{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:04:37Z","timestamp":1791597877375,"version":"4.3.4"},"reference-count":73,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,17]],"date-time":"2023-06-17T00:00:00Z","timestamp":1686960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The prevalence of Listeria monocytogenes in bovine bulk-tank milk (BTM) in Greece has not been previously investigated. The aim of the study was to estimate the prevalence of L. monocytogenes in bovine BTM in Greece and to characterize the isolates in terms of carriage of genes encoding for pathogenic determinants, assess the isolates\u2019 biofilm-forming ability and determine their susceptibility against 12 antimicrobials. Samples (n = 138) of bovine BTM were obtained from farms located throughout Northern Greece and were analyzed qualitatively and quantitatively for L. monocytogenes. Five samples (3.6%) tested positive for L. monocytogenes. The pathogen\u2019s populations in these positive samples were below 5 CFU\/mL. Most isolates belonged to the molecular serogroup \u201c1\/2a, 3a\u201d. All isolates carried the virulence genes inlA, inlC, inlJ, iap, plcA and hlyA, but actA was detected in only three isolates. The isolates displayed weak to moderate biofilm-forming ability and distinct antimicrobial resistance profiles. All isolates were characterized as multidrug resistant, with resistance to penicillin and clindamycin being a common feature. Considering that L. monocytogenes constitutes a serious public health threat, the key findings of the study, related to the carriage of virulence genes and multidrug resistance, highlight the importance of continued monitoring of the pathogen in farm animals.<\/jats:p>","DOI":"10.3390\/pathogens12060837","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T03:04:40Z","timestamp":1687143880000},"page":"837","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece"],"prefix":"10.3390","volume":"12","author":[{"given":"Apostolos S.","family":"Angelidis","sequence":"first","affiliation":[{"name":"Laboratory of Safety and Quality of Milk and Dairy Products, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afroditi S.","family":"Grammenou","sequence":"additional","affiliation":[{"name":"Laboratory of Hygiene of Foods of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8804-239X","authenticated-orcid":false,"given":"Charalampos","family":"Kotzamanidis","sequence":"additional","affiliation":[{"name":"Hellenic Agricultural Organization-DIMITRA, Veterinary Research Institute of Thessaloniki, 57001 Thermi, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-3627","authenticated-orcid":false,"given":"Nektarios D.","family":"Giadinis","sequence":"additional","affiliation":[{"name":"Clinic of Farm Animals, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonios G.","family":"Zdragas","sequence":"additional","affiliation":[{"name":"Hellenic Agricultural Organization-DIMITRA, Veterinary Research Institute of Thessaloniki, 57001 Thermi, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Sergelidis","sequence":"additional","affiliation":[{"name":"Laboratory of Hygiene of Foods of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0044222","DOI":"10.1128\/spectrum.00442-22","article-title":"Soil collected in the Great Smoky Mountains National Park yielded a novel Listeria sensu stricto species, L. swaminathanii","volume":"10","author":"Carlin","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/ijsem.0.005437","article-title":"Listeria ilorinensis sp. Nov., isolated from cow milk cheese in Nigeria","volume":"72","author":"Raufu","year":"2022","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1469-0691.2009.03109.x","article-title":"Listeriosis: A resurgent foodborne infection","volume":"16","author":"Allerberger","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_4","unstructured":"National Public Health Organization (NPHO) (2023, April 17). Epidemiological Data for Listeriosis in Greece 2004\u20132021 Mandatory Notification System, Available online: https:\/\/eody.gov.gr\/wp-content\/uploads\/2023\/01\/epidemiological-data-for-listeriosis-greece-2004-2021.pdf."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC) (2022). The European Union One Health 2021 Zoonoses Report. EFSA J., 20, e07666.","DOI":"10.2903\/j.efsa.2022.7666"},{"key":"#cr-split#-ref_6.1","unstructured":"European Commission (2005). Commission Regulation"},{"key":"#cr-split#-ref_6.2","unstructured":"(EC) No 2073\/2005 of 15 November 2005 on microbiological criteria for foodstuffs. OJ L, 338, 1-26."},{"key":"ref_7","unstructured":"EFSA (2023, March 21). Listeria monocytogenes Story Map. Available online: https:\/\/storymaps.arcgis.com\/stories\/629e6627e6c64111bfd5b9257473c74a."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0168-1605(96)01105-1","article-title":"Listeria monocytogenes in bovine mastitis. Possible implication for human health","volume":"32","author":"Jensen","year":"1996","journal-title":"Int. J. Food Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1099\/00222615-43-2-125","article-title":"Bovine mastitis caused by Listeria monocytogenes: Characteristics of natural and experimental infections","volume":"43","author":"Bourry","year":"1995","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.3168\/jds.2019-16267","article-title":"Persistent contamination by Listeria monocytogenes of bovine raw milk investigated by whole-genome sequencing","volume":"102","author":"Ricchi","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0958-6946(92)90016-F","article-title":"On the origin of Listeria monocytogenes in raw bulk-tank milk","volume":"2","author":"Fedio","year":"1992","journal-title":"Int. Dairy J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.3168\/jds.S0022-0302(93)77628-6","article-title":"Risk factors associated with contamination of raw milk by Listeria monocytogenes in dairy farms","volume":"76","author":"Sanaa","year":"1993","journal-title":"J. Dairy Sci."