaxa"},{"key":"ref_15","unstructured":"Walker, J.B., Keirans, J.E., and Horak, I.G. (2000). A Guide to the Brown Ticks of the World, Cambridge University Press."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1128\/JCM.39.8.2873-2879.2001","article-title":"Detection and identification of fungal pathogens by PCR and ITS2 and 5.8 S ribosomal DNA typing in ocular infections","volume":"39","author":"Ferrer","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/JCM.38.5.1895-1900.2000","article-title":"Direct molecular typing of Borrelia burgdorferi sensu lato species in synovial samples from patients with Lyme arthritis","volume":"38","author":"Jaulhac","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.exppara.2012.11.005","article-title":"Development of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle","volume":"133","author":"Simuunza","year":"2013","journal-title":"Exp. Parasitol."},{"key":"ref_19","unstructured":"Centers for Disease Control and Prevention (2023, January 09). Division of Health Informatics & Surveillance (DHIS), Center for Surveillance, Epidemiology & Laboratory Services (CSELS), Available online: http:\/\/www.cdc.gov\/epiinfo."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Casta\u00f1eda-Ortiz, E.J., Ueti, M.W., Camacho-Nuez, M., Mosqueda, J.J., Mousel, M.R., Johnson, W.C., and Palmer, G.H. (2015). Association of Anaplasma marginale strain superinfection with infection prevalence within tropical regions. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0129415"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/B:TROP.0000012105.19518.80","article-title":"The effect of management factors on the seroprevalence of Anaplasma marginale in Bos indicus cattle in the Mexican tropics","volume":"36","author":"Wagner","year":"2004","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.vaccine.2006.07.049","article-title":"Anaplasma marginale field challenge: Protection by an inactivated immunogen that shares partial sequence of msp1\u03b1 variable region with the challenge strain","volume":"25","author":"Vega","year":"2007","journal-title":"Vaccine"},{"key":"ref_23","first-page":"88","article-title":"Anaplasmosis and babesiosis prevalence in the Experimental Station of Aldama, Tamaulipas","volume":"46","year":"1984","journal-title":"T\u00e9cnica Pecu. M\u00e9xico"},{"key":"ref_24","first-page":"133","article-title":"Prevalencia de anticuerpos contra Anaplasma marginale y Babesia spp. en la zona centro del estado de Guerrero","volume":"47","author":"Fragoso","year":"1984","journal-title":"Rev. Mex Cienc. Pecu"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0167-5877(97)00016-0","article-title":"Bovine anaplasmosis prevalence in northern Veracruz state, Mexico","volume":"32","author":"Rodriguez","year":"1997","journal-title":"Prev. Vet. Med."},{"key":"ref_26","first-page":"38","article-title":"Seroprevalencia de anaplasmosis en explotaciones bovinas de 18 municipios de la zona norte de Veracruz","volume":"34","author":"Rojas","year":"1996","journal-title":"Rev. Mex Cienc. Pecu"},{"key":"ref_27","first-page":"85","article-title":"Anaplasmosis bovina con \u00e9nfasis en control, diagnostico, distribuci\u00f3n de la enfermedad en M\u00e9xico y uso de una vacuna atenuada de Anaplasma marginale","volume":"8","author":"Osorno","year":"1977","journal-title":"Vet. M\u00e9xico"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s10493-006-7251-2","article-title":"The distribution and ecological preferences of Boophilus microplus (Acari: Ixodidae) in Mexico","volume":"38","year":"2006","journal-title":"Exp. Appl. Acarol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10493-018-0302-7","article-title":"Differential expression analysis for subolesin in Rhipicephalus microplus infected with Anaplasma marginale","volume":"76","author":"Contreras","year":"2018","journal-title":"Exp. Appl. Acarol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Estrada-Pe\u00f1a, A., Szab\u00f3, M., Labruna, M., Mosqueda, J., Merino, O., Tarragona, E., Venzal, J.M., and de la Fuente, J. (2020). Towards an effective, rational and sustainable approach for the control of cattle ticks in the Neotropics. Vaccines, 8.","DOI":"10.3390\/vaccines8010009"},{"key":"ref_31","first-page":"117","article-title":"Enfermedad de Lyme. Informe de dos casos","volume":"51","author":"Arroyave","year":"1994","journal-title":"Bol. Med. Hosp. Infant. Mex"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1590\/S0036-36342003000500004","article-title":"Estudio seroepidemiol\u00f3gico de borreliosis de Lyme en la Ciudad de M\u00e9xico y el noreste de la Rep\u00fablica Mexicana","volume":"45","author":"Torres","year":"2003","journal-title":"Salud. Publ. Mex"},{"key":"ref_33","first-page":"105","article-title":"Evidencia de la enfermedad de Lyme en una poblaci\u00f3n de alto riesgo del noreste de M\u00e9xico","volume":"9","author":"Garza","year":"2007","journal-title":"Med. Univer."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"97","DOI":"10.5114\/reum.2016.61208","article-title":"Antibody profile to Borrelia burgdorferi in veterinarians from Nuevo Le\u00f3n, Mexico, a non-endemic area of this zoonosis","volume":"54","author":"Flores","year":"2016","journal-title":"Rheumatology"},{"key":"ref_35","first-page":"7","article-title":"Detection of Borrelia burgdorferi DNA in human skin biopsies and dog synovial fluid by the polymerase chain reaction","volume":"37","year":"1995","journal-title":"Rev. Latinoam. Microbiol."},{"key":"ref_36","first-page":"1","article-title":"Serological survey of canine borreliosis","volume":"41","year":"1999","journal-title":"Rev. Latinoam. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1089\/vbz.2019.2600","article-title":"Molecular detection of Leptospira interrogans and Borrelia burgdorferi in wild rodents from Mexico","volume":"20","year":"2020","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s10493-013-9707-5","article-title":"Distribution of ixodid ticks on dogs in Nuevo Le\u00f3n, Mexico, and their association with Borrelia burgdorferi sensu lato","volume":"61","year":"2013","journal-title":"Exp. Appl. Acarol."},{"key":"ref_39","first-page":"373386","article-title":"Diversidad gen\u00e9tica de la regi\u00f3n variable de los genes msp1a y msp4 en cepas de Anaplasma marginale de M\u00e9xico","volume":"3","year":"2012","journal-title":"Rev. Mex Cienc. Pecu"},{"key":"ref_40","first-page":"5902029","article-title":"Whole-genome sequencing of mexican strains of Anaplasma marginale: An approach to the causal agent of bovine anaplasmosis","volume":"2020","year":"2020","journal-title":"Int. J. Genom."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/6\/784\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:46:01Z","timestamp":1760125561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/6\/784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,31]]},"references-count":40,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["pathogens12060784"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12060784","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2023,5,31]]}}}