{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T11:21:53Z","timestamp":1783164113912,"version":"3.54.6"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The present retrospective study analyzed the characteristics of strongyloidiasis in patients who were diagnosed at the Outpatient Clinic of the Department of Parasitology-Mycology, Toulouse, France. Sixty-nine file records were included in the study on the basis of a positive stool examination that used Baermann\u2019s method. The prominent epidemiological findings were the presence of former immigrants from Italy or Portugal, veterans from the 1st Indochina war, and autochthonous cases. Almost 1\/4 of the patients were asymptomatic. Manifestations of skin allergy were the main clinical feature. Blood eosinophilia was present in 76.8% of the patients, and serum total IgE was \u2265150 kIU\/L in 79.7%. Immunodiagnosis was achieved from 1990 to 2001 by indirect immunofluorescence (IFAT) that was then replaced with ELISA, both methods using Strongyloides ratti filariform larvae. ELISA was found to be similar to IFAT in terms of specificity but exhibited a greater sensitivity. Patients were primarily treated with albendazole or ivermectin beginning in 1993. Forty-eight patients attended the follow-up consultation. Kinetics of the clinical picture and blood eosinophilia were found to be the most convenient parameters to assess the efficacy of anthelmintic therapy. In conclusion, strongyloidiasis remains a neglected disease in Southwestern France. The resolution of clinical features along with the kinetics of eosinophilia appeared to be the most appropriate parameters to check during the posttreatment follow-up.<\/jats:p>","DOI":"10.3390\/pathogens12080983","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T02:10:45Z","timestamp":1690510245000},"page":"983","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Epidemiological, Clinical and Laboratory Features of Strongyloidiasis in 69 Attendees at a French Outpatient Clinic"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7759-894X","authenticated-orcid":false,"given":"Jean-Fran\u00e7ois","family":"Magnaval","sequence":"first","affiliation":[{"name":"Service de Parasitologie M\u00e9dicale, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Toulouse, 31000 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5277-189X","authenticated-orcid":false,"given":"Judith","family":"Fillaux","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Purpan, Centre Hospitalier Universitaire de Toulouse, TSA 4003, 31059 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Fabre","sequence":"additional","affiliation":[{"name":"DENDRIS, 335 Rue du Ch\u00eane Vert, 31670 Lab\u00e8ge, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0055-0136","authenticated-orcid":false,"given":"Sophie","family":"Cassaing","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Purpan, Centre Hospitalier Universitaire de Toulouse, TSA 4003, 31059 Toulouse, France"},{"name":"FLAMES\/RESTORE (Inserm UMR 1301\/CNRS UMR 5070\/EFS), Universit\u00e9 de Toulouse, 31100 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Valentin","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Purpan, Centre Hospitalier Universitaire de Toulouse, TSA 4003, 31059 Toulouse, France"},{"name":"PHARMA-DEV (UMR 152), Institut de Recherche Pour le D\u00e9veloppement, Universit\u00e9 de Toulouse, 31062 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Iriart","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Purpan, Centre Hospitalier Universitaire de Toulouse, TSA 4003, 31059 Toulouse, France"},{"name":"Institut Toulousain des Maladies Infectieuses et Inflammatoires (UMR \u201cInfinity\u201d, Inserm\/CNRS\/Universit\u00e9 de Toulouse III), 31024 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Berry","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, H\u00f4pital Purpan, Centre Hospitalier Universitaire de Toulouse, TSA 4003, 31059 Toulouse, France"},{"name":"Institut Toulousain des Maladies Infectieuses et Inflammatoires (UMR \u201cInfinity\u201d, Inserm\/CNRS\/Universit\u00e9 de Toulouse III), 31024 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0065-308X(08)60036-6","article-title":"Human strongyloidiasis","volume":"38","author":"Grove","year":"1996","journal-title":"Adv. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Jaleta, T.G., Zhou, S., Bemm, F.M., Sch\u00e4r, F., Khieu, V., Muth, S., Odermatt, P., Lok, J.B., and Streit, A. (2017). Different but overlapping populations of Strongyloides stercoralis in dogs and humans\u2014Dogs as a possible source for zoonotic strongyloidiasis. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005752"},{"key":"ref_3","unstructured":"Warren, K.S., and Mahmoud, A.A.F. (2000). Tropical and Geographical Medicine, McGraw Hill. [2nd ed.]."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1001\/archderm.1958.01560080044007","article-title":"Larva currens: A distinctive variant of cutaneous Larva migrans due to Strongyloides stercoralis","volume":"78","author":"Arthur","year":"1958","journal-title":"AMA Arch. Derm."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1097\/QCO.0b013e3283551dbd","article-title":"Screening, prevention, and treatment for hyperinfection syndrome and disseminated infections caused by Strongyloides stercoralis","volume":"25","author":"Mejia","year":"2012","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s12639-019-01090-x","article-title":"Strongyloides stercoralis hyperinfection syndrome: A deeper understanding of a neglected disease","volume":"43","author":"Marin","year":"2019","journal-title":"J. Parasit. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/CMR.5.4.345","article-title":"Dysregulation of strongyloidiasis: A new hypothesis","volume":"5","author":"Genta","year":"1992","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.3201\/eid2807.220198","article-title":"Strongyloides hyperinfection syndrome among COVID-19 Patients treated with corticosteroids","volume":"28","author":"Kim","year":"2022","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1590\/S0036-46651993000400010","article-title":"Total serum IgE and parasite-specific IgG and IgA antibodies in human strongyloidiasis","volume":"35","author":"Rossi","year":"1993","journal-title":"Rev. Inst. Med. Trop. Sao Paulo."},{"key":"ref_10","unstructured":"Grove, D.I. (1989). Strongyloidiasis: A Major Roundworm Infection of Man, Taylor & Francis."},{"key":"ref_11","first-page":"15","article-title":"A diagnostic protocol designed for determining allergic causes in patients with blood eosinophilia","volume":"4","author":"Magnaval","year":"2017","journal-title":"Mil. Med. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/BF02231086","article-title":"Diagnosis of strongyloidiasis: Importance of Baermann\u2019s method","volume":"6","author":"Delgado","year":"1961","journal-title":"Dig. Dis. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1086\/322707","article-title":"Diagnosis of Strongyloides stercoralis infection","volume":"33","author":"Siddiqui","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1128\/jcm.34.10.2569-2571.1996","article-title":"Patterns of detection of Strongyloides stercoralis in stool specimens: Implications for diagnosis and clinical trials","volume":"34","author":"Dreyer","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1017\/S0031182016002559","article-title":"Parasitology. A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection","volume":"144","author":"Buonfrate","year":"2017","journal-title":"Parasitology"},{"key":"ref_16","first-page":"74","article-title":"Diagnostic s\u00e9rologique de l\u2019anguillulose humaine par immuno-fluorescence (R\u00e9sultats pr\u00e9liminaires)","volume":"61","author":"Coudert","year":"1968","journal-title":"Bull. Soc. Pathol. Exot."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"344","DOI":"10.4269\/ajtmh.1981.30.344","article-title":"Diagnosis of human strongyloidiasis by immunofluorescence, using Strongyloides ratti and S. stercoralis larvae","volume":"30","author":"Grove","year":"1981","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/CVI.00278-06","article-title":"Evaluation of an indirect immunofluorescence assay for strongyloidiasis as a tool for diagnosis and follow-up","volume":"14","author":"Boscolo","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1023\/A:1007632028471","article-title":"A retrospective study of autochthonous strongylo\u00efdiasis in R\u00e9gion Midi-Pyr\u00e9n\u00e9es (Southwestern France)","volume":"16","author":"Magnaval","year":"2000","journal-title":"Eur. J. Epidemiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Sears, W.J., and Nutman, T.B. (2022). Strongy Detect: Preliminary validation of a prototype recombinant Ss-NIE\/Ss-IR based ELISA to detect Strongyloides stercoralis infection. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010126"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.cmi.2015.04.001","article-title":"Novel approaches to the diagnosis of Strongyloides stercoralis infection","volume":"21","author":"Buonfrate","year":"2015","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s13071-023-05763-8","article-title":"From past to present: Opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis","volume":"16","author":"Chan","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1007\/s00436-018-5985-5","article-title":"Strongyloides stercoralis: Detection of parasite-derived DNA in serum samples obtained from immunosuppressed patients","volume":"117","author":"Kalantari","year":"2018","journal-title":"Parasitol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.4269\/ajtmh.18-0895","article-title":"Morbidity associated with chronic Strongyloides stercoralis infection: A systematic review and meta-analysis","volume":"100","author":"Tamarozzi","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_25","unstructured":"Ash, L.R., and Orihel, T.C. (1991). Parasites, a Guide to Laboratory Procedures and Identification, ASCP Press. [1st ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.4269\/ajtmh.1955.4.23","article-title":"A new concentration technic for the demonstration of protozoa and helminth eggs in feces","volume":"4","author":"Blagg","year":"1955","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.4269\/ajtmh.1987.37.157","article-title":"Comparative sensitivity and specificity of ELISA and IHA for serodiagnosis of strongyloidiasis with larval antigens","volume":"37","author":"Gam","year":"1987","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_28","first-page":"87","article-title":"Antibodies to Strongyloides stercoralis larval surface antigens in chronic strongyloidiasis","volume":"47","author":"Genta","year":"1982","journal-title":"Lab. Investig."},{"key":"ref_29","first-page":"459","article-title":"Application of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis","volume":"31","author":"Speiser","year":"1980","journal-title":"Tropenmed. Parasitol."},{"key":"ref_30","first-page":"619","article-title":"Treatment of Strongyloides stercoralis with albendazole. A cure rate of 86 per cent","volume":"263","author":"Mojon","year":"1987","journal-title":"Zentralbl. Bakteriol. Mikrobiol. Hyg. A"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"310","DOI":"10.2169\/internalmedicine.31.310","article-title":"Efficacy of ivermectin against Strongyloides stercoralis in humans","volume":"31","author":"Shikiya","year":"1992","journal-title":"Intern. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1093\/infdis\/169.5.1076","article-title":"A randomized trial of single- and two-dose ivermectin versus thiabendazole for treatment of strongyloidiasis","volume":"169","author":"Gann","year":"1994","journal-title":"J. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.trstmh.2010.06.001","article-title":"Clinical and epidemiological features of 33 imported Strongyloides stercoralis infections","volume":"104","author":"Gallo","year":"2010","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"428","DOI":"10.4269\/ajtmh.19-0087","article-title":"Clinical and diagnostic features of 413 patients treated for imported strongyloidiasis at the Hospital for Tropical Diseases, London","volume":"101","author":"Ming","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"80","DOI":"10.2310\/7060.2005.12204","article-title":"Imported strongyloidosis: A longitudinal analysis of 31 cases","volume":"12","author":"Nuesch","year":"2005","journal-title":"J. Travel Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ijid.2013.09.009","article-title":"Imported strongyloidiasis in Spain","volume":"18","author":"Espinosa","year":"2014","journal-title":"Int. J. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Puthiyakunnon, S., Boddu, S., Li, Y., Zhou, X., Wang, C., Li, J., and Chen, X. (2014). Strongyloidiasis\u2014An insight into its global prevalence and management. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0003018"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.ijid.2020.09.002","article-title":"Modeling strongyloidiasis risk in the United States","volume":"100","author":"Singer","year":"2020","journal-title":"Int. J. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ottino, L., Buonfrate, D., Paradies, P., Bisoffi, Z., Antonelli, A., Rossolini, G.M., Gabrielli, S., Bartoloni, A., and Zam-marchi, L. (2020). Autochthonous human and canine Strongyloides stercoralis infection in Europe: Report of a human case in an Italian teen and systematic review of the literature. Pathogens, 9.","DOI":"10.3390\/pathogens9060439"},{"key":"ref_40","first-page":"328","article-title":"Autochthonous strongyloidiasis in Italy: An epidemiological and clinical review of 150 cases","volume":"77","author":"Scaglia","year":"1984","journal-title":"Bull. Soc. Pathol. Exot."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30310","DOI":"10.2807\/1560-7917.ES.2016.21.31.30310","article-title":"CCM Strongyloides Study Group. Epidemiology of Strongyloides stercoralis in northern Italy: Results of a multicentre case-control study, February 2013 to July 2014","volume":"21","author":"Buonfrate","year":"2016","journal-title":"Euro Surveill."},{"key":"ref_42","first-page":"85","article-title":"Ocorr\u00eancia da estrongiloidose aut\u00f3ctone em Portugal. S\u00edntese retrospectiva","volume":"8","year":"2012","journal-title":"RPDI"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.1017\/S0950268817002333","article-title":"Strongyloides stercoralis seroprevalence in Vietnam","volume":"145","author":"Diep","year":"2017","journal-title":"Epidemiol. Infect."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1001\/jama.1987.03400010053026","article-title":"Strongyloidiasis in US veterans of the Vietnam and other wars","volume":"258","author":"Genta","year":"1987","journal-title":"JAMA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/1753-6405.12360","article-title":"Seroprevalence of Strongyloides stercoralis in a South Australian Vietnam veteran cohort","volume":"39","author":"Rahmanian","year":"2015","journal-title":"Aust. N. Z. J. Public Health"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1016\/j.cmi.2021.07.016","article-title":"Clinical and laboratory features of Strongyloides stercoralis infection at diagnosis and after treatment: A systematic review and meta-analysis","volume":"27","author":"Buonfrate","year":"2021","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1016\/S0140-6736(68)90187-6","article-title":"Immunoglobulin levels in Ethiopian preschool children with special reference to high concentrations of immunoglobulin E (IgND)","volume":"1","author":"Johansson","year":"1968","journal-title":"Lancet"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1136\/bmj.280.6214.598","article-title":"Strongyloidiasis in Allied ex-prisoners of war in south-east Asia","volume":"280","author":"Grove","year":"1980","journal-title":"Br. Med. J."},{"key":"ref_49","first-page":"CD007745","article-title":"Ivermectin versus albendazole or thiabendazole for Strongyloides stercoralis infection","volume":"2016","author":"Gotuzzo","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.jaci.2016.04.056","article-title":"Half-life of IgE in serum and skin: Consequences for anti-IgE therapy in patients with allergic disease","volume":"139","author":"Lawrence","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1051\/parasite\/2001084359","article-title":"A three years follow-up of total serum IgE levels in three patients treated for strongyloidiasis","volume":"8","author":"Poirriez","year":"2001","journal-title":"Parasite"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/983\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:20:39Z","timestamp":1760127639000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/983"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,27]]},"references-count":51,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["pathogens12080983"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12080983","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,27]]}}}