},{"key":"ref_13","unstructured":"World Health Organization (WHO) Working Group (1998). Foodborne listeriosis. Bull. World Health Organ., 66, 421\u2013428."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.ijfoodmicro.2018.05.015","article-title":"Foodborne pathogens in unpasteurized milk in Sweden","volume":"284","author":"Artursson","year":"2018","journal-title":"Int. J. Food Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"EFSA, and ECDC (2016). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. EFSA J., 14, e04634.","DOI":"10.2903\/j.efsa.2016.4634"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-1605(02)00005-3","article-title":"Elevated carnitine accumulation by Listeria monocytogenes impaired in glycine betaine transport is insufficient to restore wild-type cryotolerance in milk whey","volume":"75","author":"Angelidis","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_17","unstructured":"(2017). Microbiology of the Food Chain\u2014Horizontal Method for the Detection and Enumeration of Listeria monocytogenes and of Listeria spp.\u2014Part 1: Detection Method (Standard No. ISO 11290-1)."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the major Listeria monocytogenes serovars by multiplex PCR","volume":"42","author":"Doumith","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1111\/jam.14244","article-title":"Characterization of Listeria monocytogenes from encephalitis cases of small ruminants from different geographical regions, in Greece","volume":"126","author":"Kotzamanidis","year":"2019","journal-title":"J. Appl. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.mimet.2007.08.007","article-title":"A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes","volume":"71","author":"Liu","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ijfoodmicro.2006.06.029","article-title":"Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases","volume":"113","author":"Rawool","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2018). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [28th ed.]. CLSI supplement M100."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/j.1472-765X.2010.02843.x","article-title":"Antibiotic resistance in Listeria species isolated from catfish fillets and processing environment","volume":"50","author":"Chen","year":"2010","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_25","first-page":"2566","article-title":"Prevalence of virulence genes and biofilm formation among Staphylococcus aureus clinical isolates associated with lower respiratory infection","volume":"4","author":"Wang","year":"2010","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s10482-011-9666-y","article-title":"Survival and biofilm formation by Group B streptococci in simulated vaginal fluid at different pHs","volume":"101","author":"Borges","year":"2012","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.foodcont.2013.01.004","article-title":"Monitoring of foodborne pathogenic bacteria in vending machine raw milk in Piedmont, Italy","volume":"32","author":"Bianchi","year":"2013","journal-title":"Food Control"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.foodcont.2015.08.019","article-title":"Survey of prevalence and seasonal variability of Listeria monocytogenes in raw cow milk from Northern Italy","volume":"60","author":"Dalzini","year":"2016","journal-title":"Food Control"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1111\/jfs.12265","article-title":"Prevalence of Listeria spp. and Listeria monocytogenes in cattle farms in Cyprus using bulk tank milk samples","volume":"36","author":"Botsaris","year":"2016","journal-title":"J. Food Saf."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3855\/jidc.8256","article-title":"The prevalence of Listeria monocytogenes and Staphylococcus aureus and their virulence genes in bulk tank milk in Kosovo","volume":"11","author":"Mehmeti","year":"2017","journal-title":"J. Infect. Dev. Ctries"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1089\/fpd.2012.1284","article-title":"Pathogenic bacteria in Finnish bulk tank milk","volume":"10","author":"Ruusunen","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.foodcont.2012.06.047","article-title":"Listeria monocytogenes prevalence and serotype diversity in various foods","volume":"30","author":"Kramarenko","year":"2013","journal-title":"Food Control"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2017.08.017","article-title":"Occurrence and growth of Listeria monocytogenes in packaged raw milk","volume":"261","author":"Castro","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1472-765X.1995.tb00407.x","article-title":"The incidence, numbers and types of Listeria monocytogenes isolated from farm bulk tank milks","volume":"20","author":"Fenlon","year":"1995","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0168-1605(02)00117-4","article-title":"Estimation of low bacterial concentration: Listeria monocytogenes in raw