{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:22:17Z","timestamp":1780413737264,"version":"3.54.1"},"reference-count":73,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Centre Pasteur Cameroon (Call for proposals for internal projects of the Centre Pasteur of Cameroon 2019","award":["N\u00b0012\/2015\/CPC\/DS"],"award-info":[{"award-number":["N\u00b0012\/2015\/CPC\/DS"]}]},{"name":"French Institute for Sustainable Development (IRD)","award":["N\u00b0012\/2015\/CPC\/DS"],"award-info":[{"award-number":["N\u00b0012\/2015\/CPC\/DS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Intermittent preventive treatment in pregnancy with sulfadoxine and pyrimethamine (IPTp-SP) is a key component in the malaria control strategy implemented in Africa. The aim of this study was to determine IPTp-SP adherence and coverage, and the impact on maternal infection and birth outcomes in the context of widespread SP resistance in the city of Douala, Cameroon. Clinical and demographic information were documented among 888 pregnant women attending 3 health facilities, from the antenatal care visit to delivery. Positive samples were genotyped for P. falciparum gene (dhfr, dhps, and k13) mutations. The overall IPTp-SP coverage (\u2265three doses) was 17.5%, and 5.1% received no dose. P. falciparum prevalence was 16%, with a predominance of submicroscopic infections (89.3%). Malaria infection was significantly associated with locality and history of malaria, and it was reduced among women using indoor residual spraying. Optimal doses of IPTp-SP were significantly associated with reduced infection among newborns and women (secundiparous and multiparous), but there was no impact of IPTp-SP on the newborn bodyweight. Pfdhfr-Pfdhps quintuple mutants were over-represented (IRNI-FGKAA, IRNI-AGKAA), and sextuple mutants (IRNI-AGKAS, IRNI-FGEAA, IRNI-AGKGS) were also reported. The Pfk13 gene mutations associated with artemisinin resistance were not detected. This study highlights the role of ANC in achieving optimal SP coverage in pregnant women, the mitigated impact of IPTp-SP on malaria outcomes, and the high prevalence of multiple SP-resistant P. falciparum parasites in the city of Douala that could compromise the efficacy of IPTp-SP.<\/jats:p>","DOI":"10.3390\/pathogens12060844","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T03:04:40Z","timestamp":1687143880000},"page":"844","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effectiveness of Intermittent Preventive Treatment with Sulfadoxine-Pyrimethamine in Pregnancy: Low Coverage and High Prevalence of Plasmodium falciparum dhfr-dhps Quintuple Mutants as Major Challenges in Douala, an Urban Setting in Cameroon"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3252-6552","authenticated-orcid":false,"given":"Carole Else","family":"Eboumbou Moukoko","sequence":"first","affiliation":[{"name":"Malaria Research Unit, Centre Pasteur Cameroon, Yaound\u00e9 P.O. Box 1274, Cameroon"},{"name":"Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"},{"name":"Laboratory of Parasitology, Mycology and Virology, Postgraduate Training Unit for Health Sciences, Postgraduate School for Pure and Applied Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4286-147X","authenticated-orcid":false,"given":"Loick Pradel","family":"Kojom Foko","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, Dwarka, New Delhi 110077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e8le","family":"Ayina","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Centre Pasteur Cameroon, Yaound\u00e9 P.O. Box 1274, Cameroon"},{"name":"Pharmaceutical Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Tornyigah","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra P.O. Box 1181, Ghana"},{"name":"UMR 261 MERIT, Institut de Recherche pour le D\u00e9veloppement (IRD), Universit\u00e9 de Paris, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie Rachel","family":"Epote","sequence":"additional","affiliation":[{"name":"Haematology Laboratory, Centre Pasteur Cameroon, Yaound\u00e9 P.O. Box 1274, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1070-2108","authenticated-orcid":false,"given":"Ida Calixte","family":"Penda","sequence":"additional","affiliation":[{"name":"Clinical Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Epee Eboumbou","sequence":"additional","affiliation":[{"name":"Clinical Sciences Department, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"},{"name":"Pediatric Wards, Bonassama Hospital, Douala P.O. Box 9023, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge Bruno","family":"Ebong","sequence":"additional","affiliation":[{"name":"Animal Organisms Biology and Physiology Department, Faculty of Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9242-1018","authenticated-orcid":false,"given":"Gaetan","family":"Texier","sequence":"additional","affiliation":[{"name":"UMR 257-Vecteurs, Infections Tropicales et M\u00e9diterran\u00e9ennes-VITROME-IRD\/SSA\/AP-HM, Aix-Marseille University, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine Eveline","family":"Nsango","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Centre Pasteur Cameroon, Yaound\u00e9 P.O. Box 1274, Cameroon"},{"name":"Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence","family":"Ayong","sequence":"additional","affiliation":[{"name":"Malaria Research Unit, Centre Pasteur Cameroon, Yaound\u00e9 P.O. Box 1274, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicaise","family":"Tuikue Ndam","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra P.O. Box 1181, Ghana"},{"name":"UMR 261 MERIT, Institut de Recherche pour le D\u00e9veloppement (IRD), Universit\u00e9 de Paris, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Same Ekobo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala P.O. Box 24157, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ejogrb.2020.10.003","article-title":"Toxoplasmosis in pregnancy","volume":"255","author":"Ahmed","year":"2020","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/B978-0-444-64240-0.00005-2","article-title":"Neurologic infections during pregnancy","volume":"Volume 172","author":"Curcio","year":"2020","journal-title":"Handbook of Clinical Neurology"},{"key":"ref_3","unstructured":"WHO (World Health Organization) (2020). World Malaria Report, WHO. 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