{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:45:53Z","timestamp":1790837153560,"version":"4.1.0"},"reference-count":21,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["155904\/2019-1"],"award-info":[{"award-number":["155904\/2019-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The performance of a commercial immunofluorescence assay (IFA commercial), an in-house immunofluorescence assay (IFA in-house) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-C. burnetii in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (n = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The in-house IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). In-house IFA is an excellent alternative for diagnosing Q fever.<\/jats:p>","DOI":"10.3390\/pathogens12070873","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T05:39:13Z","timestamp":1687757953000},"page":"873","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1178-5643","authenticated-orcid":false,"given":"Danilo Alves de","family":"Fran\u00e7a","sequence":"first","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7886-1570","authenticated-orcid":false,"given":"Mateus de Souza Ribeiro","family":"Mioni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Fornazari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e1ssarah Jabur Lot","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Roberto Ferreira","family":"Polido","sequence":"additional","affiliation":[{"name":"Botucatu Medical School, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Michele","family":"Appolinario","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Let\u00edcia Devid\u00e9","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-9042","authenticated-orcid":false,"given":"Ana \u00cdris de Lima","family":"Dur\u00e9","sequence":"additional","affiliation":[{"name":"Ezequiel Dias Foundation, Ot\u00e1vio Magalh\u00e3es Institute, Belo Horizonte 30510-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Vinicius Ferreira","family":"Silva","sequence":"additional","affiliation":[{"name":"Ezequiel Dias Foundation, Ot\u00e1vio Magalh\u00e3es Institute, Belo Horizonte 30510-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8312-7397","authenticated-orcid":false,"given":"Virg\u00ednia Bodel\u00e3o","family":"Richini-Pereira","sequence":"additional","affiliation":[{"name":"Regional Laboratories Center II, Adolfo Lutz Institute, Bauru 17015-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":false,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Megid","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Public Health, S\u00e3o Paulo State University, Botucatu 05508-220, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"106087","DOI":"10.1016\/j.mimet.2020.106087","article-title":"Current approaches for the detection of Coxiella burnetii infection in humans and animals","volume":"179","author":"Sahu","year":"2020","journal-title":"J. Microbiol. Methods."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Beare, P.A., Jeffrey, B.M., Long, C.M., Martens, C.M., and Heinzen, R.A. (2018). Genetic mechanisms of Coxiella burnetii lipopolysaccharide phase variation. PLoS Pathog., 14.","DOI":"10.1371\/journal.ppat.1006922"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Fran\u00e7a, D.A., Mioni, M.S.R., Fornazari, F., Dur\u00e9, A.\u00cd.L., Silva, M.V.F., Possebon, F.S., Richini-Pereira, V.B., Langoni, H., and Megid, J. (2022). Seropositivity for Coxiella burnetii in suspected patients with dengue in S\u00e3o Paulo state, Brazil. PLoS Negl. Trop Dis., 16.","DOI":"10.1371\/journal.pntd.0010392"},{"key":"ref_4","first-page":"1","article-title":"Diagnosis and management of Q fever-United States, 2013: Recommendations from CDC and the Q Fever Working Group","volume":"62","author":"Anderson","year":"2013","journal-title":"MMWR Recomm. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S163","DOI":"10.1016\/j.ijtb.2020.07.034","article-title":"Benefits and limitations of serological assays in COVID-19 infection","volume":"67","author":"Sidiq","year":"2020","journal-title":"Indian J. Tuberc."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Toman, R., Heinzen, R.A., Samuel, J.E., and Mege, J.L. (2012). Coxiella burnetii: Recent Advances and New Perspectives in Research of the Q Fever Bacterium, Springer. [1st ed.].","DOI":"10.1007\/978-94-007-4315-1"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ullah, Q., Jamil, T., Saqib, M., Iqbal, M., and Neubauer, H. (2022). Q Fever\u2014A Neglected Zoonosis. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081530"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1590\/s1678-9946202365039","article-title":"Overview of Q fever in Brazil: An underestimated zoonosis","volume":"65","author":"Mioni","year":"2023","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.vetmic.2007.04.033","article-title":"Comparative diagnostic potential of three serological tests for abortive Q fever in goat herds","volume":"124","author":"Rousset","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1177\/1040638713517233","article-title":"Coxiella burnetii serology assays in goat abortion storm","volume":"26","author":"Emery","year":"2014","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1177\/1040638711416971","article-title":"Q fever diagnosis in domestic ruminants: Comparison between complement fixation and commercial enzyme-linked immunosorbent assays","volume":"23","author":"Horigan","year":"2011","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Caraguel, C., Bassett, S., Gonz\u00e1lez-Barrio, D., Elsworth, P., and Chaber, A.L. (2020). Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02526-w"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105859","DOI":"10.1016\/j.mimet.2020.105859","article-title":"Comparison of two new in-house Latex Agglutination Tests (LATs), based on the DnaK and Com1 synthetic peptides of Coxiella burnetii, with a commercial indirect-ELISA, for sero-screening of coxiellosis in bovines","volume":"170","author":"Yadav","year":"2020","journal-title":"J. Microbiol. Methods."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mioni, M.S.R., Sidi-Boumedine, K., Morales Dalanezi, F., Fernandes Joaquim, S., Denadai, R., Reis Teixeira, W.S., Bahia Labruna, M., and Megid, J. (2019). New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina. Pathogens, 9.","DOI":"10.3390\/pathogens9010030"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4269\/ajtmh.2004.71.93","article-title":"Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a Brazilian spotted fever-endemic area in the state of S\u00e3o Paulo, Brazil: Serologic evidence for infection by Rickettsia rickettsii and another spotted fever group Rickettsia","volume":"71","author":"Horta","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The measurement of observer agreement for categorical data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"ref_17","unstructured":"Dean, A.G., Sullivan, K.M., and Soe, M.M. (2022, May 20). Open Source Epidemiologic Statistics for Public Health. Available online: https:\/\/www.openepi.com\/SampleSize\/SSPropor.htm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1017\/S0950268897008510","article-title":"Molecular characterization of Coxiella burnetii isolates","volume":"120","author":"Willems","year":"1998","journal-title":"Epidemiol. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1128\/JB.188.7.2309-2324.2006","article-title":"Genetic diversity of the Q fever agent, Coxiella burnetii, assessed by microarray-based whole-genome comparisons","volume":"188","author":"Beare","year":"2006","journal-title":"J. Bacteriol."},{"key":"ref_20","first-page":"459","article-title":"Study of cross-reaction between Coxiella burnetii and Legionella pneumophila using indirect immunofluorescence assay and immunoblotting","volume":"36","author":"Finidori","year":"1992","journal-title":"Acta Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/jcm.34.9.2270-2274.1996","article-title":"Serological cross-reactions between Bartonella quintana, Bartonella henselae, and Coxiella burnetii","volume":"34","author":"Raoult","year":"1996","journal-title":"J. Clin. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/7\/873\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:00:43Z","timestamp":1760126443000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/7\/873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,26]]},"references-count":21,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["pathogens12070873"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12070873","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,26]]}}}