milk","volume":"80","author":"Diot","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1471-0307.1995.tb02433.x","article-title":"The incidence of Salmonella and Listeria in raw milk from farm bulk tanks in England and Wales","volume":"48","year":"1995","journal-title":"J. Soc. Dairy Technol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3366","DOI":"10.1128\/AEM.68.7.3366-3370.2002","article-title":"Prevalence and fingerprinting of Listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks","volume":"68","author":"Waak","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Varsaki, A., Ortiz, S., Santorum, P., L\u00f3pez, P., L\u00f3pez-Alonso, V., Hern\u00e1ndez, M., Abad, D., Rodr\u00edguez-Grande, J., Ocampo-Sosa, A.A., and Mart\u00ednez-Su\u00e1rez, J.V. (2022). Prevalence and population diversity of Listeria monocytogenes isolated from dairy cattle farms in the Cantabria region of Spain. Animals, 12.","DOI":"10.3390\/ani12182477"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"435187","DOI":"10.5402\/2012\/435187","article-title":"Incidence of Listeria spp. in dairy cows feed and raw milk in Latvia","volume":"2012","author":"Konosonoka","year":"2012","journal-title":"ISRN Vet. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4283","DOI":"10.1038\/s41467-019-12072-1","article-title":"A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates","volume":"10","author":"Yin","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.4315\/0362-028X-68.12.2648","article-title":"Multicenter validation of a multiplex PCR assay for differentiating the major Listeria monocytogenes serovars 1\/2a, 1\/2b, 1\/2c and 4b: Toward an international standard","volume":"68","author":"Doumith","year":"2005","journal-title":"J. Food Prot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.4315\/0362-028X.JFP-14-072","article-title":"Listeria monocytogenes serotype prevalence and biodiversity in diverse food products","volume":"77","author":"Hadjilouka","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/lam.13620","article-title":"Prevalence and characteristics of Listeria monocytogenes in meat, meat products, food handlers and the environment of the meat processing and the retail facilities of a company in Northern Greece","volume":"74","author":"Papatzimos","year":"2021","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Andritsos, N.D., and Mataragas, M. (2023). Characterization and antibiotic resistance of Listeria monocytogenes strains isolated from Greek Myzithra soft whey cheese and related food processing surfaces over two-and-a-half years of safety monitoring in a cheese processing facility. Foods, 12.","DOI":"10.3390\/foods12061200"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1089\/fpd.2012.1187","article-title":"High level of heterogeneity among Listeria monocytogenes isolates from clinical and food origin specimens in Greece","volume":"9","author":"Houhoula","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.foodcont.2013.04.023","article-title":"Prevalence, characterization and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks","volume":"34","author":"Jamali","year":"2013","journal-title":"Food Control"},{"key":"ref_47","first-page":"184","article-title":"The prevalence and molecular characterization of Listeria monocytogenes in corn silage, feces and bulk tank milk sample in dairy cattle farms in Balikesir, Turkey","volume":"74","author":"Aydin","year":"2019","journal-title":"Isr. J. Vet. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.3168\/jds.2017-13546","article-title":"Prevalence of Salmonella enterica, Listeria monocytogenes, and pathogenic Escherichia coli in bulk tank milk and milk filters from US dairy operations in the National Animal Health Monitoring System dairy 2014 study","volume":"101","author":"Sonnier","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1089\/fpd.2016.2118","article-title":"Prevalence, virulence potential, and antibiotic susceptibility profile of Listeria monocytogenes isolated from bovine raw milk samples obtained from Rajasthan, India","volume":"14","author":"Sharma","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.4315\/0362-028X-65.11.1811","article-title":"Listeria monocytogenes virulence and pathogenicity, a food safety perspective","volume":"65","author":"Kathariou","year":"2002","journal-title":"J. Food Prot."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1089\/fpd.2017.2419","article-title":"Implicated food products for listeriosis and changes in serovars of Listeria monocytogenes affecting humans in recent decades","volume":"15","author":"Tham","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e0006019","DOI":"10.1128\/cmr.00060-19","article-title":"Human listeriosis","volume":"36","author":"Koopmans","year":"2023","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/j.tim.2021.01.008","article-title":"Making sense of the biodiversity and virulence of Listeria monocytogenes","volume":"29","author":"Disson","year":"2021","journal-title":"Trends Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"792162","DOI":"10.3389\/fmicb.2021.792162","article-title":"Different shades of Listeria monocytogenes: Strain, serotype, and lineage-based variability in virulence and stress tolerance profiles","volume":"12","author":"Muchaamba","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.meegid.2008.04.006","article-title":"Lineage specific recombination and positive selection in coding and intragenic regions contributed to