{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:54:31Z","timestamp":1765961671508,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>African swine fever (ASF) is a viral disease of swine with a huge impact due to its high mortality. Lately, the disease has actively spread around the world, affecting new areas from which it had been eradicated long ago. To date, ASF control is carried out by the implementation of strict biosecurity measures such as the early identification of infected animals. In this work, two fluorescent rapid tests were developed to improve the sensitivity of point-of-care diagnosis of ASF. For antigen (Ag) detection in blood, a double-antibody sandwich fluorescent lateral flow assay (LFA) was developed, employing a newly developed recombinant antibody to the VP72 of the virus. To complement the diagnosis, a double-recognition fluorescent LFA was developed using the VP72 for the detection of specific antibodies (Ab) in sera or blood. Both assays statistically improved the detection of the disease when compared to the commercial colorimetric assays INgezim\u00ae ASFV CROM Ag and INgezim\u00ae PPA CROM Anticuerpo, respectively, with higher statistical significance between 11 and 39 days post-infection. From the observation of results, it can be concluded that the combination of both Ag-LFA and Ab-LFA assays would facilitate the identification of infected animals, regardless of post-infection time.<\/jats:p>","DOI":"10.3390\/pathogens12060811","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T02:58:32Z","timestamp":1686193112000},"page":"811","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Improving African Swine Fever Surveillance Using Fluorescent Rapid Tests"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-2544","authenticated-orcid":false,"given":"Cristina","family":"Aira","sequence":"first","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Monedero","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Hern\u00e1ndez-Ant\u00f3n","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Mart\u00ednez-Cano","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Camu\u00f1as","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Casado","sequence":"additional","affiliation":[{"name":"European Union Reference Laboratory for ASF, Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA\/CSIC), Carretera Algete-El Casar de Talamanca, Km. 8.1, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Nieto","sequence":"additional","affiliation":[{"name":"European Union Reference Laboratory for ASF, Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA\/CSIC), Carretera Algete-El Casar de Talamanca, Km. 8.1, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmina","family":"Gallardo","sequence":"additional","affiliation":[{"name":"European Union Reference Laboratory for ASF, Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA\/CSIC), Carretera Algete-El Casar de Talamanca, Km. 8.1, 28130 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marga","family":"Garc\u00eda-Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paloma","family":"Rueda","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-8522","authenticated-orcid":false,"given":"Alba","family":"Fresco-Taboada","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos Garc\u00eda Noblejas 39, 28037 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s40813-015-0013-y","article-title":"African Swine Fever: A Global View of the Current Challenge","volume":"1","author":"Gallardo","year":"2015","journal-title":"Porc. 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