{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:49:02Z","timestamp":1791384542856,"version":"4.3.3"},"reference-count":387,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Successfully developed in 1976, the continuous in vitro culture of Plasmodium falciparum has many applications in the field of malaria research. It has become an important experimental model that directly uses a human pathogen responsible for a high prevalence of morbidity and mortality in many parts of the world and is a major source of biological material for immunological, biochemical, molecular, and pharmacological studies. Until present, the basic techniques described by Trager and Jensen and Haynes et al. remain unchanged in many malaria research laboratories. Nonetheless, different factors, including culture media, buffers, serum substitutes and supplements, sources of erythrocytes, and conditions of incubation (especially oxygen concentration), have been modified by different investigators to adapt the original technique in their laboratories or enhance the in vitro growth of the parasites. The possible effects and benefits of these modifications for the continuous cultivation of asexual intraerythrocytic stages of P. falciparum, as well as future challenges in developing a serum-free cultivation system and axenic cultures, are discussed.<\/jats:p>","DOI":"10.3390\/pathogens12070900","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T01:03:02Z","timestamp":1688346182000},"page":"900","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8812-8185","authenticated-orcid":false,"given":"Leonardo K.","family":"Basco","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement (IRD), Assistance Publique\u2014H\u00f4pitaux de Marseille (AP-HM), Service de Sant\u00e9 des Arm\u00e9es (SSA), Unit\u00e9 Mixte de Recherche (UMR) Vecteurs\u2014Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), 13005 Marseille, France"},{"name":"Institut Hospitalo-Universitaire\u2014M\u00e9diterran\u00e9e Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,1]]},"reference":[{"key":"ref_1","first-page":"95","article-title":"Malaria in its various vertebrate hosts","volume":"Volume 1","author":"Kreier","year":"1980","journal-title":"Malaria: Epidemiology, Chemotherapy, Morphology, and Metabolism"},{"key":"ref_2","first-page":"1","article-title":"The importance of malaria in the world","volume":"Volume 1","author":"Kreier","year":"1980","journal-title":"Malaria: Epidemiology, Chemotherapy, Morphology, and Metabolism"},{"key":"ref_3","unstructured":"Warrell, D.A., and Gilles, H.M. 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