evolution of the main Listeria monocytogenes virulence gene cluster","volume":"8","author":"Orsi","year":"2008","journal-title":"Infect. Genet. Evol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.3389\/fmicb.2018.01103","article-title":"Virulence gene sequencing highlights similarities and differences in sequences in Listeria monocytogenes serotype 1\/2a and 4b strains of clinical and food origin from 3 different geographic locations","volume":"9","author":"Poimenidou","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Owusu-Kwarteng, J., Wuni, A., Akabanda, F., and Jespersen, L. (2018). Prevalence and characteristics of Listeria monocytogenes isolates in raw milk, heated milk and nunu, a spontaneously fermented milk beverage, in Ghana. Beverages, 4.","DOI":"10.3390\/beverages4020040"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Kayode, A.J., and Okoh, A.I. (2022). Assessment of multidrug-resistant Listeria monocytogenes in milk and milk product and One Health Perspective. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0270993"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"109419","DOI":"10.1016\/j.lwt.2020.109419","article-title":"Molecular serogrouping and virulence of Listeria monocytogenes from local dairy cattle farms and beef in Jordan","volume":"127","author":"Obaidat","year":"2020","journal-title":"LWT"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Doijad, S.P., Barbuddhe, S.B., Garg, S., Poharkar, K.V., Kalorey, D.R., Kurkure, N.V., Rawool, D.B., and Chakraborty, T. (2015). Biofilm-forming abilities of Listeria monocytogenes serotypes isolated from different sources. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137046"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"109784","DOI":"10.1016\/j.ijfoodmicro.2022.109784","article-title":"Biofilm formation and genomic features of Listeria monocytogenes strains isolated from meat and dairy industries located in Piedmont (Italy)","volume":"378","author":"Rubiola","year":"2022","journal-title":"Int. J. Food Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"109424","DOI":"10.1016\/j.foodres.2020.109424","article-title":"Strain variability in biofilm formation: A food safety and quality perspective","volume":"137","author":"Lianou","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.3168\/jds.2009-2717","article-title":"Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes","volume":"93","author":"Latorre","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/S1473-3099(16)30521-7","article-title":"Clinical features and prognostic factors of listeriosis: The MONALISA national prospective cohort study","volume":"17","author":"Charlier","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/CMR.10.2.345","article-title":"Management of listeriosis","volume":"10","author":"Hof","year":"1997","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.fct.2018.02.006","article-title":"The prevalence and antimicrobial resistance of Listeria spp in raw milk and traditional dairy products delivered in Yazd, central Iran (2016)","volume":"114","author":"Derakhshan","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/jfs.12208","article-title":"Prevalence, antimicrobial resistance and serotype distribution of Listeria monocytogenes isolated from raw milk and dairy products","volume":"36","author":"Kevenk","year":"2016","journal-title":"J. Food Saf."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1111\/1541-4337.12387","article-title":"Emergence of antibiotic resistance in Listeria monocytogenes isolated from food products: A comprehensive review","volume":"17","author":"Olaimat","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"4458","DOI":"10.1128\/AEM.70.8.4458-4467.2004","article-title":"Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment","volume":"70","author":"Nightingale","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"107540","DOI":"10.1016\/j.foodcont.2020.107540","article-title":"Listeria monocytogenes dissemination in farming and primary production: Sources, shedding and control measures","volume":"120","author":"Rodriguez","year":"2021","journal-title":"Food Control"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Lianou, D.T., Skoulakis, A., Michael, C.K., Katsarou, E.I., Chatzopoulos, D.C., Solomakos, N., Tsilipounidaki, K., Florou, Z., Cripps, P.J., and Katsafadou, A.I. (2022). Isolation of Listeria ivanovii from bulk-tank milk of sheep and goat farms\u2014From clinical work to bioinformatics studies: Prevalence, association with milk quality, antibiotic susceptibility, predictors, whole genome sequence and phylogenetic relationships. Biology, 11.","DOI":"10.3390\/biology11060871"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2488","DOI":"10.1038\/s41467-019-10380-0","article-title":"Hypervirulent Listeria monocytogenes clones\u2019 adaption to mammalian gut accounts for their association with dairy products","volume":"10","author":"Maury","year":"2019","journal-title":"Nat. Commun."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/6\/837\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:56:55Z","timestamp":1760126215000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/6\/837"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,17]]},"references-count":73,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["pathogens12060837"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12060837","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,17]]}}}